The influence of daily light integral (DLI) before vernalization and vernalization temperature and duration on growth and flower development was determined for seed-propagated perennials Aquilegia ×hybrida Sims `Remembrance', Coreopsis grandiflora Hogg ex Sweet `Sunray', and Lavandula angustifolia Mill. `Hidcote Blue'. Seedlings were grown under two DLIs (4 or 14 mol·m-2·d-l) for 5 weeks before being vernalized at -2.5, 0, 2.5, or 5 °C for 2,4,5, or 8 weeks. `Remembrance' and `Sunray' plants were vernalized in the dark, while `Hidcote Blue' plants were vernalized in light at 5 to 10 μmol·m-2·s-l for 9 hourslday. After vernalization, plants were forced under a 16-h photoperiod in the greenhouse at 20±2 °C. `Remembrance' plants flowered uniformly when vernalized at 0 to 2.5 °C for 2 weeks or longer, and flower number, plant height, time to visible bud or to flower were generally not influenced by vernalization temperature or duration. No `Sunray' plants flowered without vernalization, and only a low percentage flowered with 4-week vernalization. Compared with low DLI, a 14 mol·m-2·d-1 before vernalization delayed flowering by 7 to 20 days in `Remembrance', but there were no substantial differences in flowering characteristics of `Sunray'. `Hidcote Blue' plants were best vernalized in the light at 5 °C for 8 weeks to obtain rapid and uniform flowering and the highest number of inflorescences. Flowering and survival percentages of `Hidcote Blue' were much lower for plants at 14 mol·m-2·d-l DLI compared to 4 mol·m-2·d-1.
Genhua Niu, Royal Heins, Arthur Cameron, and William Carlson
Yoshiaki Kitaya, Genhua Niu, Toyoki Kozai, and Maki Ohashi
Lettuce (Lactuca sativa L. cv. Summer-green) plug transplants were grown for 3 weeks under 16 combinations of four levels (100, 150, 200, and 300 μmol·m-2·s-1) of photosynthetic photon flux (PPF), two photoperiods (16 and 24 h), and two levels of CO2 (400 and 800 μmol·mol-1) in growth chambers maintained at an air temperature of 20 ±2 °C. As PPF increased, dry mass (DM), percent DM, and leaf number increased, while ratio of shoot to root dry mass (S/R), ratio of leaf length to leaf width (LL/LW), specific leaf area, and hypocotyl length decreased. At the same PPF, DM was increased by 25% to 100% and 10% to 100% with extended photoperiod and elevated CO2 concentration, respectively. Dry mass, percent DM, and leaf number increased linearly with daily light integral (DLI, the product of PPF and photoperiod), while S/R, specific leaf area, LL/LW and hypocotyl length decreased as DLI increased under each CO2 concentration. Hypocotyl length was influenced by PPF and photoperiod, but not by CO2 concentration. Leaf morphology, which can be reflected by LL/LW, was substantially influenced by PPF at 100 to 200 μmol·m-2·s-1, but not at 200 to 300 μmol·m-2·s-1. At the same DLI, the longer photoperiod promoted growth under the low CO2 concentration, but not under the high CO2 concentration. Longer photoperiod and/or higher CO2 concentration compensated for a low PPF.
Joseph Masabni, Youping Sun, Genhua Niu, and Priscilla Del Valle
Southern U.S. states such as Texas experience high temperatures and intense solar radiation during the summer production season. Use of shadecloth is common in Spain and other Mediterranean countries and is becoming popular with homeowners or small-acreage farmers in Texas. Little information is available on the applicability of using shadecloth on tomato (Solanum lycopersicum) and chili pepper (Capsicum annuum) in the warm climate of Texas. The effects of two shade nets differing in shading intensity on growth, chlorophyll fluorescence, and photosynthesis of ‘Celebrity’ tomato and ‘Sweet Banana’ chili pepper was investigated from May to Aug. 2014. Plants were grown in 50% shade, 70% shade, or full sun. Compared with the unshaded control, tomato grown in 50% shade had similar yield and shoot fresh and dry weight and less photochemical stress. The 50% shade reduced number and weight of unmarketable tomato fruit. Similar results were obtained with chili pepper except for lower numbers of marketable fruit. The 70% shade significantly reduced yield parameters of both tomato and chili pepper. Both 50% and 70% shadecloth reduced leaf temperatures of tomato and chili pepper with variable results in June and July. Growth index [(height + width 1 + width 2) ÷ 3] of tomato and chili pepper was the highest with 50% shade, the lowest with full sun, and intermediate with 70% shade. The maximum net photosynthetic rates (Pn) of tomato determined from a Pn to light response curve supported the results on growth and yield. However, the maximum Pn of chili pepper was higher in full sun treatment compared with 50% or 70% shade. The latter two were almost identical. This one growing season study indicated that shading at 50% benefits tomato and chili pepper production in west Texas by reducing heat stress; however, a shading percentage below 50% may be better.
Ockert Greyvenstein, Terri Starman, Brent Pemberton, Genhua Niu, and David Byrne
The decline of garden rose sales over the past 20 years can be partially attributed to the lack of material adapted to a wide range of landscapes, which includes adaptation to high temperature stress. Current methods for evaluating high temperature susceptibility in garden roses are based on field observations, which are time consuming and subjected to ever-changing environmental conditions. A series of experiments were conducted to optimize protocols and compare the use of chlorophyll fluorescence (CFL) and cell membrane thermostability (MTS) by way of electrolyte leakage as methods to screen for high temperature susceptibility. Immature leaves proved better than mature leaves for both CFL and MTS measurements, using either detached leaf or whole plant stress assays. MTS measured on immature leaves stressed in a water bath at 50 °C for 45 minutes proved most consistent in separating rose clones based on high temperature susceptibility. Stressing actively growing plants with flower buds of 2 mm in diameter in a heat chamber at 44 °C for 3 hours resulted in increased flower abscission and leaf necrotic lesions on more susceptible clones when compared with those that were heat tolerant. Combining MTS measurements from immature leaves stressed in a water bath with the flower abscission and leaf necrosis responses 10 days after stress in a heat chamber could be the first step to screen and select against the more susceptible clones in a garden rose breeding program. Power analyses suggest that the proposed MTS protocol would be efficient in detecting differences between clones when the difference in electrolyte leakage is greater than 10%.
Shasha Wu, Youping Sun, Genhua Niu, James Altland, and Raul Cabrera
Asteraceae is one of the largest plant families with many important garden ornamental species. Salt tolerance of 10 aster perennials was evaluated in a greenhouse experiment, including the following: damianita (Chrysactinia mexicana), gregg’s mistflower (Eupatorium greggii), shasta daisy (Leucanthemum ×superbum ‘Becky’), blackfoot daisy (Melampodium leucanthum), lavender cotton (Santolina chamaecyparissus), aromatic aster (Symphyotrichum oblongifolium), copper canyon daisy (Tagetes lemmonii), four-nerve daisy (Tetraneuris scaposa), skeleton-leaf goldeneye (Viguiera stenoloba), and zexmenia (Wedelia texana). Plants were irrigated with nutrient solution at electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solutions at EC of 5.0 or 10.0 dS·m−1 (EC 5 or EC 10) for 5 weeks. Upon termination, growth parameters, foliar salt damage, relative chlorophyll content [Soil-Plant Analysis Development (SPAD) readings], and mineral concentration were measured. Gregg’s mistflower, skeleton-leaf goldeneye, and lavender cotton were the most salt-tolerant species with less reductions in shoot dry weight (DW) in both EC 5 and EC 10. Considering the relatively severe foliar salt damage (visual quality score of 3.1 and 2.7 at EC 5; 2.4 and 1.6 at EC 10) and mortality rate (10% and 40%) in EC 10, aromatic aster and zexmenia should be avoided where poor quality water may be used for irrigation. Gregg’s mistflower and skeleton-leaf goldeneye had relatively lower leaf sodium (Na) concentrations suggesting that both species can selectively exclude Na. Damianita and the four daisies, i.e., blackfoot daisy, copper canyon daisy, four-nerve daisy, and shasta daisy, were salt sensitive as evidenced by their greater growth reduction, foliar salt damage, and high Na and chlorine (Cl) accumulation in leaves, and should be avoided in landscapes where poor quality water may be used for irrigation.
Zhengnan Yan, Dongxian He, Genhua Niu, Qing Zhou, and Yinghua Qu
Few researchers examined different red light amounts added in white light-emitting diodes (LEDs) with varied daily light integrals (DLIs) for hydroponic lettuce (Lactuca sativa L.). In this study, effects of DLI and LED light quality (LQ) on growth, nutritional quality, and energy use efficiency of hydroponic lettuce were investigated in a plant factory with artificial lighting (PFAL). Hydroponic lettuce plants (cv. Ziwei) were grown for 20 days under 20 combinations of five levels of DLIs at 5.04, 7.56, 10.08, 12.60, and 15.12 mol·m−2·d−1 and four LQs: two kinds of white LEDs with red to blue ratio (R:B ratio) of 0.9 and 1.8, and two white LEDs plus red chips with R:B ratio of 2.7 and 3.6, respectively. Results showed that leaf and root weights and power consumption based on fresh and dry weights increased linearly with increasing DLI, and light and electrical energy use efficiency (LUE and EUE) decreased linearly as DLI increased. However, no statistically significant differences were found in leaf fresh and dry weights and nitrate and vitamin C contents between DLI at 12.60 and 15.12 mol·m−2·d−1. Also, no effects of LQ on leaf dry weight of hydroponic lettuce were observed at a DLI of 5.04 mol·m−2·d−1. White plus red LEDs with an R:B ratio of 2.7 resulted in higher leaf fresh weight than the two white LEDs. LUE increased by more than 20% when red light fraction increased from 24.2% to 48.6%. In summary, white plus red LEDs with an R:B ratio of 2.7 at DLI at 12.60 mol·m−2·d−1 were recommended for commercial hydroponic lettuce (cv. Ziwei) production in PFALs.
Genhua Niu, Minzi Wang, Denise Rodriguez, and Donglin Zhang
As high-quality water supply becomes limited in many regions of the world, alternative water sources are being used for irrigating urban landscapes. Therefore, salt-tolerant landscape plants are needed. Two greenhouse experiments were conducted to screen the salt tolerance of Zinnia marylandica (‘Zahara Coral Rose’, ‘Zahara Fire’, ‘Zahara Scarlet’, ‘Zahara Starlight’, ‘Zahara White’, and ‘Zahara Yellow’) and Z. maritima ‘Solcito’. In Expt. 1, plants were subirrigated with nutrient or saline solutions at electrical conductivity (EC) at 1.4 (base nutrient solution, control), 3.0, 4.2, 6.0, or 8.2 dS·m−1 for 4 weeks, whereas in Expt. 2, plants were surface-irrigated with the same nutrient or saline solutions for 4 weeks. In Expt. 1, all plants, regardless of cultivar, died by the end of the treatment at EC 6.0 and EC 8.2 as a result of high salinity in the root zone. Plants became shorter and more compact as EC of irrigation water increased. Shoot dry weight of all cultivars in EC 4.2 was reduced by 50% to 56% compared with that of the control. Shoot Na+ and Cl– accumulated excessively as salinity increased in the irrigation water, whereas Ca2+, Mg2+, and K+ did not change substantially. In Expt. 2, mortality varied with cultivar and treatment. Similar to Expt. 1, growth reduction resulting from elevated salinity across cultivars was found. Therefore, it is concluded that zinnia cultivars used in this study are sensitive to salinity and should not be planted in areas with high soil salinity or when alternative waters with high salinity may be used for irrigation.
Haijie Dou, Genhua Niu, Mengmeng Gu, and Joseph G. Masabni
Consumption of basil (Ocimum basilicum) has been increasing worldwide in recent years because of its unique aromatic flavor and relatively high concentration of phenolics. To achieve a stable and reliable supply of basil, more growers are turning to indoor controlled-environment production with artificial lighting due to its high environmental controllability and sustainability. However, electricity cost for lighting is a major limiting factor to the commercial application of indoor vertical farming, and little information is available on the minimum light requirement to produce uniform and high-quality sweet basil. To determine the optimal daily light integral (DLI) for sweet basil production in indoor vertical farming, this study investigated the effects of five DLIs, namely, 9.3, 11.5, 12.9, 16.5, and 17.8 mol·m−2·d−1 on basil growth and quality. ‘Improved Genovese Compact’ sweet basil was treated with five DLIs provided by white fluorescent lamps (FLs) for 21 d after germination, and gas exchange rate, growth, yield, and nutritional quality of basil plants were measured to evaluate the effects of the different DLIs on basil growth and quality. Results indicated that basil plants grown under higher DLIs of 12.9, 16.5, or 17.8 mol·m−2·d−1 had higher net photosynthesis, transpiration, and stomatal conductance (g S), compared with those under lower DLIs of 9.3 and 11.5 mol·m−2·d−1. High DLIs resulted in lower chlorophyll (Chl) a+b concentration per leaf fresh weight (FW), higher Chl a/b ratios, and larger and thicker leaves of basil plants. The shoot FW under DLIs of 12.9, 16.5, and 17.8 mol·m−2·d−1 was 54.2%, 78.6%, and 77.9%, respectively, higher than that at a DLI of 9.3 mol·m−2·d−1. In addition, higher DLIs led to higher soluble sugar percent and dry matter percent than lower DLIs. The amounts of total anthocyanin, phenolics, and flavonoids per plant of sweet basil were also positively correlated to DLIs, and antioxidant capacity at a DLI of 17.8 mol·m−2·d−1 was 73% higher than that at a DLI of 9.3 mol·m−2·d−1. Combining the results of growth, yield, and nutritional quality of sweet basil, we suggest a DLI of 12.9 mol·m−2·d−1 for sweet basil commercial production in indoor vertical farming to minimize the energy cost while maintaining a high yield and nutritional quality.
Min Lin, Terri W. Starman, Yin-Tung Wang, and Genhua Niu
The flowering time and flower quality of three hybrid Dendrobium nobile cultivars in relation to light intensity during cooling and duration of vernalization were studied in the first experiment. Mature Dendrobium Red Emperor ‘Prince’, Den. Sea Mary ‘Snow King’, and Den. Love Memory ‘Fizz’ plants were vernalized at 10 °C under 300 to 350 μmol·m−2·s−1 photosynthetic photon flux (PPF) (12-h photoperiod) or darkness, each with four cooling durations (2, 4, 6, or 8 weeks). Plants were forced in a greenhouse after vernalization. At least 4 weeks of 10 °C cooling in light was needed for complete flower initiation of Den. Red Emperor ‘Prince’, whereas Den. Sea Mary ‘Snow King’ and Den. Love Memory ‘Fizz’ only needed 2 weeks of 10 °C cooling regardless of light. For all three cultivars, darkness during vernalization slightly delayed flowering and resulted in fewer but larger flowers. Longer cooling duration delayed flowering, decreased flower longevity, and produced more and larger flowers. In a second experiment, Den. Love Memory ‘Fizz’ plants were vernalized at 15 °C for 4 weeks under a 12-h photoperiod and PPF of 0, 50, 100, or 200 μmol·m−2·s−1. Compared with 200 μmol·m−2·s−1, low PPF at 50 or 100 μmol·m−2·s−1 did not affect flowering time or flower qualities; however, darkness delayed flowering and reduced flower qualities except flower diameter.
Qiang Liu, Youping Sun, James Altland, and Genhua Niu
Tatarian dogwood (Cornus alba) is an ornamental shrub with white fruits, creamy-white flowers, and red stems in fall through late winter and is widely used in residential landscape, public parks, and botanical gardens. Two greenhouse experiments were conducted to characterize the survival, morphological, aesthetic, and physiological responses of tatarian dogwood seedlings to salinity and drought stresses. In Expt. 1, tatarian dogwood seedlings grown in three soilless growing substrates (Metro-Mix 360, 560, and 902) were irrigated with a nutrient solution at an electrical conductivity (EC) of 1.2 dS·m−1 (control) or saline solution (by adding calculated amount of sodium chloride and calcium chloride) at an EC of 5.0 or 10.0 dS·m−1 once per week for 8 weeks. Results showed that substrate did not influence the growth of tatarian dogwood seedling. All plants irrigated with saline solutions at an EC of 10.0 dS·m−1 died, whereas those irrigated with saline solutions at an EC of 5.0 dS·m−1 exhibited severe foliar salt damage with an average visual score of 1.0 (on a scale of 0 to 5, with 0 = dead and 5 = excellent without foliar salt damage). Compared with the control, saline solutions at an EC of 5.0 dS·m−1 reduced plant height and shoot dry weight (DW) by 50.8% and 55.2%, respectively. Relative chlorophyll content [soil plant analysis development (SPAD) reading], chlorophyll fluorescence (Fv/Fm), and net photosynthesis rate (Pn) also decreased when plants were irrigated with saline solutions at an EC of 5.0 and 10.0 dS·m−1. Leaf sodium (Na+) concentration of tatarian dogwood seedlings irrigated with saline solutions at an EC of 5.0 and 10.0 dS·m−1 increased 11 and 40 times, respectively, compared with the control, whereas chloride (Cl-) concentration increased 25 and 33 times, respectively. In Expt. 2, tatarian dogwood seedlings were irrigated at a substrate volumetric water contents (volume of water/volume of substrate, VWC) of 15%, 20%, 25%, 30%, 35%, 40%, or 45% using a sensor-based automated irrigation system for 60 days. Results showed that drought stress decreased plant growth of tatarian dogwood seedlings with a reduction of 71%, 85%, and 87% in plant height, leaf area, and shoot DW, respectively, when VWC decreased from 45% to 15%, but all plants survived at all VWC treatments. Significant reductions of photosynthesis (Pn), stomatal conductance (g S), transpiration rate (E), and water potential were also found in plants at a VWC of 15%, compared with other VWCs. However, SPAD readings and Fv/Fm of tatarian dogwood seedlings were similar among the VWCs. In conclusion, tatarian dogwood seedlings were sensitive to the salinity levels tested in this study but could survive at all tested substrate volumetric water contents and exhibited resistance to drought conditions.