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Ningguang Dong, Qingmin Wang, Junpei Zhang, and Dong Pei

accurately reflect its active level. With the application of immunology in botany, it has become possible to detect in situ auxin in plant tissues. Immunohistochemical localization techniques have been used previously in maize [ Zea mays ( Shi et al., 1993

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Kurt D. Nolte, Gregory W. Erdos, and Karen E. Koch

Localization of sucrose synthasa (SS), an enzyme Previously shown to be highly active in transport tissues of citrus fruit, was further defined via immunohistochemical analysis of stage II calamondin fruit. Using the indirect immunogold technique, 8 μm sections were first reacted with rabbit anti-SS polyclonal serum followed by incubation with 5 nm gold conjugated goat-anti-rabbit IgG. Little immunolabel was observed in the majority of peel tissues, however an abundant immunoreaction was evident in parenchyma cells directly adjacent to the segment epidermis surrounding juice sacs. Antibody was not associated with this epidermnl layer. Similarly, in juice vesicle stalks (JVS) the internal parenchyma cells showed significant SS localization compared to minimal immunoreaction in the epidermal layers of the JVS. Although the antigen did not appear to be specifically localized within the vascular bundles, an extensive distribution of the enzyme was associated with the parenchymatous cells immediately adjacent to vascular strands.

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Shuguang Wang, Yongpeng Ma, Chengbin Wan, Chungyun Hse, Todd F. Shupe, Yujun Wang, and Changming Wang

reflect activity levels ( Dong et al., 2012 ). Therefore, immunohistochemical localization is an important technique for evaluating endogenous hormones during the rapid growth of bamboos. In addition, information regarding the sites of IAA and ABA storage

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Ying Gao, Hao Liu, Ningguang Dong, and Dong Pei

) differentiation stages; SAM = shoot apical meristem; sq = squama; VB = vascular bundle; lp = leaf primordial; ( A–F ) bar = 100 μm, ( G ) bar = 200 μm. Indole-3-acetic acid immunohistochemical localization. Excised samples were immediately fixed in a 2% (w

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Ying Gao, Hao Liu, and Dong Pei

. Indeed, immunohistochemical localization studies have been performed in maize [ Zea mays ( Vysotskaya et al., 2007 )], arabidopsis [ Arabidopsis thaliana ( Aloni et al., 2003 )], the shoot apices of strawberry [ Fragaria ×ananassa ( Hou and Huang, 2005

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Huiling Wang, Wei Wang, Weidong Huang, and Haiying Xu

, P. Zhan, J.C. Huang, W.D. 2012 Chalcone isomerase gene from grape berry: CDNA cloning, tissue expression, and immunohistochemical localization Biol. Plant. 56 545 550 Wen, P.F. Chen, J.Y. Kong, W.F. Pan, Q.H. Wan, S.B. Huang, W.D. 2005 Salicylic acid

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Seth DeBolt, Renata Ristic, Patrick G. Iland, and Christopher M. Ford

24 Jan. 2001 with daytime maxima exceeding 40 °C on 5 d, which may have contributed to a localized change in TA levels. Fig. 2. Tartaric acid profiles throughout development of ‘Shiraz’ berries under three light regimes. Tartaric acid levels

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Tripti Vashisth and Anish Malladi

possibility of the presence of an AZ at this location in other Vaccinium species. Such a study may be complemented by the analysis of the localized expression of genes and immunohistochemical analysis using markers specifically associated with the AZs as has

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Franco Famiani and Robert P. Walker

C 3 photosynthesis, PEPCK is present in some species, but not others, and when present, it appears to be localized in certain tissues such as trichomes or vasculature ( Chen et al., 2004 ). PEPCK was abundant in the leaves of maize and P. maximum