Cytological features of ‘Aijiaohuang’ chinese cabbage-pak-choi (Brassica campestris ssp. chinensis) Bcajh97-01A/B genic male-sterile AB line were examined to determine phenotypic reasons for male sterility. The sterile line Bcajh97-01A was found to undergo aberrant cytokinesis during male meiosis. Transcriptional profiling of the flower buds of both fertile and sterile plants was performed at the periods preceding meiosis, at the tetrad to uninucleate pollen period, and at the binucleate to mature pollen period. Transcript-derived fragments (TDFs) from corresponding genes that were expressed in flower buds at these three different stages could be divided into nine classes. We sequenced a total of 14 new TDFs that were differentially displayed at particular pollen developmental stages, including eight genes with unknown or hypothetical functions and six genes showing significant homology with known genes. This characterization of the Bcajh97-01A genic male-sterile line allowed the identification of candidate genes underlying genic male sterility.
Li Huang, Wan-zhi Ye, Ting-ting Liu, and Jia-shu Cao
Yue Wen, Shu-chai Su, Ting-ting Jia, and Xiang-nan Wang
The periods of flower bud differentiation and fruit growth for Camellia oleifera overlap greatly affect the allocation of photoassimilates to flower buds and fruit, resulting in obvious alternate bearing. To export the cause and mitigate alternate bearing of Camellia oleifera, the allocation of photoassimilates to buds and fruit supplied by leaves at different node positions was studied by the addition of labeled 13CO2 during the slow fruit growth stage. The fate of 13C photoassimilated carbon was followed during four periods: slow fruit growth (4 hours and 10 days after 13C labeling); rapid growth (63 days after 13C labeling); oil conversion (129 days after 13C labeling); and maturation (159 days after 13C labeling). Photosynthetic parameters and leaf areas of the leaves shared a common pattern (fifth > third > first), and the order of photosynthetic parameters of different fruit growth stages was as follows: oil conversion > maturation > rapid growth > slow growth. The most intense competition between flower bud differentiation and fruit growth occurred during the oil conversion stage. Dry matter accumulation in different sinks occurred as follow: fruit > flower bud > leaf bud. Photoassimilates from the labeled first leaf were mainly translocated to the first flower bud, and the upper buds were always differentiated into flower buds. The photoassimilates from the labeled third leaf were distributed disproportionately to the third flower bud and fruit. They distributed more to the third flower bud, and the middle buds formed either flower or leaf buds. However, the photoassimilates from the labeled fifth leaf were primarily allocated to the fruit that bore on the first node of last year’s bearing shoot, and basal buds did not form flower buds. Based on our results, the basal leaves should be retained for a high yield in the current year, and the top leaves should be retained for a high yield in the following year. Our results have important implications for understanding the management of flower and fruit in C. oleifera. The thinning of fruit during the on-crop year can promote flower bud formation and increase the yield of C. oleifera crops in the following year. During the off-year, more fruit should be retained to maintain the fruit yield. The thinning of middle-upper buds could promote more photoassimilates allocate to the fruit.