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Jose Linares, Johannes Scholberg, Kenneth Boote, Carlene A. Chase, James J. Ferguson, and Robert McSorley

Morra, M.J. Kirkegaard, J.A. 2002 Isothiocyanate release from soil-incorporated brassica tissues Soil Biol. Biochem. 34 1683 1690 Muir, J. 2002 Hand-plucked forage yield and quality and seed production

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Yan Li, Hongyan Qi, Yazhong Jin, Xiaobin Tian, Linlin Sui, and Yan Qiu

) and Tian et al. (2012) . Peel and flesh tissues (3 g each) were ground in liquid nitrogen using a baked mortar and pestle. The ground powder was transferred to 50-mL polypropylene tubes with 15 mL of guanidine isothiocyanate extraction buffer that had

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Jinyu Wang, Bo Yuan, Yi Xu, and Bingru Huang

Physiol. 136 4159 4168 Khan, J.K. Kuo, Y-H. Kebede, N. Lambein, F. 1994 Determination of non-protein amino acids and toxins in Lathyrus by high-performance liquid chromatography with precolumn phenyl isothiocyanate derivatization J. Chromatography 687

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Shuguang Wang, Yongpeng Ma, Chengbin Wan, Chungyun Hse, Todd F. Shupe, Yujun Wang, and Changming Wang

were incubated with fluorescein isothiocyanate–labeled goat antimouse IgG (Sigma-Aldrich) diluted 1:50 in blocking solution in the dark for 45 min at room temperature. The sections were rinsed with TBST② (three 3-min washes), and examined under a

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Anthony M. Ortiz, Brent S. Sipes, Susan C. Miyasaka, and Alton S. Arakaki

when tissues are disrupted, forming isothiocyanates that are nematicidal ( Chitwood, 2002 ; McLeod and Steel, 1999 ; Oka, 2010 ). Aqueous extracts of Crotalaria species were reported to protect tomato ( Solanum lycopersicum L.) roots from

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T. Casey Barickman, Dean A. Kopsell, and Carl E. Sams

.C. Kopsell, D.A. Sams, C. 2013 Selenium influences glucosinolate and isothiocyanates and increases sulfur uptake in Arabidopsis thaliana and rapid-cycling Brassica oleracea J. Agr. Food Chem. 61 202 209 Barickman, T.C. Kopsell, D.A. Sams, C. 2014a Abscisic

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Kellie J. Walters, Bridget K. Behe, Christopher J. Currey, and Roberto G. Lopez

water HortTechnology 7 49 55 Bell, L. Oloyede, O.O. Lignou, S. Wagstaff, C. Methven, L. 2018 Taste and flavor perceptions of glucosinolates, isothiocyanates, and related compounds Mol. Nutr. Food Res. 62 18 1700990 Benard, C. Gautier, H. Bourgaud, F