Mexican elder (Sambucus mexicana Presl.) is used in arid landscapes of the Southwest, but the plant is known for its unpredictable performance in those landscapes. We studied drought responses of mexican elder plants grown in an arid environment using an in-ground nursery production system. Plants were maintained as well-irrigated controls or exposed to cyclic drought and irrigated based on evapotranspiration. Drought treatment lasted 165 days. Plants exposed to drought had more negative predawn and midday water potentials than well-watered plants. The ratio of variable to maximal fluorescence (Fv/Fm) for the drought group (0.76), was near the optimum value of 0.8, suggesting that chloroplasts of drought-stressed plants maintained high levels of activity. Drought cycle, but not drought treatment affected stomatal conductance. Drought-stressed plants had lower transpiration rates than controls except at drought cycle five when transpiration rates were similar between irrigation treatments. Relative water content was higher in controls (76%) than plants exposed to drought (66%). Leaf area of well-irrigated plants was over four times higher than that of plants exposed to drought. Leaf area to root dry weight ratio of drought-stressed plants was 79% lower than control plants. Severely reduced leaf area of drought-stressed plants might be one reason why landscape personnel conclude that mexican elder plants perform poorly in arid landscapes.
Cathleen Feser, Rolston St. Hilaire, and Dawn VanLeeuwen
Rolston St. Hilaire and William R. Graves
Differences in foliar morphology and anatomy of hard maples (Acer saccharum Marsh. and Acer nigrum Michx. f.) may explain contrasting responses to moisture stress of these species. We conducted a 2-year study to examine leaf morphology and anatomy of populations of hard maples indigenous near the 43°N latitude from 94°W longitude in Iowa to the 71°W longitude in Maine. Leaves were collected from shoots exposed to direct solar radiation on multiple trees at each of 24 sites in 1995, and at 36 sites in 1996. Samples collected in 1995 showed stomate frequency on the abaxial leaf surface ranged from 380 to 760 stomata/mm2. Mean guard cell pair width and length were 16 and 17 μm, respectively. Stomate frequency related quadratically to longitude, was greatest for leaves from Iowa, and was negatively correlated with mean annual precipitation of the sample site. Leaf thickness did not vary with longitude and averaged 96 μm. Palisade thickness showed a greater correlation than mesophyll thickness to total leaf thickness. Mesophyll thickness was more highly correlated than palisade thickness to specific leaf mass, which did not vary with longitude and averaged 5.2 mg·cm–2. Analysis of leaves collected over both years showed trichome frequency and lamina area were related quadratically to longitude; the largest and most pubescent laminae were from westerly sites. These studies are being coordinated with greenhouse experiments on responses of seedlings from selected populations to moisture deficits.
Rolston St. Hilaire and William R. Graves
Its more westerly native range and apparent xeromorphic foliar traits have led to speculation that black maple (Acer nigrum Michx.f.) is more drought resistant than sugar maple (Acer saccharum Marsh.). We examined differences in morphology and anatomy of leaves of trees of these species indigenous near the 43°N latitude in the midwestern and eastern United States. Leaves were collected during July, Aug., and Sept. 1995, from 10 trees in each of 24 sites at longitudes of 71°W in Maine to 94°W in Iowa. Density of trichomes on abaxial surfaces and lamina surface area showed quadratic relationships with longitude and were greatest for leaves from westerly sites in Iowa. The percentage of total lamina surface area partitioned in the two most basipetal lobes increased linearly with longitude. Abaxial surfaces had 6 to 960 trichomes/cm2, lamina surface area was 28 to 176 cm2, and surface area partitioned in basipetal lobes was 5% to 9%. A quadratic regression function related increases in trichome density to decreasing mean annual rainfall at collection sites. Specific leaf mass ranged from 3.5 to 7.6 mg·cm–2 and did not relate to longitude. Scanning electron microscopy showed leaves throughout the range had similar trichome morphology, and light microscopy is being used to examine variation in leaf anatomy and stomatal traits.
Rolston St. Hilaire and William R. Graves
Traits associated with drought resistance vary with provenance of hard maples (Acer sp.), but the stability of differences ex situ and over time is unknown. We compared growth, dry-matter partitioning, leaf anatomy, and water relations of seedlings from central Iowa, eastern Iowa, and the northeastern United States over 2 years. Some seedlings from each of the three provenances were used as well-irrigated controls. The remaining seedlings were drought-stressed and irrigated based on evapotranspiration. Across irrigation treatments, plants from Iowa had shorter stems and higher specific weight of lamina, root: shoot dry-weight ratios, and root: lamina dry-weight ratios than did plants from the northeastern United States when treatments began. Biomass partitioning did not differ based on provenance after irrigation treatment for 2 years, but leaves from central Iowa had a higher specific weight, and their abaxial surfaces had more stomates and trichomes, than did leaves from the Northeast. Drought stress reduced conductance only in plants from central Iowa. Across provenances, drought stress reduced stomatal frequency, surface area of laminae, and dry weights of laminae and roots, and increased root: shoot dry-weight ratio. Leaf water potential of plants subjected to drought was lower at predawn and higher at midday than that of control plants. Drought did not cause osmotic adjustment in leaves. We conclude that the stability of foliar differences among provenances of hard maples validates using these traits as criteria for selecting ecotypes for use in managed landscapes prone to drought.
Coye A. Balok and Rolston St. Hilaire
Identification of tree taxa that can thrive on reduced moisture regimes mandated by xeriscape programs of the southwest United States could be facilitated if responses to drought of those taxa are determined. Leaf water relations, plant development, and cuticular wax content of seven taxa maintained as well-irrigated controls or exposed to drought and irrigated based on evapotranspiration were studied. Leaf water potential of drought-stressed Fraxinus velutina Torr. (Arizona ash), Koelreuteria paniculata Laxm. (golden rain tree), Quercus macrocarpa Michx. (bur oak), and Quercus muehlenbergii Engelm. (chinkapin oak) were lower at predawn than the controls. Drought-stressed plants of F. velutina, K. paniculata, and Quercus lobata Née (California white oak) had more negative midday water potential than the control plants. Drought reduced stomatal conductance to as little as 17%, 23%, and 45% of controls in F. velutina, K. paniculata, and Q. macrocarpa, respectively. Drought-stressed plants of F. velutina, K. paniculata, Q. macrocarpa, and Q. muehlenbergii had reduced transpiration rates. Fraxinus velutina had both the highest net assimilation rate (NAR) and relative growth rate (RGR) regardless of irrigation treatment. Mean specific leaf weight (dry weight (DW) of a 1-cm2 leaf disc divided by the weight), trichome density, stomatal density, leaf thickness, and cuticular wax content varied among species but not between irrigation treatments. Leaves of Q. buckleyi Buckl. (Texas red oak) had one of the highest stomatal densities, and also had leaves which were among the waxiest, most dense, and thickest. Abaxial leaf surfaces of F. velutina were the most pubescent. Across species, drought led to lower ratios of leaf surface area to root DW, and leaf DW to root DW. Quercus buckleyi plants subjected to drought had the highest root to shoot DW ratio (3.1). The low relative growth rate of Q. buckleyi might limit widespread landscape use. However, Q. buckleyi may merit increased use in landscapes on a reduced moisture budget because of foliar traits, carbon allocation patterns, and the relative lack of impact of drought on plant tissue water relations.
Stefan Sutherin, Kevin Lombard, and Rolston St. Hilaire
To fill a critical need for more information on desert-adapted low water-use landscape plants, we created Southwest Plant Selector, a mobile plant selector application (app) of landscape plants from the New Mexico Office of the State Engineer (NMOSE) online database of New Mexico landscape plants. The app, which is available for Apple® iPhone® and iPad®, was designed for homeowners and lists plant descriptive items, pest and disease information, and a photograph of the plant. The app also displays a graphic of the three regions of New Mexico and El Paso County, TX, overlaid with text that provides plant water requirements for each of those regions. An associated web page allows users to calculate supplemental plant water requirements for individual plant selections. Homeowners can select landscape plants based on botanical or common name, plant type, and category, region, and sun exposure. Even with the specific user base of New Mexico residents who own Apple® mobile devices, the app has been downloaded nearly 5000 times since its first release on 29 June 2012. New Mexico State University (NMSU) Cooperative Extension Service agents now use the app as a part of their standard toolkit for interactions with homeowners. Mobile technologies such as smartphone and tablet apps are gaining prominence as outreach tools. Land-grant institutions can be more involved in leveraging mobile application technologies for education and outreach. This article describes a process that land-grant institutions can use to produce apps for horticulture outreach.
Rolston St. Hilaire, Cathleen F. Feser, Theodore W. Sammis, and Anderson S. St. Hilaire
Accurate measurement of evapotranspiration (ET) is difficult and expensive for large, in-ground container (pot-in-pot) plants. We engineered and used a simple and inexpensive system to determine evapotranspiration of in-ground container trees. The system was shop-assembled and used a block and tackle system attached to a collapsible tripod. A unique container harness system attached to the block and tackle system was used to lift containers that were sunken in the ground. Containers were weighed with a battery-operated balance that was accurate to 1 g (0.04 oz) at its maximum load capacity of 60 kg (132.3 lb). One person operated the system, and the weight of the system exclusive of the balance was 17.5 kg (38.50 lb). Gravimetric water use data obtained with the system werecombined with meteorological data to compute crop coefficients (Kc) of mexican elder (Sambucus mexicana). The system detected small changes in daily water use of mexican elder trees grown in 76-L (20-gal) in-ground containers. Crop coefficients ranged from 0.17 to 0.71. The acquisition of evapotranspiration data from relatively large, containerized landscape plants may be facilitated because the system is simple, inexpensive, and accurate.
Jane E. Spinti, Rolston St. Hilaire, and Dawn VanLeeuwen
We surveyed homeowners with residential landscapes in Las Cruces, N.M., to determine design features participants valued in their landscapes, their attitudes toward the landscape use of desert plants and opinions on factors that would encourage respondents to reduce landscape water use. We also determined whether the willingness to use desert plants in their landscapes related to the length of residency in the southwestern United States. At least 98% of respondents landscaped to enhance the appearance of their home and increase their property value. About half (50.6%) of the participants strongly agreed or agreed that the main reason to landscape was to display their landscape preferences. Many participants indicated they would use desert plants to landscape their front yard (80.3%) and back yard (56.3%), but relatively lower percentages of participants actually had desert landscapes in their front yard and back yard. Regardless of their property value, respondents were more likely to use desert plants in their backyard the shorter their stay in the desert. Data revealed that participants rank water shortages as the factor that would most likely cause them to reduce the amount of water they applied to their landscapes. We conclude that homeowners report willingness to use desert plants but desert-type landscapes are not a widespread feature of managed residential landscapes. Furthermore, water shortages and the length of time respondents spent in a desert environment would most likely influence water use in their landscapes.
Yahia Othman, Dawn VanLeeuwen, Richard Heerema, and Rolston St. Hilaire
Demand for New Mexico’s limited water resources coupled with periodic drought has increased the necessity for tree water status monitoring to guide irrigation scheduling of pecan (Carya illinoinensis) orchards. The objectives of this study were to assess the impact of water status developed during the flood irrigation dry-down cycles on photosynthesis (P n), and gas exchange [stomatal conductance (g S) to H2O (g H2O), transpiration (E), and intercellular CO2 (c i)] and to establish values of midday stem water potential (Ψsmd) that are needed to maintain P n and gas exchange of pecan. We conducted the study simultaneously on two southern New Mexico mature pecan orchards from 2011 through 2013. Flood irrigation as determined by grower practice was used on both orchards and P n, g H2O, E, and c i were assessed at Ψsmd of –0.4 to –2.0 MPa. Photosynthesis and gas exchange were higher in pecan trees shortly after irrigation than trees exhibiting water deficit near the end of a flood irrigation dry-down cycle. The decline in P n was markedly noticeable when Ψsmd dropped below –0.9 MPa. We attributed the reduction in P n mostly to stomatal limitation. The decline in P n and g H2O exceeded 50% when Ψsmd ranged from –1.5 to –2.0 MPa. For those reasons, we recommended that pecan orchards be maintained at Ψsmd higher than –0.90 MPa to prevent significant reductions in carbon assimilation and gas exchange.
Yahia Othman, Caiti Steele, Dawn VanLeeuwen, and Rolston St. Hilaire
For large fields, remote sensing might permit plant low moisture status to be detected early, and this may improve drought detection and monitoring. The objective of this study was to determine whether canopy and soil surface reflectance data derived from a handheld spectroradiometer can detect moisture status assessed using midday stem water potential (ψsmd) in pecan (Carya illinoinensis) during cyclic flood irrigations. We conducted the study simultaneously on two mature pecan orchards, one in a sandy loam (La Mancha) and the other in a clay loam (Leyendecker) soil. We were particularly interested in detecting moisture status in the −0.90 to −1.5 MPa ψsmd range because our previous studies indicated this was the critical range for irrigating pecan. Midday stem water potential, photosynthesis (A) and canopy and soil surface reflectance measurements were taken over the course of irrigation dry-down cycles at ψsmd levels of −0.40 to −0.85 MPa (well watered) and −0.9 to −1.5 MPa (water deficit). The decline in A averaged 34% in La Mancha and 25% in Leyendecker orchard when ψsmd ranged from −0.9 to −1.5 MPa. Average canopy surface reflectance of well-watered trees (ψsmd −0.4 to −0.85 MPa) was significantly higher than the same trees experiencing water deficits (ψsmd −0.9 to −1.5 MPa) within the 350- to 2500-nm bands range. Conversely, soil surface reflectance of well-watered trees was lower than water deficit trees over all bands. At both orchards, coefficient of determinations between ψsmd and all soil and canopy bands and surface reflectance indices were less than 0.62. But discriminant analysis models derived from combining soil and canopy reflectance data of well-watered and water-deficit trees had high classification accuracy (overall and cross-validation classification accuracy >80%). A discriminant model that included triangular vegetation index (TVI), photochemical reflectance index (PRI), and normalized soil moisture index (NSMI) had 85% overall accuracy and 82% cross-validation accuracy at La Mancha orchard. At Leyendecker, either a discriminant model weighted with two soil bands (690 and 2430 nm) or a discriminant model that used PRI and soil band 2430 nm had an overall classification and cross-validation accuracy of 99%. In summary, the results presented here suggest that canopy and soil hyperspectral data derived from a handheld spectroradiometer hold promise for discerning the ψsmd of pecan orchards subjected to flood irrigation.