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Jessa Hughes, Hamid Khazaei, and Albert Vandenberg

. 89–114. In: J. Janick (ed.). Plant Breeding Reviews. vol. 25. John Wiley & Sons, Inc., Hoboken, NJ Gutierrez, N. Torres, A.M. 2019 Characterization and diagnostic marker for TTG1 regulating tannin and anthocyanin biosynthesis in faba bean Sci. Rep

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Juan M. Osorno, Carlos G. Muñoz, James S. Beaver, Feiko H. Ferwerda, Mark J. Bassett, Phil N. Miklas, Teresa Olczyk, and Bill Bussey

Methods The advance-generation common bean breeding line I9557-9 was derived from the backcross HP8437-95 × G35172 × HP8437-95 made at the U.S. Department of Agriculture, Agricultural Research Service Tropical Agriculture Research Station in 1993. HP8437

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D.H. Wallace, Paul A. Gniffke, P.N. Masaya, and R.W. Zobel

. of Plant Breeding Paper no. 783. This research was supported by Hatch funds and the Bean/Cowpea Collaborative Research Program funded by USAID. The research also used fields, facilities, and resources of the Centro International de Agricultural

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Mark J. Bassett

A cross was made between gri (gray-white seedcoat) and p (pure-white seedcoat) using genetic stocks gri BC2 5-593 and p BC2 5-593 developed to carry only a single recessive allele for seedcoat color in an otherwise all-dominant genetic background. The recurrent parent, 5-593, is a Florida dry-bean breeding line with bishops-violet flowers, determinate habit, small seed size, shiny black seeds, and seedcoat genotype T Mar P [C r] D J G B V Rk. The F1 progeny from the above cross between gri and p had the flower color pattern and seedcoat color of the griseoalbus character (gri), but had less intense color expression. Therefore, I hypothesized that gri is an allele at the P locus (allelic interaction). The hypothesis of allelism was confirmed in the F2, which failed to segregate for bishops-violet flowers and black seed, i.e., no complementation was evident. The symbol p gri is proposed for the new allele at P, where the dominance series is P > p gri > p. The gene for gray-white seeds in gri BC2 5-593 was shown to be allelic to Lamprecht's gri gene in V0059 (PI 527716).

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Zhanyuan Zhang, A. Mitra, and D.P. Coyne

Factors influencing Agrobacterium–mediated DNA transfer of P. vulgaris were examined using an intron-containing β-glucuronidase (GUS) gene as a reporter system. Tissue culture procedures used were based on direct shoot organogenesis. Two A. tumefaciens strains, A2760 and EHA105, were used with more emphasis on the former due to its overall higher transformation rate. Ten bean entries including breeding lines and cultivars from both Meso-American and Andean origins were compared for compatibility with the two bacterial strains under different pre- and coculture conditions. Pinto `Othello' was extensively used in testing different transformation conditions. Factors found to have significant effects on transformation rate included Agrobacterium-host interactions, explant maturity, preculture and cocultivation conditions, as well as selection schemes, based on transient expression. Some factors, such as the effect of explant maturity and dark preconditioning of explants on gene transfer, have not been reported before. The best transformation conditions included the use of susceptible genotypes and mature explants, preconditioning of explants in darkness, followed by a maximum cocultivation period in the presence of cytokinin, and the use of high selection pressure.

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Mark J. Bassett

Common bean (Phaseolus vulgaris L.) plant introduction 527829 (formerly Lamprecht M0048) has dark seal-brown (DSB) seedcoats and pink flowers. An investigation was conducted to determine the genotype of DSB seedcoat color. M0048 was crossed with Florida breeding line 5-593, which has genotype P [C r] D J G B V Rk. A series of crosses involving M0048, 5-593, and three genetic tester stocks (v BC2 5-593, c u BC2 5-593, and b v BC2 5-593) led to determination of the genotype. Data analysis indicated that M0048 has the genotype P [? R] J G B v lae, where DSB color is produced by the interaction of R with B. Crosses between [? R] and testers with [C r] always produced seedcoat mottling in F1, except where V masks the effect. The cross [? R] B v (DSB) × c u BC2 5-593 (cartridge buff seedcoat) produced marbled seedcoats (black/cartridge buff) with genotype [? R]/[c u ?] B V. No way was found to determine whether the mottled or marbled seedcoat patterns were controlled at C or R; hence, the allelic ambiguity is indicated with a question mark. Illustrations are provided showing the difference between seedcoat mottling (a highly variable low-contrast patterning) and seedcoat marbling (a less variable high-contrast patterning, usually with cartridge buff as the background color). The development of a new genetic tester stock, [? R] b v BC3 5-593, was described, where [? R] b v gives unpatterned dominant red seedcoat color.

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Eladio Arnaud-Santana, D.P. Coyne, K.M. Eskridge, and A.K. Vidaver

and head, Dept. of Plant Pathology. Published as Journal series paper no. 10230, Nebraska Agricultural Research Division. Research was conducted under Title XII Bean-Cowpea CRSP Project, Univ. of Nebraska, Univ. of Puerto Rico, and Dominican Republic

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Timothy G. Porch, Matthew W. Blair, Patricia Lariguet, Carlos Galeano, Clive E. Pankhurst, and William J. Broughton

thus important. Radioisotopes, x-ray, and chemical mutagens have been used previously to induce mutations in common bean. Gamma radiation has been used for the breeding of seed yield and quality ( Sarafi, 1973 ), seed color ( Allavena, 1989 ; Hussein

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Harry C. Bittenbender, Loren D. Gautz, Ed Seguine, and Jason L. Myers

components for growth. Heat and acids produced by microorganisms kill the embryo before the radical emerges. When the fermented beans are roasted, the flavor compounds associated with chocolate are produced ( Schwan and Wheals, 2004 ). Breeding and variety

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Elisha Otieno Gogo, Mwanarusi Saidi, Jacob Mugwa Ochieng, Thibaud Martin, Vance Baird, and Mathieu Ngouajio

. Ndegwa, A. 2004 Breeding snap beans for smallholder production in east and central Africa, p. 49–51. In: Bean improvement for the tropics, annual report 2004. CIAT, Cali, Colombia Licciardi, S. Assogba-Komlan, F. Sidick, I. Chandre, F. Hougard, J