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Yong In Kuk and Ji San Shin

leaf age. Native polyacrylamide gel electrophoresis and activity staining. The isozymes of APX were separated on nondenaturating polyacrylamide gels, according to methods described by Laemmli (1970) , with only slight modifications. Equal

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Choun-Sea Lin, Nien-Tzu Liu, De-Chih Liao, Jau-Song Yu, Chuang-Hwei Tsao, Chao-Hsiung Lin, Chih-Wen Sun, Wann-Neng Jane, Hsing Sheng Tsay, Jeremy Jian-Wei Chen, Erh-Min Lai, Na-Sheng Lin, Wei-Chin Chang, and Chung-Chih Lin

gel electrophoresis (SDS-PAGE) system (Mini-P III; Bio-Rad), and covered by 1% agarose containing bromphenol blue. The SDS-PAGE was performed at 100 V for 120 min, following which the gel was stained by Coomassie blue. The experiments were repeated

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Matthew A. Escobar, Andrew Shilling, Pine Higgins, Sandra L. Uratsu, and Abhaya M. Dandekar

tissue, but activity was absent within the shell in pellicle and kernel tissues. As a complement to spectrophotometric PPO activity assays, protein extracts were separated using native polyacrylamide gel electrophoresis and subjected to an in-gel PPO

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Metka Sisko, Branka Javornik, Aleksander Siftar, and Anton Ivancic

polyacrylamide gel electrophoresis – based analysis of commercially important North American cultivars HortScience 41 304 309 Gianfranceschi, L. Seglias, N. Tarchini, R. Komjanc, M. Gessler, C. 1998

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Xunzhong Zhang, Kehua Wang, and Erik H. Ervin

mixed with 100 μL of 2 × SDS buffer [0.125 mol·L −1 Tris/HCl, pH 6.8, 20% (v/v) glycerol, 0.01% (v/v) bromphenol blue, 200 m m dithiothreitol, and 4% (w/v) SDS] was used for Sodium dodeclysulfate polyacrylamide gel electrophoresis analysis ( Laemmli

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Rui Sun, Hui Li, Qiong Zhang, Dongmei Chen, Fengqiu Yang, Yongbo Zhao, Yi Wang, Yuepeng Han, Xinzhong Zhang, and Zhenhai Han

for 1 min; final extension at 72 °C for 5 min; and holding at 4 °C. The PCR products were separated using 8% polyacrylamide gel electrophoresis and then visualized by silver staining. The DNA ladder was “puc18/Msp I” (RealTimer BioTech Co., Beijing

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Huiling Wang, Wei Wang, Weidong Huang, and Haiying Xu

/v) polyvinylpyrrolidone. Protein concentration was determined as described in Bardford (1976) using bovine serum albumin as the standard. The separation of total proteins was performed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in 12

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Haiying Zhang, Jianguang Fan, Shaogui Guo, Yi Ren, Guoyi Gong, Jie Zhang, Yiqun Weng, Angela Davis, and Yong Xu

s, 55 °C for 20 s, and 72 °C for 90 s with a final extension at 72 °C for 8 min. The PCR products were analyzed using 6% polyacrylamide gel electrophoresis in 1× Tris borate ethylenediaminetetraacetic acid buffer. The gel was stained with silver

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Mason T. MacDonald, Rajasekaran R. Lada, A. Robin Robinson, and Jeff Hoyle

, B.D. 1990 One-dimensional polyacrylamide gel electrophoresis 1 91 Hames B.D. Richwood D. Gel electrophoresis of protein: A practical approach Oxford University Press New York, NY Howitt, C

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Hetal M. Kalariya, Guido Schnabel, Cesar Petri, and Ralph Scorza

μg) was used to perform sodium dodecyl sulfate–polyacrylamide gel electrophoresis using 15% Tris-HCl ready Gels (Bio-Rad Laboratories, Hercules, CA). Protein was transferred to an immunoblot polyvinylidene fluoride membrane (Bio-Rad Laboratories), and