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Karen R. Harris, Brian M. Schwartz, Andrew H. Paterson, and Jeff A. Brady

and a tryptophan at the end of the kinase-2 domain indicates that all sequences have motifs of non-TIR-NBS-LRR resistance genes ( Meyers et al., 1999 ). Using the neighbor-joining algorithm, a dendrogram was created from all 44 BRGA, from which eight

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Karen R. Harris, W. Patrick Wechter, and Amnon Levi

nucleotide binding site–leucine-rich repeat (NBS-LRR) resistance genes. Sequences above the dashed line are Drosophila Toll and mammalian interleukin-1 receptor- nucleotide binding site (TIR-NBS), whereas sequences below the line are non-TIR-NBS

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David N. Kuhn, Giri Narasimhan, Kyoko Nakamura, J. Steven Brown, Raymond J. Schnell, and Alan W. Meerow

Identifying genetic markers linked to disease resistance in plants is an important goal in marker-assisted selection. Using a candidate-gene approach, we have previously developed genetic markers in cacao (Theobroma cacao L.) for two families of genes involved in disease resistance: non-TIR-NBS-LRR (Toll/Interleukin-1 Receptor-nucleotide binding site-leucine rich repeat) resistance gene homologues and WRKY transcription factor genes; however, we failed to isolate TIR-NBS-LRR genes. Using a novel algorithm to design degenerate primers, we have now isolated TIR-NBS-LRR loci as determined by DNA sequence comparison. These loci have been developed as genetic markers using capillary array electrophoresis (CAE) and single-strand conformational polymorphism (SSCP) analysis. We have mapped three distinct TIR-NBS-LRR loci in an F2 population of cacao and demonstrated that one is located on linkage group 3 and the other two on linkage group 5.

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Kelly J. Vining, Q Zhang, C.A. Smith, and T.M. Davis

of plant genomes. Whole genome sequences are available for: A. thaliana ecotype Col-0 and O. sativa ssp. japonica L. cv. Nipponbare. The A. thaliana genome of ≈157 Mb ( Bennett and Leitch, 2005 ) contains 149 NBS-LRR genes (55 non-TIR, 94 TIR

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Ke Cao, Lirong Wang, Gengrui Zhu, Weichao Fang, Chenwen Chen, and Pei Zhao

_111584) from the non-TIR-NBS-LRR class. The PCR reaction mixture and thermocycle protocols were the same as those used for SSR analysis. Amplified products of degenerate RGA primer pairs were separated on 1.2% agarose gels in 1 × TAE buffer. Desired bands

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Jingkang Hu, Yingmei Gao, Tingting Zhao, Jingfu Li, Meini Yao, and Xiangyang Xu

mechanisms Bioinformatics 28 2407 2411 Zhou, T. Wang, Y. Chen, J.Q. Araki, H. Jing, Z. Jiang, K. Shen, J. Tian, D. 2004 Genome-wide identification of NBS genes in japonica rice reveals significant expansion of divergent non-TIR NBS-LRR genes Mol. Genet

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virus infection were reduced at later inoculations. Linkage Mapping of NBS-LRR Disease Resistance Gene Analogs in Watermelon Karen Harris 1 , W. Patrick Wechter 2 , and Amnon Levi 2 ; 1 USDA-ARS, Crop Genetics and Breeding Research Unit, 115 Coastal