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Xuefei Ning, Xianlei Wang, Zhijie Yu, Simeng Lu, and Guan Li

) with minor modifications. The purified DNA was diluted to 100 ng·μL −1 . A total of 499 SSR markers were selected from published sources ( Chiba et al., 2003 ; Danin-Poleg et al., 2000 ; Diaz et al., 2011 , 2015 ; Fernandezsilva et al., 2008

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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe, and Sheri B. Crabtree

selections using SSR markers. Materials and Methods Plant material. Leaf samples were collected from older and new (PPF) pawpaw cultivars and KSU advanced selections ( Table 1 ). Leaf samples were collected in Spring 2009 and 2010 from pawpaw trees located at

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Mohammad Sadat Hosseini Grouh, Kourosh Vahdati, Mahmoud Lotfi, Darab Hassani, and Nejat Pirvali Biranvand

-2-phenylindole was added for DNA staining. After 2 min incubation, nuclei were passed through a 30-μm nylon filter to eliminate cell debris. The samples were analyzed using a flow cytometer (PA-I; Partec). Molecular characterization. SSR markers

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Yan-Chang Wang, Lei Zhang, Yu-Ping Man, Zuo-Zhou Li, and Rui Qin

-fleshed kiwifruit or further evaluate the commercial potential. In addition, novel variations can be derived from the budsports of ‘Hongyang’ and improper introduction. SSR markers were used to characterize genetic variability and confirm true-to-type identity of

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Yanbin Su, Yumei Liu, Huolin Shen, Xingguo Xiao, Zhansheng Li, Zhiyuan Fang, Limei Yang, Mu Zhuang, and Yangyong Zhang

x cabbage cross and used it in a segregation analysis over multiple generations (P 1 , P 2 , and DHs) to explain HSR inheritance. We then constructed a framework linkage map based on this DH population with SSR markers. Finally, we located the first

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Karen Harris-Shultz, Melanie Harrison, Phillip A. Wadl, Robert N. Trigiano, and Timothy Rinehart

). Simple sequence repeat markers, or microsatellites, are repeating DNA sequences of one to six nucleotides that are found in coding and non-coding regions of the genome ( Toth et al., 2000 ). SSR markers, including expressed sequence tag markers, have a

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Barbara Gilmore, Nahla Bassil, April Nyberg, Brian Knaus, Don Smith, Danny L. Barney, and Kim Hummer

amounts of sequence data, but require specialized and devoted computer infrastructure and bioinformatics ( Cronn et al., 2008 ). The resulting sequence data can be applied to the development of SSR markers in species that lack or have few available SSRs

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Xinwang Wang, Phillip A. Wadl, Cecil Pounders, Robert N. Trigiano, Raul I. Cabrera, Brian E. Scheffler, Margaret Pooler, and Timothy A. Rinehart

, allele sizes documented here suggest that there may be an increased frequency of different alleles on homologous chromosome. Linking SSR markers with important traits may be possible because the same parents are used through multiple generations and they

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Davut Keleş, Hasan Pınar, Atilla Ata, Hatıra Taşkın, Serhat Yıldız, and Saadet Büyükalaca

) haploid and ( B ) diploid plants. SSR analyses. Embryo type (diploid/doubled haploid) was determined using Hpms1-117 SSR marker ( Fig. 4 ). More than 20 SSR markers derived from the pepper genome (Sol Genom network) were tested in this study and Hpms1

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Yiqun Weng

squash line ( Table 1 ). The DNA sample of ‘New Hampshire Midget’ was provided by Amnon Levi at the USDA-ARS in Charleston, SC. Table 1. Plant materials used in this study. All simple sequence repeat (SSR) markers were developed from the