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Mikel R. Stevens, Shawn A. Chrisensen, Ammon B. Marshall, JoLynn J. Stevens, Peter Wenzl, Eric Hunter, Jason Carling, and Andrzej Killian

Recently, a technology known as DArT (diversity array technology) has been developed to increase throughput in marker assisted selection (MAS). DArT utilizes microarray technology as a method to potentially compare thousands of molecular markers in one test to a single DNA sample. We used DArT on two sets of interspecific tomato [Solanum lycopersicum (Fla 7613) × S. pennellii (LA 716 or LA 2963)] segregating populations (BC, F2, and F1). We compared over 300 segregating plants to 3840 random tomato genomic fragments. After the 3840 markers were prepared, it took about 2 weeks of laboratory time to perform the experiments. With experience, this time can be reduced. We identified a total of 654 polymorphic markers usable for developing a DArT tomato genetic map. Depending on the particular cross, 13 to 17 linkage groups were identified (LOD 3) per population. Most recently, the amplified polymorphic DNA (AFLP) technique has been used for rapid genetic mapping of large numbers of anonymous genomic fragments. Besides the additional effort and reagents using AFLPs compared to DArT, a desired AFLP polymorphic band is often difficult to clone and process into a PCR based marker, whereas in DArT all markers are already cloned and immediately available for such experiments. A drawback to DArT is that it requires specialized software and equipment and is technically demanding. However, once the equipment and software are secured, techniques are optimized, and segregating populations developed, marker throughput is increased by orders of magnitude. Although challenging, the application of DArT can dramatically increase MAS throughput, thus facilitating quantitative trait and saturated mapping research.

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Mobashwer Alam, Craig Hardner, Catherine Nock, Katie O’Connor, and Bruce Topp

originated from the same orchard, and progeny seedlings were probably sourced from the open-pollinated nuts of the same mother tree. Molecular studies using multiple marker systems (isozyme, AFLP, DArT, and microsatellite) showed that these two cultivars are

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Emily G. Tenczar and Vera A. Krischik

Dart's Gold’. The purplish leaves of ‘Monlo’ may be higher in anthocyanins, which can be feeding deterrents ( Harborne and Williams, 2000 ; Simmonds, 2003 ) to the specialist ninebark beetle ( Calligrapha spiraeae ) (Coleoptera: Chrysomelidae). The

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Rhiannon L. Wilson and William A. Hoch

greenhouse for analysis of pollen and seed germination and DNA content. Three cultivars that demonstrated sterility in our test plot, ‘Crispa’, ‘Dart's Red’, and ‘Neon Flash’, were further tested through hand-pollinations in the greenhouse. For these

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Thomas J. Brass, Gary J. Keever, D. Joseph Eakes, and Charles H. Gilliam

1 Graduate Research Assistant. 2 Professor. 3 Associate Professor. Supported in part by the Horticultural Research Institute, Washington, D.C.; Griffin Corp., Valdosta, Ga.; J. Frank Schmidt & Son Co., Boring, Ore.; and the Dart Container

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Rita L. Hummel, Marianne Elliott, Gary Chastagner, Robert E. Riley, Kathy Riley, and Annie DeBauw

to forests in coastal northern California and millions of dollars in losses in the nursery industry in California, Oregon, and Washington ( Dart and Chastagner, 2007b ; Hansen et al., 2005a , 2005b ; Werres et al., 2001 ). Many Phytophthora

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David C. Zlesak

-leafed forms (i.e., ‘Luteus’ and ‘Dart’s Gold’) and the compact green-leafed ‘Nanus’ have been landscape staples for decades. Several recent cultivars combine different foliage colors (gold and purple foliage on an individual plant) or foliage colors other than

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Keri D. Jones, Sandra M. Reed, and Timothy A. Rinehart

439 Levin, D.A. 2002 The role of chromosomal change in plant evolution Oxford University Press N.Y Mallet, C. 1994 Hydrangeas: Species and cultivars Vol. 2 Centre d'Art Floral Varengeville

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Sandra M. Reed and Timothy A. Rinehart

vase life for cut stems of Hydrangea paniculata Proc. Southern Nursery Res. Conf. 49 624 626 Mallet, C. 1994 Hydrangeas: Species and cultivars Vol. 2 Centre d'Art Floral Varengeville, France Mallet, C. Mallet, R. van Trier, H. 1992 Hydrangeas: Species

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Zhiyong Wang, Paul Raymer, and Zhenbang Chen

and DArT markers in a wide barley cross Theor. Appl. Genet. 115 383 391 Kimball, J.A. Zuleta, M.C. Martin, M. Kenworthy, K.E. Chandra, A. Milla-Lewis, S.R. 2012 Assessment of molecular variation within ‘Raleigh’ St. Augustinegrass using AFLP markers