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, trachelium, and zinnia stems. Control solutions. Cut stems were held in DI water (pH 3.1–4.2; EC 0 dS·m −1 ), DI water supplemented with 200 mg·L −1 8-HQC (pH 2.8–3.1; EC 0.12–0.15 dS·m −1 ), tap water (pH 6.3–7.1; EC 0.18–0.23 dS·m −1 ), or tap water with 8

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ruthenium red for 20 min and observed for red color ( Jensen, 1962 ). A 1% Alcian blue 8GX solution in 3% acetic acid (pH 2.5) was applied to tissue sections for 20 min, rinsed with water, and the sections were examined for blue color under visible radiation

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cultivars of blueberry and found that the maximum vegetative growth occurred at pH 4.0 and 4.5, but that there was no significant growth at pH 2.5 and 3.0. However, Schmid et al. (2009) used sulfur to acidify pine sawdust to a pH range of 3.8 and 4.2, and

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rootstock growth through increasing soil pH; 2) biochar can improve apple rootstock growth through increasing soil microbial biomass and hence increase nutrient mineralization and availability; and 3) biochar can increase apple rootstock growth in

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± 0.05 g of homogenized sample was added to a tared 15-mL centrifuge tube and the mass was recorded. Ten milliliters of 50% v/v aqueous ethanol acidified to pH 2 (≈1 mL 12.1 M HCL) was added to the 1-g sample and then mixed once every 5 min manually

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sd s were obtained from three biological replications. Petal vacuolar pH measurement. To measure the vacuolar pH, 2 g of fresh petals of the Pl and Pd flowers at the fully opened stage was ground in liquid nitrogen and centrifuged at 18,407 g n for

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Clear (Floralife Inc., Walterboro, SC) at double strength (2× = 32 mL·L −1 water; pH = 2.78; soluble solids = 1.4%). Crystal Clear contains no hormones and did not affect the rate of budbreak in comparison with water but did reduce bacterial growth in

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using 0.5 mL reagent three times. After discarding the upper ether phase, the lower aqueous phase was collected and adjusted to pH 2.8. The same process was performed four times for each sample. An appropriate amount of solution was filtered in the

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) and concentration of other nutrients, samples were acidified to pH2 using H 2 SO 4 and filtered through a 0.45-µm syringe filter (09-719 F; Thermo Fisher Scientific Inc., Waltham, MA). Samples for determination of soluble PO 4 -P only were not

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ethylenediaminetetraacetic acid, pH 2.0); 5-min reaction time] ( Mehlich, 1984 ). At the end of extractions, the supernatant was filtered before being analyzed by the ICP (USEPA method 200.7). The plant samples were dried at 65 °C for 72 h and ground in a Wiley Mill to pass

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