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Xin Hao, Yu Fu, Wei Zhao, Lifei Liu, Rengui Bade, Agula Hasi, and Jinfeng Hao

. The reactions were performed with a Chromo 4 Real-Time PCR Detector (Bio-Rad, Hercules, CA). The relative expression of genes was calculated by the 2 (−ΔΔCt) method. At least three technical replicates were performed for each reaction and three

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Jiuxing Lu, Weiru Yang, and Qixiang Zhang

manufacturer. First-strand cDNA was synthesized from 2 μg total RNA using the TIANScript Frist Strand cDNA Synthesis Kit (Tiangen), according to the instruction of the manufacturer. Quantitative PCR was performed using the PikoReal real-time PCR system (Thermo

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Xiaoying Dou, Jinrong Bai, Huan Wang, Ying Kong, Lixin Lang, Fang Bao, and Hongzhong Shang

Synthesis Kit and an anchored-oligo (dT) 18 primer. The reaction product thus obtained was then directly used as a template for reverse-transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR amplification. RT-PCR was performed using

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Yushu Li, Zongda Xu, Weiru Yang, Tangren Cheng, Jia Wang, and Qixiang Zhang

, Tokyo, Japan). Real-time quantitative reverse transcription-PCR. The tissue-specific and developmental expression patterns of PmSOC1-1 , PmSOC1-2 , and PmSOC1-3 , were investigated by real-time quantitative reverse transcription PCR (qRT-PCR). For the

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Zhiyong Hu, Qing Liu, Meilian Tan, Hualin Yi, and Xiuxin Deng

transcriptase–polymerase chain reaction of a triploid citrus hybrid and its parents. Real-time polymerase chain reaction (PCR) was performed using the ABI 7500 Real Time System (Applied Biosystems). Actin was amplified along with the target gene as the

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E.W. Stover and T.G. McCollum

estimate disease spread due to catastrophic weather events Plant Health Progress (August):1–12. 21 Apr. 2011. < http://www.plantmanagementnetwork.org/sub/php/research/2006/canker/ >. Li, W. Hartung, J.S. Levy, L. 2006 Quantitative real-time PCR for

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Bin Peng, Jianlan Xu, Zhixiang Cai, Binbin Zhang, Mingliang Yu, and Ruijuan Ma

expression, and real-time quantitative PCR (qPCR). Sequence analysis. Intron–exon structures of the AAT genes (Prupe.5G018100, Prupe.5G018200, Prupe.5G017800, Prupe.5G017900, Prupe.5G018000) were performed following the methods of Xue et al. (2012 ). The

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Huiying Li, Hongji Luo, Deying Li, Tao Hu, and Jinmin Fu

( Srivastava et al., 2007 ). Using quantitative real-time polymerase chain reaction (Q-PCR) technique, Brunet et al. (2009) detected the increased expression of GR, APX, and glutathione S-transferase gene in the roots of grass pea ( Lathyrus sativus ) exposed

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Hiroyuki Usuda, Daisuke Nei, Yasuhiro Ito, Nobutaka Nakamura, Yutaka Ishikawa, Hitomi Umehara, Poritosh Roy, Hiroshi Okadome, Manasikan Thammawong, Takeo Shiina, Mamiko Kitagawa, and Takaaki Satake

, Le-ACS4 , and Le-ACS6 ) accumulation and ethylene biosynthesis caused by the dropping of a whole tomato fruit. To this end, Le-ACS accumulation levels were quantified by real-time polymerase chain reaction (PCR) analysis according to the distance

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Wen-hui Li, Jian-rong Feng, Shi-kui Zhang, and Zhang-hu Tang

RNA were isolated as three biological replicates for separate cDNA synthesis. The primer sets used for real-time transcriptase polymerase chain reaction (PCR) analysis were designed by Primer3 ( http://primer3.ut.ee ). Pear actin was used as internal