intensive and time-consuming. The development of molecular markers provides the possibility of rapid screening of resistant germplasm for breeding. Moreover, molecular markers tightly linked to resistant genes also provide the basis for isolating and cloning
57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management
57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management
30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers
115 ORAL SESSION (Abstr. 227-233) Tree Fruits and Nuts: Genetics/heeding/Molecular Markers
this trait could be very helpful for introducing and selecting the jointless trait in tomato germplasm, especially for fresh-market backgrounds which are predominantly jointed. The objective of this project was to develop molecular markers linked to the
; Erdogan and Mehlenbacher, 1997 ; Yao and Mehlenbacher, 2000 ; Koksal, 2002 ). In recent years, researchers around the globe have investigated genetic relationships among hazelnut cultivars using several types of molecular markers, including randomly
Scientific, Waltham, MA) and quality was assessed by electrophoresis of DNA through 1% agarose gels. Molecular markers distinguishing onion cytoplasms were indel markers in an intergenic region ( Havey, 1995 ) and the accD gene ( von Kohn et al., 2013 ) in
., 2013 ). Although they are affected by environmental conditions, morphology and agronomic characteristics are helpful tools for the survival of plant species diversity. A wide range of molecular markers are used to estimate genetic polymorphism, and
Apomixis is asexual reproduction through seed. Apomixis in the genus Pennisetum is of the gametophytic (aposporous) type. Genes for apomixis have been transferred from a wild apomictic species (P. squamulatum) to pearl millet (P. glaucum) by conventional breeding to produce an obligately apomictic backcross 3 (BC3) plant (Dujardin and Hanna, 1989, J. Genet. Breed. 43:145). Molecular markers based on restriction fragment length polymorphisms and random amplified polymorphic DNAs were identified in BC3 that were shared only with the apomictic parent. Segregation of these informative markers in a BC4 population indicated that three linkage groups from P. squamulatum were present in BC3 and that minimal recombination between these alien chromosomes and those of the recurrent parent occurred. Transmission of only one of the linkage groups was required for transfer of apomixis. Recombination is essential for genetic mapping, thus we are beginning to map the informative molecular markers in an F, interspecific cross between pearl millet and P. squamulatum, a population that segregates for apomictic and sexual reproduction.