marker techniques, amplified fragment length polymorphism (AFLP) is a novel polymerase chain reaction (PC)R-based assay for DNA fingerprinting and polymorphism detection ( Vos et al., 1995 ). The advantages of this technique include reproducibility, high
Jinggui Fang, Jianjun Chen, Richard J. Henny, and Chih-Cheng T. Chao
Neil O. Anderson, Adnan Younis, and Ye Sun
polymorphism due to variability in the number of tandem repeats present in these sequences ( Levinson and Gutman, 1987 ). ISSR fingerprinting is more reproducible than RAPD amplification due to the longer SSR-based primers, thus enabling higher-stringency DNA
Jonathan Magby, Gayle M. Volk, Adam Henk, and Steve Miller
. Apple cultivars have been reliably identified using genetic fingerprinting techniques (microsatellites) because they have a high level of polymorphism, are codominant, and are inherited in a Mendelian fashion ( Wünsch and Hormaza, 2001 ). The goal of
Karen R. Harris-Shultz, Susana Milla-Lewis, Aaron J. Patton, Kevin Kenworthy, Ambika Chandra, F. Clint Waltz, George L. Hodnett, and David M. Stelly
using 80 SSR markers despite diverse reported collection locations ( Harris-Shultz et al., 2013 ). From the dendrogram, it was immediately apparent that samples Meyer2, Meyer3, Empire11, and Emerald2 did not match the fingerprint of the reference
Gehendra Bhattarai and Shawn A. Mehlenbacher
’, which is grown as ‘Kentish Cob’ in southeastern England. ‘Pellicule Rouge’, obtained from France, has leaves and pellicles with anthocyanin, but all other traits and its DNA fingerprint are identical to the English cultivar White Filbert ( Gökirmak et al
Yuan Yu, Chunxian Chen, Ming Huang, Qibin Yu, Dongliang Du, Matthew R. Mattia, and Frederick G. Gmitter Jr.
of Agriculture and Consumer Services) in Winter Haven, FL, using the previously used 1536-SNP genotyping assay ( Yu et al., 2016 , 2017 , 2018 ), and to determine their SNP fingerprints and to assess genetic diversity, population structure, and
A.M. Torres, T. Millán, and J.I. Cubero
Five rose (Rosa spp.) cultivars were analyzed using random amplified polymorphic DNA (RAPD) markers. Using eight primers, all cultivars were distinguished by comparing differences in DNA banding patterns. The RAPD technique fingerprints rose cultivars rapidly and inexpensively for identification and patent protection purposes.
Feiga Gutman, Avinoam Nerd, Yosef Mizrahi, Dudy Bar-Zvi, and Dina Raveh
Twenty-four genotypes of marula (Sclerocarya birrea subsp. caffra) were characterized using randomly amplified polymorphic DNA (RAPD) analysis. A distinct band pattern was obtained for each of the trees, using as few as four arbitrary 10-mer primers. Trees propagated vegetatively by grafting showed identical fingerprints. These results suggest that RAPD markers provide a useful system for documenting the identity of marula genotypes.
N. Xiang, Y. Hong, and L.T. Lam-Chan
Intensive breeding activities of tropical orchids have given rise to many hybrids, among which genetic relationships are difficult to evaluate due to free interbreeding of different species in the same genus or even from different genera, the use of hybrids for further breeding, use of abbreviated or trade names and sometimes intentional non-disclosure of parentage for commercial considerations. We have subjected 43 popular commercial Dendrobium hybrids to fluorescence amplified length polymorphism (AFLP) analysis and their genetic relationship was estimated. The hybrids bearing flowers of similar shapes and colors were clustered into five groups. Each hybrid tested had a distinct AFLP fingerprint profile except the tissue culture mutants. Sibling hybrids were closely clustered (with genetic distance <0.09) followed by those sharing one parent. These results suggest that AFLP fingerprint profiling gives accurate and objective estimation of genetic relationship of the Dendrobium hybrids tested. Our study also found that the AFLP fingerprint profiles were uniform in different parts of tested plants, stable among individuals in vegetatively propagated populations throughout different growth periods. We conclude that AFLP fingerprint profiling has the potential to be an integral part of current new plant varieties protection sytems.
Peter Boches, Nahla V. Bassil, and Lisa Rowland
Sixty-nine accessions representing wild and domesticated highbush blueberry (Vaccinium corymbosum L.) germplasm were genotyped using 28 simple sequence repeats (SSRs). A total of 627 alleles was detected and unique fingerprints were generated for all accessions. Suspected duplicate accessions of `Coville' and `Ivanhoe' had DNA fingerprints that were identical to `Coville' and `Ivanhoe', respectively. Genetic similarity measures placed wild and cultivated blueberries in separate groups. Northern highbush blueberries grouped among ancestral clones that were used extensively in blueberry breeding such as `Rubel' and `Stanley'. Southern highbush blueberries formed a separate group from northern highbush blueberries. The microsatellite markers used here show excellent promise for further use in germplasm identification, in genetic studies of wild Vaccinium L. populations, and for constructing linkage maps.