Search Results

You are looking at 1 - 10 of 12 items for :

  • HortTechnology x
Clear All

performance through adjustment of the media nutrient content, and developing a method of ex vitro rooting. An additional objective was to evaluate retipping of recently micropropagated plants as a method of obtaining large quantities of clones for commercial

Open Access

Acclimatization and growth of in vitro-derived apple shoots of two apple scion apple cultivars were compared under fogged conditions in a greenhouse and in a commercial growth cabinet (Phototron). Plant survival rates of microcuttings of `Royal Gala' and `Jonagold' were significantly better when maintained in the Phototron units than when grown in a greenhouse under fog. The number and length of roots on microcuttings was significantly higher in the Phototron than under fog. In the present study, we demonstrated that the Phototron environment was better than a fogged greenhouse for establishing apple shoots ex vitro. However, the Phototron units are so small that they hold no more than 100 to 120 plants at a time. Therefore, the units will be of most value to growers or individuals in laboratories who do not have a constant need for acclimatization facilities. Growers who acclimatize many plants should continue to use fogging or misting facilities.

Full access

were transferred ex vitro to rockwool for rooting as described by Lubell-Brand et al. (2021 ). For ex vitro rooting, we used an RCBD with four replications, each consisting of eight microcuttings. The percent rooting of microcuttings was recorded at 4

Open Access
Authors: , , and

Hosta ×hybrid Tratt. `Blue Cadet' and Hosta tokudama Tratt. `Newberry Gold' were micropropagated in shaken liquid culture and on agar media, in conventional vessels and vessels modified for ventilation in vitro. Acclimatization under intermittent mist and growth in an outdoor nursery during the late spring and summer were monitored by dry weight analysis of sample plants every 4 days for a 60-day period (ex vitro growth). Results for `Newberry Gold' were 1) in vitro shoot growth was greater in liquid than agar culture, regardless of vessel; 2) shoots from agar or liquid culture grew at similar rates ex vitro; 3) ex vitro root growth was greater for liquid than agar cultured plants, regardless of vessel type. Results for `Blue Cadet' were 1) in vitro and ex vitro shoot growth was greater in liquid than agar culture regardless of vessel type and 2) ex vitro root growth was greatest for liquid cultured plants from conventional vessels. Ventilated vessels were generally beneficial for agar but not liquid culture. Benefits of liquid culture for micropropagation of Hosta found in vitro are at least maintained and sometimes enhanced during ex vitro growth in the mist bed and nursery.

Full access

) and Driver and Kuniyuki walnut medium (PhytoTech Laboratories, Lenexa, KS). (B) This jar produced 12 plantlets (with roots), and the tallest plant was 167 mm. (C) Three plants of each treatment were transferred to ex vitro conditions; 1 mm = 0

Open Access

(multiplication of propagules), Stage 3 (rooting of in vitro formed shoots), and Stage 4 (ex vitro acclimatization). Each stage is influenced by a number of variables, with medium composition during multiplication greatly affecting the rate of propagule production

Full access

distributions of CO2 concentration. Low air exchanges in the vessel correlated with decreased net photosynthetic rates, decreased total soluble sugars, and decreased starch, which may effect ex vitro performance. INDOOR CONTROLLED

Full access

accurate quantitative measurement. Separate studies are underway to evaluate the effects of root-promoting growth regulators on in vitro and ex vitro rooting of these species. Experimental design and data analysis. The experiment was set up as a randomized

Full access

Sumagic (uniconazole) promotes flower bud set on Camellia japonica J. Environ. Hort. 23 153 157 Cha-um, S. Puthea, O. Kirdamanee, C. 2009 An effective in-vitro acclimatization using uniconazole treatments and ex-vitro adaptation of Phalaenopsis orchid

Full access

shoot length per explant. Excised shoots from stage II were then placed on a rooting medium (stage III) supplemented with different concentrations of IBA and NAA for 60 d, or rooted ex vitro, depending on the species. To determine the best auxin

Open Access