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1 Department of Botany. Maine Agricultural and Forest Experiment Station Publication 2404. The marker portion of this research was conducted in the lab of Stephen Kresovich in Griffin, Georgia. We thank Stephen Kresovich, Sharon Mitchell, Doug Butts

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Bulked segregant analysis was used to identify RAPD markers that display tight linkage to the Vf gene in apple (Malus sp.) that confers resistance to five races of apple scab [Venturia inaequalis (Cke.) Wint.]. We identified several new RAPD markers linked to Vf. The most tightly linked marker in the test population, S52500, was cloned and sequenced. A linkage map of the Vf region was developed using these markers, RAPD markers previously described by other laboratories, and the isozyme locus Pgm-1. An assay was developed for Vf by multiplexing the two markers closely flanking the Vf locus. This assay has a theoretical `escape' value (discarding a resistant plant) of 3% and an error rate (selection of a susceptible plant) of 0.02%.

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individuals are obtained in only one generation ( Morrison and Evans, 1988 ). Furthermore, the incorporation of other biotechnological tools to the breeding programs such as marker-assisted selection coupled with in vitro production of DH lines accelerates the

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(prickliness) and single nucleotide polypmorphism (SNP) molecular markers. For the prickliness characterization, young leaves (≈10 cm long) of hybrid plants were evaluated using the Leaf prickles descriptor of EGGNET [European Network for Eggplant Genetic

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30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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the 121 listed by Dirr (2009). Molecular markers may be useful in identification of cultivars where morphological identification falls short ( Laurentin 2009 ). Fingerprinting specific cultivars could also provide some protection to breeders seeking

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generated in a crossing program. In red raspberry, it can take 15 years from the time of the first cross to the release of a new cultivar ( Graham and Jennings, 2009 ). Advances in molecular genetics, in particular, the development of molecular markers, have

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57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management

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variation in chromosome number and genome size among species of the Betulaceae, some degree of microsatellite marker transferability is expected based on results in other plant families. Microsatellites, also known as simple sequence repeats (SSR), are

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30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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