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Samuel F. Hutton and John W. Scott

sweetpotato whitefly ( Bemisia tabaci ), the strains that cause Tomato yellow leaf curl virus (TYLCV) are the most widespread and well known. Begomovirus resistance in Fla. 7907C is conferred by the Ty-1 gene, which was advanced using marker

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Yuanfu Ji, John W. Scott, and David J. Schuster

genes from these wild species into cultivated tomato using traditional breeding approaches ( Scott et al., 1996 ). The first such gene, Ty-1 , was introgressed into tomato from S. chilense accession LA1969 ( Zamir et al., 1994 ). A second TYLCV

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Samuel F. Hutton, Jay W. Scott, and David J. Schuster

explained by the ty-5 genotype ( Fig. 1A ); other lines, however, demonstrated segregation that was not associated with SlNAC1 ( Fig. 1B–C ), indicating that an additional gene may be affecting resistance. In support of this, the mean disease severity of

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Jianjun Chen, Lijia Li, and Ying Wang

Diversity and evolution of Ty1- copia and Ty3- gypsy retroelements in the non-photosynthetic flowering plants Orobanche and Phelipanche (Orobanchaceae) Gene 387 75 86 Pearce, S.R. Harrison, G. Heslop-Harrison, P.J. Flavell, A.J. Kumar, A. 1997

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Martha Dávila, Dermot Coyne, Shree Singh, and Guenhwa Jung

The genes involved in F1 seedling abnormal development and lethality in inter-gene pool crosses have been designated as Dl1 (MesoAmerican=MA) and Dl2 (Andean=A) (Shii et al., 1980, J. Hered. 71:218–222). The different degrees of leaf crippling (C) in segregating populations of crosses was due to the interaction between the Dl1 or Dl2 loci, growing environment, and the lcr allele (Singh and Molina, 1996, J. Hered., In press). The objective was to identify RAPD markers linked to the genes for crippling (lcr) and seedling lethality (Dl) using the bulked segregation analysis procedure for F2 of MA × A crosses. Crosses were made between C lines, FB 10413-24-2, WA 7807-305, and TY 5578-220 and normal (N) parents and tester stocks for Dl1 and Dl2 genes. The F2 FB 10413-24-2 × Carioca segregated 13 N:3C. F3 families segregated 3N:1C. RAPD marker OPB-10 was linked to Lcr at 31.2 cM. F3 families segregated 1N:3C. RAPD marker OPO16 was linked to Dl1 at 27 cM. The F2 WA-7807-305 × Rio Tibagi segregated 3N:1C. RAPD marker OPS-03 was linked to Lcr at 32.6 cM.

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Yuanfu Ji, Jay W. Scott, David J. Schuster, and Douglas P. Maxwell

important tomato resistance genes ( Foolad and Sharma, 2005 ), including numerous TYLCV resistance genes ( Ji et al., 2007b ). The first such gene, Ty-1 , which originated from S. chilense accession LA1969, was mapped near a molecular marker (TG97) on

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Yuanfu Ji and John W. Scott

Poster Session 53—Vegetable Breeding 2 21 July 2005, 1:15–2:00 p.m. Poster Hall–Ballroom E/F

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John W. Scott, Samuel F. Hutton, and Joshua H. Freeman

curl virus (TYLCV) are among the most destructive, the most widespread, and the most well known. TYLCV-resistant cultivars are commercially available in many production regions, and most of these use the dominant Ty-1 gene. Generally, tomato growers

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Naomi Porret, Peter Cousins, and Christopher Owens

Grapevine (Vitis vinifera L.) is one of our oldest domesticated crops and economically the most important cultivated fruit crop in the world. Cultivated grapes show substantial diversity in fruit color, including: varying shades of black, red, pink, grey, white, and types with pigmented berry flesh. The majority of V. vinifera cultivars only possess anthocyanin pigmentation in the skin of the berry (also known as teinturiers). However, some cultivars possess berries with intensely pigmented flesh as well as skin, which is often also associated with greater pigmentation of vegetative tissues. The genetic control and inheritance of fruit color in grapevine is poorly understood, despite evidence that the primary determination of anthocyanin production appears to be controlled by a single dominant locus in V. vinifera with white fruit being a recessive character. Recently, it has been shown that the presence of Gret1, a Ty3-gypsy-type retro-transposon in the promoter region of a myb-like regulatory gene is present in white-fruited cultivars of V. vinifera and that allelic variation in this gene associates with several qualitative classes of grape fruit color. It has been observed that the red-flesh berry phenotype is similarly controlled by a single dominant locus. Considering the association of variation in VvmybA1 with grape berry skin color, it was hypothesized that DNA sequence variation in VvmybA1 would also be associated with genotypes showing intensely pigmented berry flesh. In this study, we show that allelic variation in VvmybA1 associates with the teinturier phenotype both in a panel of accessions possessing red-flesh as well as in a population of full-sibs segregating for the red-flesh phenotype.

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Samuel F. Hutton, John W. Scott, and Joshua H. Freeman

‘Fla. 8970’ is a fresh market hybrid tomato ( Solanum lycopersicum ) that has resistance to Tomato yellow leaf curl virus (TYLCV) conferred by the Ty-1 gene ( Verlaan et al., 2013 ) and to fusarium crown and root rot (FCR) incited by Fusarium