Phalaenopsis (Phalaenopsis spp.) is the most important indoor potted plant worldwide. Tissue analysis is very important for managing fertilization practices but the effects of sampling position and plant maturity must be considered. However, there has been little research on the distribution of tissue carbon (C) and nitrogen (N) among leaves and changes of tissue C and N composition during various developmental stages in phalaenopsis. In this study, we thus determined the effects of leaf age, plant maturity, and cultivars on C and N partitioning in phalaenopsis. Overall, C concentration was more uniform and was less affected by the abovementioned factors investigated, whereas N concentration significantly decreased as leaves aged or as plants matured. In P. Sogo Yukidian ‘V3’, new expanding leaf had the highest N concentration of 2.72% of dry weight (DW) and seventh mature leaf had the lowest value of 1.48% DW. Results also indicate that N was not evenly distributed within a leaf, whereas N concentration gradually decreased from the leaf tip to the leaf base. The middle section of the second mature leaf is an appropriate tissue for sampling to obtain the representative N and C concentrations in phalaenopsis. As for the changes in C and N composition through five developmental stages, two cultivars were compared, including the large, white-flowered P. Sogo Yukidian ‘V3’ and the small, purple-flowered P. Sogo Lotte ‘F2510’. As the large-flowered ‘V3’ grew from deflasked plantlet to fully matured plant (18 months after deflasking) in a 10.5-cm pot, whole-plant N concentration decreased from 4.63% DW to 1.67% DW and C/N thus increased from 9.1 to 26.1. Despite the large difference in plant size, the small-flowered ‘F2510’ had a similar trend and values during vegetative growth stages. However, the two cultivars had different trends during reproductive stages. Tissue N concentration and C/N did not further change as mature large-flowered ‘V3’ plants were forced to flower. By contrast, tissue N concentration in small-flowered ‘F2510’ further decreased and C/N thus further increased, which was due to its small stored N pool. Major N sink organ shifted from roots to inflorescences during reproductive growth and the stored N in roots as well as in leaves was then used for flower development.
Jen-An Lin and Yao-Chien Alex Chang
Ya-Ching Chuang and Yao-Chien Alex Chang
The vase life of Eustoma cut flowers can be extended by adding sugars to the vase solution, but the exact role of sugars and how they are translocated in tissues are not clear. Thus, we observed the preserving effect of different sugars in vase solutions on Eustoma and compared sugar concentrations in vase solutions and in the flowers as well as stems and leaves of cut flowers in a solution containing 200 mg·L−1 8-hydroxyquinoline sulfate (8-HQS) with and without 20 g·L−1 sucrose during different flowering stages. Inclusion of glucose, fructose, or sucrose in the vase solution extended the vase life of cut flowers with no significant differences among sugar types. During flower opening, the concentration of added sucrose in the vase solution dropped, and the fresh weight (FW), glucose concentration, and sucrose concentration of flowers in sucrose solutions increased, whereas flowers in solutions without sucrose had lower FW and glucose concentrations. During flower senescence, sugar concentration in the vase solution did not change much, but the FW and sucrose concentrations in all flowers declined, although the FW of sucrose-treated flowers fell more slowly. For stems and leaves in the sucrose solution, sugar concentrations increased during the first 7 days with only glucose slightly declining during senescence, whereas the FW was maintained during the entire vase life. In contrast, FWs of those in the solution without sucrose gradually declined. In conclusion, sucrose in the vase solution promoted flower opening and maintained the water balance of Eustoma cut flowers. Glucose and fructose also extended the vase life, likely in similar ways.
Yin-Tung Wang and Yao-Chien Alex Chang
Growers realize the importance of nitrogen (N) on the vegetative growth of phalaenopsis orchids (hybrids of Phalaenopsis sp.), but often overlook its influence on reproductive growth. Low N may result in slow plant growth, pale-green leaves, abscission of lower leaves, and few flowers in phalaenopsis. Increasing N concentration up to 200 mg·L−1 promotes leaf growth and increases flower count. High N concentration promotes lateral branching on the flowering stalk, thereby greatly increasing the total flower count and elevating the commercial value. It is important that N be continually applied during the forcing period for best flowering performance, particularly for those that had undergone international shipping. For the vegetative phalaenopsis plants that are induced to flower without being shipped internationally, the N that is already in the plant before spiking provides 43% and the N being absorbed by roots after cooling provides 57% of the total N in the inflorescence at time of visible bud. When insufficiently fertilized or no fertilization is applied during the forcing period, more of the existing N in a plant is mobilized for inflorescence development. Phalaenopsis roots can take up all three forms of N [i.e., nitrate (NO3-N), ammonium (NH4-N), and urea] directly. In two studies, phalaenopsis plants were supplied with the same amount of total N but with varying NO3-N from 100%, 75%, 50%, 25%, to 0% (a common N concentration was achieved by the substitution of the respective balance with NH4-N). Plants were smaller when receiving 75% or 100% NH4-N with a tendency of decreasing top leaf width and whole-plant leaf spread as NO3-N decreased from 100% to 0%. Spiking was delayed and spiking rate decreased when plants were grown in sphagnum moss, but not a bark mix, and received more than 50% of the N in NH4-N. As the ratio between NO3-N and NH4-N increased, flowers became increasingly larger. The negative effects of low ratios of NO3-N to NH4-N were more severe in the second flowering cycle. When supplied with 50% or more NH4-N, the absorption of cations by phalaenopsis roots declined, with reduced concentrations of calcium and magnesium in plants, while symptoms of ammonium toxicity appeared, including growth retardation, chlorotic leaves, and necrotic roots. In conclusion, adequate N and its continual supply during both vegetative and reproductive stages are recommended for the best growth and flowering of phalaenopsis. Since phalaenopsis plants prefer N in the NO3-N form, it is suggested that growers choose and apply a fertilizer with nitrate as the major N source.
Hadi Susilo and Yao-Chien Alex Chang
Plants of Phalaenopsis orchid are known for their great resilience and ability to flower under less than ideal conditions, including long periods without fertilization. Significant nutrient storage is thought to account for this characteristic; however, the use of stored nutrients in Phalaenopsis has not been fully studied. We used 15N-labeled Johnson’s solution to trace the use of stored nitrogen (N) and recently absorbed fertilizer N in Phalaenopsis given various fertilizer levels during forcing. By separately labeling fertilizer N applied to Phalaenopsis Sogo Yukidian ‘V3’ plants 6 weeks before and 6 weeks into forcing, we found in the inflorescence that the ratio of N derived from fertilizer applied 6 weeks before forcing to the N derived from fertilizer applied 6 weeks into forcing was 43% to 57%. With 90% reduction in fertilizer concentration during the reproductive stage, the ratio increased to 89% to 11%, indicating that stored N becomes a significant N source for inflorescence development when fertility becomes limited. Reducing fertilizer level during the reproductive stage from full-strength Johnson’s solution down to zero decreased the dry weight of newly grown leaves, reduced the number of flowers from 10.8 to 8.9, and slightly increased the time required between initiation of forcing and anthesis. However, the overall effect of reduced fertilization on the growth and flowering of Phalaenopsis Sogo Yukidian ‘V3’ plants in this study was slight, because under little or no fertilization, more stored N was mobilized and this was sufficient to meet most of the N demand for inflorescence development.
Wei-Ling Guo, Yao-Chien Alex Chang, and Chien-Yuan Kao
Cyrtopodium paranaense is a tropical terrestrial orchid, which propagates mainly through sexual seed germination. In this study, we document the asexual morphogenesis of the root tip to protocorm-like body (PLB) conversion in Cyrtopodium paranaense. Protocorm-like bodies sporadically developed from root tips of flask-grown seedlings in the absence of any exogenous plant growth regulators (PGRs). The compact PLBs ultimately gave rise to normal plantlets. Histological observations revealed that the root cap became dissociated from the root apex at an early stage followed by dispersed extension of root vascular strands into nascent PLBs. Protocorm-like bodies also developed from the root central stele tissue. In root tip segment cultures, PLBs were not formed without providing PGRs but were efficiently formed from root tips in Murashige and Skoog (MS) medium supplemented with 10.2 μM indole-3-acetic acid (IAA) and 9.0 μM thidiazuron (TDZ). Both IAA and TDZ promoted the formation of PLBs; however, TDZ did not induce PLB formation in the absence of IAA, indicating a synergistic effect of the two PGRs. Protocorm-like bodies were proliferated and subsequently plants regenerated in PGR-free MS medium. Root tip culture may be used as an alternative method for the propagation of Cyrtopodium paranaense.
Ariningsun P. Cinantya, Fure-Chyi Chen, and Yao-Chien Alex Chang
The popularity of the nobile-type dendrobium (Dendrobium nobile hybrids) has been increasing globally. More information regarding the effects of long-distance shipping, from producing countries to destination market countries, on the post-shipping plant performance is needed. In this study, two nobile-type dendrobium cultivars were subjected to simulated dark shipping (SDS) at various temperatures and durations. Changes in net CO2 uptake rate (Pn), chlorophyll fluorescence, and leaf relative water content after plants had been treated with SDS were investigated. Furthermore, shipped plants were vernalized to investigate the effect of dark shipping on the subsequent flowering quality. Dark shipping for 7 days at 15 °C did not affect the post-shipping photosynthetic performance of D. Lan Tarn Beauty. Increasing the shipping duration from 7 to 21 days increased the time required for Pn recovery from 1 to 12 days. Dendrobium Lan Tarn Beauty recovered its Pn within 4 days when shipped for 21 days at 10 °C, and this was prolonged to 11 days when the plants were shipped at 20 °C. Changes in Fv/Fm indicated that there was no marked damage to either cultivars, and the leaf relative water content was little affected by SDS. Dendrobium Lan Tarn Beauty and D. Lucky Girl shipped at 10 °C flowered 5 and 8 days earlier, respectively, compared with unshipped plants. Regardless of the shipping conditions, shipped D. Lucky Girl had a lower flower diameter and higher total flower count than unshipped plants. No differences were found in the number of nodes with flowers or the total flower count between shipped and unshipped D. Lan Tarn Beauty. Our study suggested that dark shipping for up to 21 days is possible for nobile-type dendrobiums. We recommend shipping temperatures of 10 to 15 °C to reduce the detrimental effects caused by long-term dark shipping.
Wan-Yi Yen, Yao-Chien Alex Chang, and Yin-Tung Wang
Sphagnum moss has been used as the major substrate for cultivating Phalaenopsis spp. in China, Japan, and Taiwan. With a lengthened duration of cultivation, the pH of the moss gradually declines. It is not understood what causes this decline in substrate pH. Using the vegetatively propagated Phal. Sogo Yukidian ‘V3’, this study investigated if substrate, fertilization, light, and plant roots could be the cause of pH decline in the substrate. The results showed that, although increasing fertilizer concentration resulted in a low initial pH (pH measured by the pour-through technique at first fertilization), fertilization itself was not the primary cause of the long-term pH decline. Regardless of whether the sphagnum moss was fertilized, the pH of the substrate without plants increased as time progressed, whereas the pH of the substrate in which living Phalaenopsis plants were growing declined with time. Although the magnitude and course of pH decline were different in various substrates, the pH of sphagnum moss, artificial textile fiber, and pine bark substrates in which living plants were growing declined with time. Whether the substrate was exposed to light (clear pots) or not (opaque pots) had no effect on substrate pH, indicating that algae were not a factor in pH decline. Therefore, the roots of Phalaenopsis may be the major contributor to substrate pH decline during production.
Jiunn-Yan Hou, William B. Miller, and Yao-Chien Alex Chang
Phalaenopsis is one of the most important ornamental crops and is frequently transported between continents. In this study, the effects of the duration and temperature of simulated dark shipping (SDS) and the temperature difference between cultivation greenhouses and shipping containers on the carbohydrate status and post-shipping performance were investigated. With a prolonged SDS from 0 to 40 days at 20 °C, the percentage of the vegetative Phalaenopsis Sogo Yukidian ‘V3’ plants with yellowed leaves increased from 0% to 50%, and the total carbohydrate contents in the shoot and roots gradually decreased over time. Furthermore, roots had greater reductions in glucose and fructose concentrations than the shoot after 40 days of SDS. After 7 days of SDS, the youngest bud and the nearly open bud on blooming plants of Phalaenopsis amabilis were found to be the most negatively affected among flowers and buds of all stages. These buds had lower soluble sugar concentrations and flower longevities compared with those of unshipped plants. The results of a temperature experiment showed that yellowing of the leaves and chilling injury (CI) occurred in Phalaenopsis Sogo Yukidian ‘V3’ after 21 days of SDS at 25 and 15 °C, respectively, regardless of pre-shipping temperature acclimation. However, 10 days of acclimation at 25/20 °C (day/night) before SDS reduced CI and reduced the time to inflorescence emergence. Higher accumulations of sucrose in the shoot and glucose and fructose in roots were found after 21 days of SDS at 15 °C compared with those at 25 and 20 °C. In conclusion, the carbohydrate status of Phalaenopsis was positively related to the post-performance quality. A reduction in the commercial quality after SDS may be attributed to a decline in carbohydrates. The optimal temperature for long-term dark shipping is 20 °C, and we recommend providing 10 days of lower-temperature acclimation (25/20 °C) before shipping to enhance the chilling tolerance and to promote early spiking of Phalaenopsis plants.
Hadi Susilo, Ying-Chun Peng, and Yao-Chien Alex Chang
Phalaenopsis orchid is a slow-growing crop that responds slowly to fertilization. In this study, we used 15N-labeled Johnson’s solution to investigate the accumulation and use of fertilizer nitrogen (N) during the vegetative and reproductive growth stages of Phalaenopsis Sogo Yukidian ‘V3’ with a focus on the nitrogen source for inflorescence development. Labeling of fertilizer applied to mature plants 6 weeks before forcing or at 6 weeks into forcing showed that in the inflorescence, the ratio of N derived from fertilizer applied 6 weeks before forcing to the N derived from fertilizer applied 6 weeks into forcing was 31% to 69%, which shows the importance of newly absorbed fertilizer for supplying the N needed for inflorescence development. The fate of fertilizer N applied during the small, medium, or large plant stage of vegetative Phalaenopsis Sogo Yukidian ‘V3’ was traced separately with 15N-labeling. The capacity of the plant to accumulate N after fertilizer application was different during the various stages of vegetative growth, with large plants having more N storage capacity as a result of their greater biomass. However, the percentage of the accumulated N that was later allocated to the inflorescence was similar regardless of the stage of fertilizer application: of the fertilizer N absorbed during various stages of the vegetative period, 6% to 8% was allocated to the inflorescence at the visible bud stage. This result highlights the mobility of N stored early on within the plant. By calculation, of the total N in the inflorescence at the visible bud stage, the N absorbed during the small, medium, and large plant stages contributed 7%, 11%, and 25%, respectively, whereas N applied after spiking made up the other 57%. This result indicates that both N stored during the vegetative stage and N applied during the reproductive stage contribute significantly to inflorescence development.
Jiunn-Yan Hou, Tim L. Setter, and Yao-Chien Alex Chang
Phalaenopsis plants are routinely shipped long distances in total darkness. To determine how these long dark periods affect photosynthetic status in Phalaenopsis Sogo Yukidian ‘V3’, changes of net CO2 uptake, photosystem II (PS II) efficiency, and abscisic acid (ABA) concentration after a long-term simulated dark shipping were investigated. Net CO2 uptake rate, malate concentration, and titratable acidity in potted Phalaenopsis Sogo Yukidian ‘V3’ decreased after a 21-day simulated dark shipping at 20 °C, but recovered gradually with time after shipping. It took 6 to 9 days to recover to a normal photosynthetic status after shipping. The value of Fv/Fm was little affected by shipping. Therefore, net CO2 uptake rate would be a better indicator for estimating the recovery time after shipping. After shipping, fresh weight loss, leaf ABA concentration, and number of yellowed leaves of bare-root plants were higher than those of potted plants, and increased with longer durations (7, 14, and 21 days) of the simulated dark period. The spiking (the emergence of flowering stems) date was delayed when plants were stored in a bare-root condition. The concentration of ABA in leaves rose in the first 3 days after simulated shipping and then decreased within the next 3 to 8 days. Plants that received photosynthetic photon flux (PPF) at 399 μmol·m−2·s−1 after shipping had lower PS II efficiency and reduced net CO2 uptake rate than those given less PPF levels. We recommend a post-shipping acclimation for 6 to 9 days with gradual light increase (34–72–140–200 μmol·m−2·s−1 PPF) or maintaining a light level of 140 μmol·m−2·s−1 PPF for Phalaenopsis to achieve a better photosynthetic status after prolonged dark storage.