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from simple sequence repeat (SSR), which amplifies specifically the region between two microsatellite motifs ( Zietkiewicz et al., 1994 ). Compared with random amplified polymorphic DNA (RAPD), ISSR produces more reliable and reproducible result

for genetic diversity of palmyrah ( Borassus flabellifer ) accessions using Inter Simple Sequence Repeat (ISSR) markers Asian Austral. J. Plant Sci. Biotechnol. 3 1 11 15 Whankaew, S. Kanjanawattanawong, S. Phumichai, C. Smith, D.R. Narangajavana, J

limited number of simple sequence repeats (SSRs) are reported for Rubus , which include the invasive weed R. alceifolius ( Amsellem et al., 2001 ), an Azorean island endemic species R. hochstetterorum ( Lopes et al., 2006 ), and red raspberry ( Graham

markers ( Grati Kamoun et al., 2006 ; Taamalli et al., 2006 ) and simple sequence repeat (SSR) markers ( Taamalli et al., 2006 , 2007 ). However, there is still a need for better genetic diversity assessment and varietal identification using high

accessions. z Table 3. List of 29 additional simple sequence repeat primers screened for polymorphism in eight Rheum ×rhabarum accessions ‘Crimson Delicious’ (RB7), ‘Coulter MacDonald’ (RB10), Penn. State 3 (RB12), ‘New Zealand’ (RB19), ‘Parson’s Crimson

genotype, screening was performed on 92 olive accessions by using five high polymorphic simple sequence repeat (SSR) markers (ssrOeUA-DCA3,ssrOeUA-DCA9, ssrOeUA-DCA16, ssrOeUA-DCA18, and UDO99-043), which have been shown to be efficient for cultivar

574, and each F 1 progeny to the sample and confirming the unknown Gap1 (G 1 ) peak was between the control G 1 peaks. Simple sequence repeat generation. DNA was extracted from 118 F 1 field-grown individuals using a PureLink DNA Purification Kit

), yellow ( iiC-llR-, iiC-L-rr , and iiC-llrr ), and light-red to red ( iiC-L-R -) bulb colors, as well as black ( B -) versus brown ( bb ) seeds ( Davis, 1966 ). We scored segregations for simple sequence repeats (SSRs), bulb and seed colors, and single

accessions in the USDA collection. Materials and Methods Plant materials and simple sequence repeat markers. Thirty-six C. metuliferus accessions, six cucumber, melon, watermelon, and squash ( Cucurbita pepo ) cultivars or breeding lines, were

reproducible products were taken into account for further data analysis. Table 2. Name, motif repeat, and sequence of 33 simple sequence repeat primers used for genotyping 30 Iraqi date palm cultivars. Simple sequence repeat genotyping. Electropherogram data