pollen that can be transferred onto native lantana’s flowers by pollinators. Dehgan (2006) indicated the existence of a wide range of pollen stainability (from less than 5% in Patriot™ ‘Sunburst’ to more than 80% in ‘Professor Raoux’) in L . camara
David M. Czarnecki II, Amanda J. Hershberger, Carol D. Robacker, David G. Clark, and Zhanao Deng
R. H. Peterson and H. G. Taber
Most methods of staining pollen grains overestimate the extent of pollen fertility. The traditional staining method used for tomato pollen has been acetocarmine or cotton blue, which allows for a clear distinction between pollen grains with and without cytoplasm (2). However, pollen grains with cytoplasm are not necessarily fully fertile, as shown by germination on artificial media (2). A factor likely to be closely correlated with the viability of the vegetative cell of the male gametophyte is the state of the plasmalemma (1). On the basis of plasmalemma integrity, the important distinction between viable and nonviable pollen grains can be made with a vital stain such as fluoroscein diacetate.
M. L. Weaver, H. Timm, M. J. Silbernagel, and D. W. Burke
Viability of pollen grains of isogenic sibling bean (Phaseolus vulgaris L.) selections of known tolerance of sensitivity to high temperatures (HT), as previously determined by pod retention and seed yield, was compared to that of a common parent bean selection and a cowpea [Vigna unguiculata (L.) Walp.] cultivar. Exposure of newly opened flowers to temperatures of 35° or 41°C reduced the viability of pollen grains in all bean selections. Pollen of all sibling selections was less affected by HT than pollen of their common parent suggesting transgressive segregation of factors for HT tolerance. At 41°, most pollen grains were destroyed in the parent bean selection and the 2 HT-sensitive siblings, whereas 44% to 55% of the pollen grains appeared to be viable in the 2 HT-tolerant siblings. Pollen viability of the HT-tolerant cowpea cultivar was not reduced by temperatures to 41°. Pollen staining indicated an interrelationship between pollen viability and tolerance to HT stress among the bean selections. The technique described has the potential for rapid selection of HT-tolerant genotypes in hybrid populations.
Katsumi Suzuki, Tadashi Tsukaguchi, Hiroyuki Takeda, and Yoshinobu Egawa
Pod yield of `Kentucky Wonder' green bean (Phaseolus vulgaris L.) decreased at high temperatures due to a reduction of pod set. A highly positive correlation was observed between pod set and pollen stainability in flowers that were affected by heat stress about 10 days before anthesis. Pollen stainability was decreased by heat stress applied 8 to 11 days before flowering under controlled environment conditions. When mean air temperature during this period exceeded 28 °C, pollen stainability decreased under field conditions. Low pollen stainability indicated sensitivity to high temperatures about 10 days before flowering. A heat-tolerant cultivar showed higher pollen stainability than did heat-sensitive cultivars under high temperatures. These results demonstrated that heat tolerance at an early reproductive stage could be evaluated by analyzing pollen stainability using flowers developed under high temperatures.
Zhiyong Hu, Min Zhang, Qigen Wen, Jie Wei, Hualin Yi, Xiuxin Deng, and Xianghua Xu
flower buds collected from three trees before anthesis were sampled, all the pollens were freshly harvested. For pollen stain viability, one anther per flower bud was collected from six flower buds, mixed, and spread on 10 slides, and then three
Shih-wen Lin, Tsung-han Lin, Cynthia Kung Man Yee, Joyce Chen, Yen-wei Wang, Manoj Kumar Nalla, and Derek W. Barchenger
requires measuring fruit set over an extended time and is sensitive to variations in temperature that could permit an otherwise heat-sensitive plant to set fruit. Pollen viability is usually monitored by staining the harvested pollen and counting pollen
Keri D. Jones, Sandra M. Reed, and Timothy A. Rinehart
examined. Finally, fertility of triploid cultivars was estimated through pollen staining and controlled pollinations. Materials and Methods Plant materials. Cuttings were taken in Summer 2005 from a single plant each of 75 H. macrophylla
Renjuan Qian, S. Brooks Parrish, Sandra B. Wilson, Gary W. Knox, and Zhanao Deng
stainability on these porterweeds, which would be useful data in the breeding of noninvasive plants. Pollen staining has become a reliable method of determining pollen viability in hybridization studies ( Czarnecki et al., 2014 ). Other porterweed studies have
Clara E. Trueblood, Thomas G. Ranney, Nathan P. Lynch, Joseph C. Neal, and Richard T. Olsen
collected from flowers at anthesis for each plant and used for viability tests. Pollen grains were stained in 40 μL of 1% acetocarmine on a microscope slide. The slide was heated three times over a hot plate set at medium–low temperature to heat without
Hsiang-I Lee and Michael J. Havey
pollen stainability in onion families possessing S cytoplasm and segregating for the dominant allele at Ms from three different sources. We observed that male fertility restoration conditioned by one source of the dominant Ms allele was incomplete and