Root hydraulic conductivity (Lp) and osmotic potential (π) were measured in young, drought-stressed and non-stressed peach (Prunus persica), Olive (Olea europea), Citrumelo (Citrus paradisi x Poncirus trifoliata) and Pistachio (Pistachia integerrima) plants. Drought stress reduced Lp 2.5 to 4.2-fold, depending on species, but π was reduced only in expanded citrumelo leaves and unexpanded olive leaves by 0.34 and 1.4 MPa, respectively. A simulation model of plant water uptake and leaf water relations was constructed to quantify the offsetting effects of reduced Lp and osmotic adjustment (OA) on turgor maintenance. For olive data, a 2.5-fold reduction of Lp caused a linear decrease in turgor pressure difference between stressed and non-stressed plants, such that the effect of OA was totally offset at a leaf water potential (stressed) of ≈ -3.0 MPa. For citrumelo, because the degree of OA was lower, the water potential at which the effects of OA and reduced Lp were offsetting with respect to turgor maintenance was ≈ -0.6 MPa. The analysis suggests that some level of stomatal closure would be necessary to extend the water potential range over which stressed plants maintain higher turgor than non-stressed plants for citrumelo. Conversely, no degree of stomatal closure would be required of stressed olive plants to maintain higher turgor than non-stressed counterparts over a physiologically meaningful range of leaf water potential.
Tara Auxt Baugher, Kendall C. Elliott, and D. Michael Glenn
Three growth suppression treatments were compared during 1991 to 1993 on `Stayman' apple (Malus domestica Borkh.) trees grown in the T-trellis and the MIA trellis systems. All treatments—root pruning, K-31 fescue (Festuca arundinacea Schreb.), and K-31 fescue plus root pruning—suppressed tree growth compared to the nontreated control, but results were inconsistent between years and systems. Sod or sod plus root pruning reduced terminal shoot length in both systems in 2 out of 3 years. Root pruning decreased shoot length in the T-trellis in 1992. Sod decreased trunk cross-sectional area in the T-trellis in 1993. Treatments did not affect 3-year average yield efficiency but did appear to increase biennial bearing. Sod, with or without root pruning, decreased fruit cracking in the T-trellis 69% and 42%, respectively, in 1992, and sod plus root pruning decreased cracking in the MIA trellis 50%. Sod reduced fruit diameter in the T-trellis in 1992. Secondary effects of growth suppression treatments included increased light penetration and improved fruit color. Sod decreased leaf N and Mg and increased leaf P, K, and Cu. The Oct. 1993 stem water potential gradient from root to canopy was more negative in the sod plus root pruning treatment, and the osmotic potential of rootsucker leaves in the combination treatment was greater than in the control, indicating that sod plus root pruning alters the distribution of water within a fruit tree.
Michael V. Mickelbart and Thomas E. Marler
Sapodilla [Manilkara zapota (L.) Royen], reportedly tolerant of saline conditions relative to other tropical fruit species, was studied in sand culture under greenhouse conditions to examine the physiology of sapodilla trees exposed to NaCl and to aid in determining the basis for this apparent tolerance. Treatments, consisting of a complete nutrient solution of 1 dS·m–1 (control) or this solution amended to 12 or 20 dS·m–1 with NaCl, were administered from 16 Nov. 1991 until 29 Jan. 1992. Net CO2 assimilation (A) of plants receiving NaCl gradually decreased relative to that of the control plants. At the end of 8 weeks of salinity, A of plants receiving 12 or 20 dS·m–1 was 72% or 31% of control plants, respectively. Substrate NaCl reduced apparent quantum yield, photosynthetic CO2-use efficiency, leaf osmotic potential, and predawn xylem potential of sapodilla leaves. Dark respiration and the variable: maximal chlorophyll fluorescence ratio were not influenced by NaCl. Exposure to NaCl also increased leaf tissue Na+ and Cl– concentrations and the Na+: K+ ratio. These results indicate that gas exchange of sapodilla is relatively low for woody evergreen species. Moreover, sapodilla may not be as tolerant of salt stress as previously reported. The responses of sapodilla to root zone NaCl were consistent with other woody perennial glycophyte species. Photochemical efficiency of leaves on plants receiving NaCl was not different from that of leaves on control plants for >8 weeks after NaCl reduced gas exchange.
Y.L. Qian and J.D. Fry
`Meyer' zoysiagrass (Zoysia japonica Steud.) was established on a silt loam soil in 27-cm-diameter × 92-cm-deep containers in a greenhouse to investigate the influence of irrigation frequency on turfgrass rooting and drought tolerance. Turf was irrigated daily or at the onset of leaf rolling with a water volume equal to the cumulative evapotranspiration of well-watered turf in small weighing lysimeters. After >90 days of irrigation treatments, a dry-down was imposed during which no additional water was applied for 55 days. A recovery period followed during which time turf was watered to maintain soil matric potential at greater than –30 kPa. Compared to turf irrigated daily, that watered at the onset of leaf rolling exhibited 1) 32% to 36% lower leaf water potential and 14% to 22% lower osmotic potential before the onset of drought; 2) 13% higher leaf water potential ≈40 days into dry-down; 3) more extensive rooting at 55- and 75-cm soil depths as indicated by 11% to 19% lower volumetric soil moisture content at the end of dry-down; 4) 25% to 40% lower shoot growth rate during irrigation and 13% to 33% higher shoot growth rate during dry-down; and 5) higher quality ratings during dry-down and recovery. Thus, deep, infrequent irrigation better prepares zoysiagrass for an oncoming drought than light, frequent irrigation.
J.E. Warren and M.A. Bennett
Osmopriming has been shown to enhance seed performance by increasing germination rates and uniformity. Furthermore, these enhancements persist under less-than-optimum conditions, such as salinity, reduced water availability, and excessively high or low temperatures. Additional benefits include resistance to soil pathogens due to lower leachate levels and more rapid emergence. To augment these existing qualities, it would be advantageous to incorporate beneficial organisms that antagonize soil-borne diseases, combining the benefits of both systems into a single procedure. To accomplish this, processing tomato seeds (Lycopersicon esculentum Mill. OH8245) were bioprimed in aerated –0.8 Mpa NaNO3 at 20°C for 4 days, at which time a mixture of nutrient broth, a defoaming agent, and beneficial bacteria that has been adjusted to the same osmotic potential is added. The bacteria used, Pseudomonas aureofaciens AB254, has been proved to control Pythium ultimum on a variety of crop seeds. After 7 days the seeds are removed having been primed and colonized with 105 colony forming units (cfu)/seed. In the absence of pathogen pressure, osmoprimed and bio-osmoprimed seeds performed similarly improving overall germination by 40% after 3 days, as well as low temperature (10–15°C) germination. However, when these seeds were sown in soilless media inoculated with P. ultimum, osmoprimed and bio-osmoprimed emergence was 57% and 74%, respectively, showing the improvements that these biologicals can provide. Thermogradient table results, storage tests, cfu/seed, and pathogen control will be discussed.
Marc van Iersel
Transplanting can result in root damage, thereby limiting the uptake of water and nutrients by plants. This can slow growth and sometimes cause plant death. Antitranspirants have been used to minimize transplant shock of vegetables. The objective of this research was to determine if antitranspirants are useful to reduce transplant shock of impatiens (Impatiens wallerana Hook.f.) seedlings in the greenhouse. Seedling foliage was dipped in or sprayed with antitranspirant (Vapor Gard or WiltPruf) and shoot dry mass was determined at weekly intervals. Antitranspirants reduced posttransplant growth of impatiens as compared to untreated plants, possibly because of a decrease in stomatal conductance, leading to a decrease in photosynthesis. The two dip treatments also caused phytotoxic effects (necrotic spots) on the leaves. In a second study, leaf water, osmotic and pressure potential were determined at 2, 9, and 16 days after transplant. Application of antitranspirants (as a dip or spray) decreased water and osmotic potential compared to control plants. The results of this study indicate that antitranspirants are not useful for minimizing transplant shock of impatiens under greenhouse conditions.
Thomas E. Marler and Louann C. Guzman
Intsia bijuga is an important native tree on Guam, and is being promoted as an urban forestry tree. Container-grown I. bijuga trees were subjected to a drying cycle (50% of mean water loss replaced daily) to determine physiological responses to drought. Early to mid-morning gas exchange began to decline compared to well-watered plants on day 12, and quickly declined thereafter. Net CO2 assimilation (A) was close to zero by day 29 and became negative by day 36. Chlorophyll fluorescence of drought-stressed trees was not different from that of well-watered trees on numerous days of measurement throughout the drying cycle. Unlike leaflets of well-watered trees, leaflets of the stressed trees exhibited heliotropic movement to avoid direct exposure to the sun. The stressed trees did not respond with any change in osmotic potential of leaflets following rehydration. Carbon dioxide-use efficiency of stressed trees was reduced to 66% of well-watered trees. The most profound response following rewatering (day 37) was leaf shedding. All trees shed some leaves, and 33% of the trees shed the entire canopy. On the trees that retained some leaves, A returned to that of the control trees by day 13 of recovery.
L. Phavaphutanon, F.T. Davies Jr., T.W. Boutton, and S.A. Duray
Mycorrhizal (VAM) and phosphorus (P)-supplemented nonmycorrhizal neem plants (non-VAM) of comparable size and tissue nutrition were subjected to a slowly developing drought. VAM and non-VAM plants responded to drought similarly. However, mycorrhiza compensated for low P supply, allowing VAM plants to have comparable growth, tissue P, and other physiological parameters as non-VAM plants, which received higher P supply. Drought decreased growth, transpiration (E), photosynthetic rate (A), stomatal conductance (gs), and plant water status. Osmotic adjustment did not occur, but the relatively low osmotic potential of this species helped maintain turgor during drought. Plant water relations and A of stressed plants fully recovered in 24 hours after rehydration, while gs and E partially recovered. Instantaneous water use efficiency (A/E) increased during drought and recovery, except for a decrease at peak stress due to very low A. Carbon isotope discrimination (D) values of mature leaves remained constant regardless of mycorrhiza or drought. However, D decreased in expanding leaves that developed during a drought period, indicating an increased long-term water use efficiency of these leaves.
N. Wartidiningsih, R.L. Geneve, and S.T. Kester
High germination seed lots of purple coneflower [Echinacea purpurea (L.) Moench] were evaluated for laboratory germination following osmotic priming or chilling stratification. Compared to nontreated seeds, osmotic priming at 25C in salts (KNO3 + K3PO4; 1:1, w/w) or polyethylene glycol 4000 (PEG) increased early (3-day) germination percentage at 27C of all seed lots, and improved total (10-day) germination percentage of low-germination seed lots. Total germination percentage was unaffected or increased by priming for 4 days compared to 8 days, and by priming at –1.0 MPa compared to –0.5 MPa (except for one low-germination seed lot). Chilling stratification in water at 5 or 10C increased early and total germination of all seed lots, except for that same lot, compared to nontreated seeds. Total germination percentage was unaffected or increased by stratification at 10C rather than at 5C. Neither extending stratification ≥20 days nor lowering osmotic potential with PEG during stratification improved total germination percentage.
Thomas G. Ranney, R.E. Bir, and W.A. Skroch
In order to evaluate and compare adaptability to dry sites, plant water relations and leaf gas exchange were compared in response to water stress among six birch species: monarch birch (Betula maximowicziana), river birch (B. nigra), paper birch (B. papyrifera), European birch (B. pendula), `Whitespire' Japanese birch (B. platyphylla var. japonica `Whitespire'), and gray birch (B. pendula). After 28 days without irrigation, Japanese birch maintained significantly higher stomatal conductance (gs) and net photosynthesis (Pn) than did any of the other species, despite having one of the lowest mid-day water potentials. Evaluation of tissue water relations, using pressure-volume methodology, showed no evidence of osmotic adjustment for any of these species in response to water stress. However, there was substantial variation among species in the water potential at the turgor loss point; varying from a high of -1.34 MPa for river birch to a low of -1.78 MPa for Japanese birch. Rates of Pn and gs under mild stress (mean predawn leaf water potential of -0.61 MPa) were negatively correlated with leaf osmotic potential at full turgor and the leaf water potential at the turgor loss point.