Microtubers (Solanum tuberosum cv Snowden) were produced in 1-L jar fermentors using a two-step method consisting of a shoot multiplication phase (21 days) followed by a tuberization phase (25 days). The plantlets were immersed in Murashige and Skoog (MS) liquid medium for 3.5 min every 4 h. Low concentrations of ancymidol (anti-gibberellic substance), particularly during the shoot multiplication phase, were essential for tuber initiation and development. A continuous supply of 2 μMol ancymidol during the two phases of culture decreased plant height, but produced >100 microtubers per jar. Although the tuber development phase was short (25 d), 25% of the microtubers produced were >0.5 g with 17.5% to 18.0% of dry-matter content.
Y. Desjardins and J. Abdulnour
Sunn-hemp, Crotalaria juncea L. cv. Tropic Sun was developed in Hawaii in 1982 and recently introduced to the island of Guam by USDA Soil Conservation Service as a potential green manure crop. An evaluation of various legumes at three different soil regimes revealed that sunn-hemp produced greater biomass than other plants. In the study of the effects of sunn-hemp in subsequent vegetable production, slightly greater canopy was observed for potato, Solanum tuberosum cv. Kennebec, with green manuring with sunn-hemp than without. Yield of head cabbage, Brassica oleracea var. capita cv. KK Cross, was higher with green manuring (1085.5g/head) than without (725.4g/plant). Competition between indigenous rhizobia and introduced inoculant seems to exist at some locations. Major constraints in using sunn-hemp as green manure on the island are its limited seed sources and requirements of additional labor. Education and promotion of using this legume in a long term soil-improving system is needed.
J.H. van den Berg, M.W. Bonierbale, E.E. Ewing, R.L. Plaisted, and S.D. Tanksley
Tuberization and stolonization of cuttings were used as a model system to assess response to photoperiod in segregating potato progenies. The progenies were from backcrosses of a diploid hybrid between Solanum tuberosum and the short day requiring S. berthaultii to both parent species. Restriction Fragment Length Polymorphism (RFLP) analyses had been performed on these progenies as a part of other investigations. The RFLP maps were used to identify the loci controlling the photoperiod responses characterized by the cuttings. In the S. berthaultii backcross population, one locus appeared to control the response of cuttings only under long photoperiods, and coincided with a locus detected for stolonization on whole plants; a second locus was effective for tuberization under short photoperiods but was not detected with certainty under long photoperiods. Data analysis for the second backcross population is currently underway.
Iron-deficiency symptoms are observed on some genotypes of potato (Solanum tuberosum L.) grown on high-pH and calcareous soils. seven potato cultivars differing in response to iron deficiency chlorosis (FeDC) were grown on high-pH (8.1), calcareous (38% calcium carbonate equivalent in surface 20 cm) and silty clay soil in the field (Beka'a Valley, Lebanon), to determine the effects of FeDC on tuber yield of cultivars sprayed with Fe. A significant interactions between cultivars and Fe spray treatment were noted for visual FeDC ratings and tuber yield. Even though only slight FeDC was noted on some cultivars receiving no Fe spray, tuber yields were significantly increased when sprayed with Fe. Some cultivars with moderate FeDC ratings did not show a significant increase in yield when sprayed with Fe while other cultivars did. Sprayed cultivars generally produced higher tube; yields than unsprayed ones. Indicating that Fe-deficiency chlorosis in the Mediterranean region may be a serious limitation to potato tuber yield.
W.B. Evans and D.D. Warncke
Single-plant microplots of `Russet Norkotah' potatoes (Solanum tuberosum L.) were grown outdoors in a 5 × 5 factorial RCBD of indigenous phosphorous level (200, 325, 450, 575, 700 kg·ha-1 Bray-Kurtz Pl extractable; McBride sandy loam) and banded triple super phosphate (0, 50, 100, 150, 200 kg P2O5/ha). Disease in the low P soil that was used to create the four lower P soil blends completely confounds response of the plants across indigenous P levels and might have accentuated responses within levels. Plants responded to fertilizer P with tuber yield increases of 100, 70, 40, and 10 percent within the 200, 325, 450, and 575 indigenous P levels, respectively. Fertilizer P also increased marketable yield and tuber P concentration. Neither indigenous nor fertilizer P altered tuber specific gravity.
Companion studies compare the responses of corn (Zea mays L.) and potato to indigenous soil P levels and quantify P uptake among potato cultivars in solution culture.
Robert W. Goth, Ernest W. Goins, and Kathleen G. Haynes
Potato leafroll virus (PLRV) is a serious aphid transmitted virus disease of potato (Solanum tuberosum L.). Field observations suggest that the cv. BelRus is tolerant to PLRV. Greenhouse grown BelRus and PLRV susceptible potato cvs. Green Mountain and Katahdin were tested for PLRV with enzyme linked immunosorbant assay (ELISA) and subsequently infested with PLRV infected green peach aphids (Myzus persicae). ELISA was used to test leaves from the top, middle and bottom portion of the plants at 7 day intervals beginning 7 days after aphid infestation. PLRV was detected in all tested locations of the Green Mountain and Katahdin plants 21 days after inoculation. In BelRus, throughout the 11 week test, PLRV was detected predominantly in the top portion of the plants and at low titres. These results suggest that tolerance to PLRV infection in the cv. BelRus may be due to suppression of virus replication.
George H. Clough
Potato (Solanum tuberosum) cv. `Russet Burbank' response to source of side-dress Ca fertilization applied at 0, 28 and 56 kg·ha-1 Ca on fine sandy loam soil was evaluated. Side-dress Ca source and rate did not affect number or total weight of tubers/hill, average tuber weight, or tuber macronutrient concentrations at mid-season. Tuber B concentration was significantly greater with the 12-0-0-10.5 source as compared to the check. Tuber Fe concentration decreased linearly as 22-0-0-7 rate increased from 0 to 56 kg·ha-1 Ca. No other micronutrient concentration was affected by the applied treatments. Calcium fertilization had no effect on tuber yield, grade distribution, or specific gravity. The predominant internal defect observed was brown center, which was reduced at harvest by side-dress Ca application. Internal quality and french fry color were evaluated after storage for 4 months.
Chen-Yi Hung, John R. Murray, Sarah M. Ohmann, and Cindy B.S. Tong
The color of red potato tubers is due to an accumulation of anthocyanins in periderm and peripheral cortex tissues. The objective of this study was to characterize changes in anthocyanin content and tuber surface color during tuber development. Using the red tuber-producing potato (Solanum tuberosum L.) cultivar Norland, we observed that chroma (intensity of redness) and anthocyanin content per unit of surface area of greenhouse-grown tubers decreased as tuber weight increased. There was no increase in hue (tint) during the same developmental periods. Using high-performance liquid chromatography (HPLC), we determined that pelargonidin and peonidin are the major anthocyanidins (aglycones of anthocyanins) in the tuber periderm. Northern blot analyses indicated that steady-state mRNA levels of dihydroflavonol reductase (DFR), an anthocyanin biosynthetic enzyme, continued throughout tuber development. These results suggest that anthocyanins are synthesized throughout tuber development, and that cell division and/or enlargement contribute to a decline in chroma and anthocyanin concentration.
Qin Chen and Hai Y. Li
An improved method is described for the isolation of potato metaphase chromosomes for karyotypic and cytogenetic studies. Root tips from diploid Mexican species, Solanum pinnatisectum (2n = 2x = 24) and tetraploid cultivated S. tuberosum (2n = 4x = 48) were given four different pretreatments. The synthetic pyrethroid, Ambush, was the most stable and effective pretreatment reagent, providing the highest percentage of mitotic chromosomes at metaphase and the best spread of countable chromosomes for cytogenetic studies. Compared with an Ambush pretreatment at concentrations of 100-400 ppm, 1 to 10 ppm Ambush produced more easily distinguished chromosomes, which can be useful for comprehensive observation and karyotype analysis in both 2x and 4x potato species. This improved technique for examining mitotic chromosomes will be helpful in describing karyotypes, characterization of new hybrids, and identifying chromosome structural changes that are important in breeding schemes.
Aref A. Abdul-Baki and Theophanes Solomos
The diffusion coefficient of CO2 in `Russet Burbank' potato (Solanum tuberosum L.) tubers was determined under steady-state conditions at 10 and 27C. The data showed that the skin is the main barrier to gas diffusion, with an average diffusion coefficient of 6.57 × 10-7 and 7.61 × 10-7 cm·s-1 at 10 and 27C, respectively. The flesh also presents an appreciable barrier to gas diffusion. The average diffusion coefficient of CO2 in the flesh was 2.00 × 10-4 and 2.24 × 10-4 cm·s-1 at 10 and 27C, respectively. Under regular storage conditions, the tuber is well aerated and the concentration of O2 at the center of the tuber is sufficient to maintain aerobic respiration.