Search Results

You are looking at 101 - 110 of 150 items for :

  • All content x
Clear All
Full access

Yuji Yamada, Masayoshi Nakayama, Hiromitsu Shibata, Sanae Kishimoto, and Takashi Ikeda

the retention times and absorption spectra (400–510 nm) were compared. RNA extraction and quantitative polymerase chain reaction (qPCR). Total RNA extraction was performed following the methods of Matsushita et al. (2016) and Okutsu et al. (2018

Free access

Alexis K. Nagel, Guido Schnabel, Cesar Petri, and Ralph Scorza

been engineered for tolerance to viruses, insects, or herbicides ( Schahczenski and Adam, 2006 ). Currently, the main transgenic crops to be cultivated globally are soybean, maize, and cotton ( James, 2003 ). Woody species are now being targeted for

Free access

Jiao Chen, De-bao Yuan, Chao-zheng Wang, Yi-xing Li, Fen-fang Li, Ke-qian Hong, and Wang-jin Lu

jasmonic acid-responsive genes ( Serrano and Guzman, 2004 ). Transgenic tobacco ( Nicotiana tabacum ) plants that constitutively express rice ( Oryza sativa ) RING gene OsBIRF1 exhibited enhanced disease resistance against Tobacco mosaic virus and

Open access

Tripti Vashisth and Taylor Livingston

diseases. Such treatment of heat is often referred to as thermotherapy and has been reported to be effective against pathogenic microorganisms like viruses, bacteria, fungi, etc. ( Grondeau et al., 1994 ). The principle of thermotherapy is that “parasitic

Free access

Julio Solis, Arthur Villordon, Niranjan Baisakh, Don LaBonte, and Nurit Firon

Methods Plant material. Sweetpotato plant cuttings (≈20 cm long) were obtained from generation 0 (derived from in vitro cultures) virus-tested, greenhouse-grown ‘Beauregard’ for greenhouse studies and virus-tested generation 1 seed roots in plant beds for

Free access

Steven J. Damon and Michael J. Havey

Onion thrips is an important insect pest of onion causing damage to foliage and reducing bulb and seed yields ( Diaz-Montano et al., 2010 ; Elmore, 1949 ). Thrips also vector serious diseases such as iris yellow spot virus ( Gent et al., 2004

Free access

Moon Kyung Ko, Hyunchul Soh, Kyung-Moon Kim, Young Soon Kim, and Kyunghoan Im

isolated total RNA was hybridized with hpt probe DNA. At the mRNA level, the introduced transgene was transcriptionally expressed in the two primary transgenic lines as well as their T 1 progenies, whereas no signal was detected in the wild type ( Fig. 4

Free access

Shanshan Seng, Jian Wu, Jiahui Liang, Fengqin Zhang, Qiuyan Yang, Junna He, and Mingfang Yi

. Nicotiana benthamiana seeds were planted into pots and cultured under the same conditions as the arabidopsis. Isolation and sequence analysis of GhAGPL1. RNA extraction kit (Tiangen, Beijing, China) was used to extract total RNA from gladiolus cormels

Full access

Guangxin Liu, Xiaoqian Su, Lingling Guan, and Fengrong Hu

the leaves of resistant and susceptible cotton ( Gossypium hirsutum ) genotypes infected by Cotton leaf curl Burewala virus . Their results showed that PAL, POD, CAT, and SOD have active roles in disease resistance against Cotton leaf curl Burewala

Free access

Kelly J. Vining, Q Zhang, C.A. Smith, and T.M. Davis

has motifs of amino acid sequences LQKKLLSKLL (called RNBS-A-TIR) and FLHIACFF (called RNBS-D-TIR), whereas the non-TIR class has FDLXAWVCVSQXF (called RNAS-A-non-TIR) and CFLYCALFPED (called RNAS-D-non-TIR) ( Meyers et al., 1999 ). In addition, the