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Dan Drost, Taunya Ernst, and Brent Black

. After sunset, and when conditions were cold and clear, both secondary covers retained more heat at night. Although night air temperatures during fall and winter production periods within the HT often dropped below freezing, secondary covers were able to

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Sharon Sowa, Eric E. Roos, and Francis Zee

Seeds of the recalcitrant species Litch i chinénis and Euphoria longan were stored in humid conditions at 8-10C under three different atmospheres: air, 80% nitrous oxide (N20)/20 % oxygen, and 100% nitrous oxide. The combination of anesthetic and oxygen extended storage longevity of both species. Oxygen was required for maintenance of viability; seeds stored under 100% N20 lost germinability at the most rapid rate. Lychee seeds retained 92% of control germination after 12 weeks under 80% N20/20% 02, while those under air lost 56% viability. Longan seeds lost all viability after 7 weeks under air, yet retained 70% of their control germination under 80% N20/20% 02. The combination of anesthetic and oxygen atmospheres could provide a new approach to recalcitrant seed storage.

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Marcos D. Ferreira, Jeffrey K. Brecht, Steven A. Sargent, and Craig K. Chandler

Hydrocooling was evaluated as an alternative to forced-air cooling for strawberry (Fragaria × ananassa) fruit. `Sweet Charlie' strawberries were cooled by forced-air and hydrocooling to 4 °C and held in different storage regimes in three different trials. Quality attributes, including surface color, firmness, weight loss, soluble solids, and ascorbic acid content, pH and total titratable acidity, were evaluated at the full ripe stage. Fruit hydrocooled to 4 °C and stored at different temperatures for 8 or 15 days showed overall better quality than forced-air cooled fruit, with significant differences in epidermal color, weight loss, and incidence and severity of decay. Fruit stored wrapped in polyvinylchloride (PVC) film after forced-air cooling or hydrocooling retained better color, lost less weight, and retained greater firmness than fruit stored uncovered, but usually had increased decay. There is potential for using hydrocooling as a cooling method for strawberries.

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George L. Hosfield and Clifford W. Beninger

Seed coat color in dry bean (Phaseolus vulgaris L.) is determined by the presence or absence of tannins, flavonoids, and anthocyanins. Black beans contain three main anthocyanins that are responsible for their black seed coat color: delphinidin 3-O-glucoside, petunidin 3-O-glucoside, and malvidin 3-O-glucoside. Leaching of anthocyanins occurs in many black bean genotypes during thermal processing (i.e., blanching and cooking). Black beans that lose their dark color after processing are unacceptable to the industry. Since the marketability of black beans can be adversely affected by thermal processing, an experiment was conducted to ascertain whether pigment leaching was due to qualitative or quantitative changes in anthocyanins during processing. Four black bean genotypes that showed differential leaching of color were investigated. `Harblack' retains most of its black color after processing while `Raven' loses most of its color. `Black Magic' and `Black Jack' are intermediate between `Harblack' and `Raven' in processed color. Bean samples (119 ± 1.5 g) of the four genotypes were thermally processed in 100 x 75-mm tin cans in a pilot laboratory. Seed coats were removed from the cooked beans, freeze-dried, and placed in solutions of formic 10 acid: 65 water: 25 methanol to extract anthocyanins. The extracts were analyzed by HPLC. Although all genotypes retained some color, there were no detectable anthocyanins in seed coats of the cooked beans. In a second experiment, raw beans of each genotype were boiled in distilled water for 15 minutes. All four genotypes lost color during boiling, but `Harblack' retained most of its color and had a five-fold higher concentration of the three anthocyanins than did the other genotypes. `Harblack' may retain color better than other black beans because of physical characteristics of the seed coat.

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Hannah M. Mathers, Luke T. Case, and Thomas H. Yeager

As limitations on water used by container nurseries become commonplace, nurseries will have to improve irrigation management. Several ways to conserve water and improve on the management of irrigation water applied to container plants are discussed in this review. They include 1) uniform application, 2) proper scheduling of irrigation water, 3) substrate amendments that retain water, 4) reducing heat load or evaporative loss from containers, and 5) recycling runoff water.

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R. Keith Striegler, Justin R. Morris, Gary L. Main, Chris B. Lake, Simon R. Graves, Renee T. Threlfall, and Janice M. Blevins

`Sunbelt' is a juice grape cultivar developed by the Univ. of Arkansas. This cultivar produces `Concord'-type juice and is adapted to climatic conditions of the southern United States. Preliminary evaluation showed that `Sunbelt' has potential to produce high-quality juice under the hot climatic conditions of the San Joaquin Valley. A study was conducted during the 1998 and 1999 seasons to further evaluate the adaptation of `Sunbelt' to San Joaquin Valley conditions and determine the response of this cultivar to selected pruning methods. Vines of uniform vine size and vigor were subjected to four pruning treatments: severe hand-pruning (60 to 80 nodes retained/vine); moderate hand-pruning (120 to 160 nodes retained/vine); machine-pruning (160 to 180 nodes retained/vine); and minimal pruning (200 to 400 nodes retained/vine). Vines were trained to a Geneva Double Curtain trellis system. Yield and components of yield were significantly impacted by pruning treatment. In both seasons, mechanized systems of pruning (machine or minimal) produced higher yield than hand pruning. Minimal pruning resulted in the highest yield in 1998, while yield from machine-pruned vines was highest in 1999. Minimally pruned vines had the highest clusters/vine, lowest cluster weight, and lowest berry weight among the treatments. Fruit composition was also affected by pruning treatment. Minimal pruning produced fruit which was less mature than fruit from the other treatments in 1998. This result was likely due to the high yield obtained. Few differences in fruit composition were observed among treatments in 1999. The effect of pruning method on processed juice quality will be presented. Acceptable juice quality was obtained for most treatments.

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G.A. Picchioni, S.A. Weinbaum, and P.H. Brown

Leaf retention, uptake kinetics, total uptake (per unit leaf area), export kinetics, and the total export of foliage-applied, labeled B (]0B-enriched boric acid) were determined for apple (Malus domestics Borkh.), pear (Pyrus communis L.), prune (Prunus domestics L.), and sweet cherry (P. avium L.). Foliar uptake of labeled B by shoot leaves was 88% to 96% complete within 24 hours of application. More than 50% of the B retained on shoot leaf surfaces following application was absorbed and exported within 6 hours of application. Genotypic differences in shoot leaf surface characteristics among the species tested greatly influenced the amount of solution retained per unit leaf area. Leaf retention capacity was the primary determinant of the quantity of B absorbed by and exported from shoot leaves following foliar application. On average, apple shoot leaves retained, absorbed, and exported at least twice as much labeled B per unit leaf area as prune and pear shoot leaves and three to four times as much as sweet cherry shoot leaves. The sink demand of nearby, mature apples did not affect the export of labeled B when applied to adjacent spur leaves, but the fruit imported 16% of their total B from the applied solution during a 10-day period. Despite extensive documentation for the immobility of B accumulated by leaves naturally (e.g., from the soil), the B accumulated by leaves following foliage application was highly mobile in all four species tested.

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Robert R. Krueger and Mikeal L. Roose

New potential citrus germplasm accessions may be received as seed rather than budwood, thereby reducing phytosanitary risks. However, trueness-to-type may be an issue with seed materials because many varieties produce both apomictic (nucellar) and sexual (zygotic) embryos and most citrus is fairly heterozygous. To identify nucellar seedlings of polyembryonic types and to retain these as representing the type, we screened 1340 seedlings from 88 seed sources for markers amplified with two inter-simple sequence repeat (ISSR) primers. Sixteen seed sources produced no seedlings classified as being of nucellar origin. Among the remaining seed sources, seedlings classed as nucellar were identified for potential addition to the collection. In 37 accessions, both nucellar and zygotic seedlings were detected, and in some cases both types were retained. Inclusion of established accessions of the same cultivar group in the analysis allowed an initial assessment of similarity to existing accessions. This technique improved the efficiency of acquiring new germplasm of polyembryonic types by seed. The method identifies those seed sources that produce few or no nucellar seedlings, but it is not useful for determining which seedlings of monoembryonic types should be retained in collections.

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Martin C. Goffinet, Alan N. Lakso, and Terence L. Robinson

For 4 years, six-flowered clusters on 20, unthinned, open-pollinated `Empire'/MM106 trees were labeled at bloom and fruit drop monitored at the king (K) and lateral positions L1 (basal) to L5 (distal) (100 to 120 clusters/year). Depending on year, fruit dropped in 1, 2, or 3 major periods by 8 weeks postbloom (PB), with total percent dropped between 65% and 75%. K fruit dropped least, L4 and L5 most. Trends were that K fruit at October harvest were largest and heaviest (significantly so in some years) and L5 fruit smallest. In nine trees, hand-thinned to single-fruited spurs at 12 days PB, where the fruit at the retained position was known, there was no statistical difference in fruit weight, fruit size, or seed count between cluster positions at final harvest, although L5 fruit tended to be smallest. Numbers of spurs labeled varied from 45 to 72. Percent fruit retained at each position at October harvest was K = 89%, L1 or L2 = 87%, L3 = 83%, L4 = 83%, and L5 = 85%. Presumably, in unthinned trees the limited resources are preferentially taken by the K fruit, which especially seems to reduce set and size of its nearest lateral fruit. However, in thinned trees under lighter cropping stresses, a fruit retained at any of the positions within a cluster has a similar potential for achieving the size and weight typically seen in king fruit.

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Marion J. Packett, Alex X. Niemiera, J. Roger Harris, and Ronald F. Walden

Growers report that plants on gravel bed surfaces require more frequent irrigation compared to plastic surfaces. The objective of Expt. 1 was to determine if bed surface type influenced container environment and plant growth of azalea and Japanese holly plants on plastic- or gravel-covered beds. Measurements included bed, substrate, and plant canopy temperatures; evapotranspiration (ET), stem water potential, and plant widths also were determined. The objective of Expt. 2 was to determine the amount of water retained following irrigation and drainage for four pre-irrigation substrate water contents (230%, 208%, 185%, 162%; mass basis) on gravel or plastic bed surfaces. Containers on plastic or gravel beds were irrigated, drained for 1 hour, and the amount of water retained in the container substrate was determined. In Expt. 1, plastic bed surface temperatures (0730 to 1930 hr) were higher than for gravel. Container substrate temperatures on plastic were 1°C higher than gravel from 2300 to 0400 hr with no temperature differences from 0500 to 2300 hr. There were no treatment differences for other characteristics. In Expt. 2, containers on plastic retained 21%, 15%, 23%, and 16% more water than on gravel for the 230%, 208%, 185%, 162% pre-irrigation water content treatments, respectively. When containers are seated on plastic, the bottom drainage hole is sealed resulting in more water retention compared to gravel.