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Wanmei Jin, Qiang Zhang, Sunzhong Liu, Qinping Wei, Wanmei Jin, Zongming Cheng, Xiaohui Xue and Tingzhen Yang

Cantini, C. Cimato, A. Autino, A. Redi, A. Cresti, M. 2008 Assessment of the tuscan olive germplasm by microsatellite markers reveals genetic identities and different discrimination capacity among and within cultivars J. Amer. Soc. Hort. Sci. 133 598 604

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Chandra S. Thammina, David L. Kidwell-Slak, Stefan Lura and Margaret R. Pooler

. Bot. 34 510 520 Gong, W. Duan, T. Zhang, D. 2012 Development of microsatellite markers from Cercis chinensis (Fabaceae) Amer. J. Bot. 99 e337 e339 Guichoux, E. Lagache, L. Wagner, S. Chaumeil, P. Léger, P. Lepais, O. Lepoittevin, C. Malausa, T

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Noémi Makovics-Zsohár, Magdolna Tóth, Dezső Surányi, Szilvia Kovács, Attila Hegedűs and Júlia Halász

. Merkouropoulos et al. (2017) also used a total of seven microsatellite markers that were enough to discriminate 54 plum cultivars. The genetic relationships among plum cultivars and genotypes of different origin were depicted using neighbor-joining cluster

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Wei Zhou, Hong Wang, De-Zhu Li, Jun-Bo Yang and Wei Zhou

developed 13 microsatellite markers from L. pinceana and tested their use in another Luculia species, L. yunnanensis Hu, which is also a distylous species. Materials and Methods Genomic DNA samples of L. pinceana were extracted from silica gel

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Jiang-Chong Wu, Jing Yang, Zhi-Jian Gu and Yan-Ping Zhang

, 2001 ) and paternity analysis ( Chaix et al., 2003 ). In this article, 20 polymorphic microsatellite markers isolated and characterized from M. oleifera are reported for which no SSRs have been developed to date. Materials and Methods Seeds were

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Pan-Hui Huang, Wen-Bin Yu, Jun-Bo Yang, Hong Wang and Lu Lu

al., 1996 ). In this study, we developed new microsatellite markers for further estimating the genetic diversity and population genetic structure of P. rex . Twenty-two individuals of P. rex collected from five geographical locations in China

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Raymond J. Schnell, Cecile L. Tondo, J. Steven Brown, David N. Kuhn, Tomás Ayala-Silva, James W. Borrone and Thomas L. Davenport

; Vrecenar-Gadus and Ellstrand, 1985 ). Borrone et al. (2008) used microsatellite markers on Florida avocados and demonstrated that when two complementary cultivars are planted together in almost equal numbers, most of the harvestable fruit result from

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Warren F. Lamboy and Christopher G. Alpha

The USDA-ARS Vitis genetic resources collections in Geneva, N.Y., and Davis, Calif., contain ≈3600 accessions of >35 species. Accurate and unambiguous identification of these grapes is essential for efficient and effective use of this germplasm. Previous workers have successfully used polymerase chain reaction (PCR)-generated SSRs to fingerprint cultivars of the wine and table grape species, V. vinifera. Building on this work, we conducted a test of five previously characterized SSR loci on 110 accessions of 25 grape taxa (21 Vitis species and 4 hybrids) to determine if they would satisfy our need for identifying cultivars within the USDA-ARS grape collections. Scorable SSR fragments were produced with all 550 primer-accession combinations, with no null loci observed. The loci were highly polymorphic, with 16 to 38 different alleles found at a locus. Heterozygosity values ranged from 0.464 to 0.818, while gene diversity values ranged from 0.875 to 0.955. Discrimination power at a locus varied from a low of 0.947 to a high of 0.987. Combined discrimination power of all loci was effectively 1.000, with 2 chances in 100,000,000 that two sexually, independently derived grape accessions would not be distinguishable using this set of five SSR loci. Two plants in the study that had previously been classified as belonging to different grape species were shown to have identical SSR fingerprints, showing that they almost certainly possessed the same genotype. Because SSR markers are codominant and highly polymorphic and SSR loci are generally conserved across a range of related species, we strongly recommend SSRs for fingerprinting not only grape, but other clonal genetic resources collections as well.

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Gayle M. Volk, Christopher M. Richards, Adam D. Henk, Ann Reilley, Diane D. Miller and Philip L. Forsline

individuals representing 88 half-sib families collected in the forests of Kazakhstan were compared using seven unlinked microsatellite markers ( Richards et al., 2009 ). Classification of individuals into four clusters based on Bayesian assignment tests

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Kirk W. Pomper, Jeremiah D. Lowe, Li Lu, Sheri B. Crabtree, Shandeep Dutta, Kyle Schneider and James Tidwell

), peach at 2.2 ( Ahmad et al., 2004 ), and avocado in Ghana at 4.4 ( Acheampong et al., 2008 ). Microsatellite marker loci have been used to study two species of Annona L., which are relatives of pawpaw. In Annona crassiflora Mart., an undomesticated