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Zhou Li, Yan Peng, and Bingru Huang

, 2004 ; Pessarakli, 2007 ). Drought and heat stress injury are typically characterized by leaf dehydration, reflected as a decline in leaf water content or accelerated leaf senescence due to loss of chlorophyll and photosynthetic activities, as well as

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Keun H. Cho, Veronica Y. Laux, Nathan Wallace-Springer, David G. Clark, Kevin M. Folta, and Thomas A. Colquhoun

measurement. The plants were dried inside an oven at 75 °C for 48 h and weighed. Leaf chlorophyll pigment was extracted from 100 mg of fresh leaves at 65 °C for 30 min in a 10-mL dimethyl sulfoxide solution (Thermo Fisher Scientific Inc., Hampton, NH). Each

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Xingbin Xie, Congbing Fang, and Yan Wang

flavor and texture, fruit characteristic skin color is important for consumer acceptance and often an indicator for ripening of european pears ( Charoenchongsuk et al., 2015 ; Kappel et al., 1995 ). Yellowing caused by chlorophyll degradation in storage

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Robert E. Rouse

The Minolta chlorophyll meter SPAD-502 (Minolta Camera Company, 101 Williams Drive, NJ 07446, USA) has been found to be a quick, accurate, simple, and nondestructive way to determine chlorophyll content in citrus leaves and a standard curve had been developed. The SPAD-502 chlorophyll meter was used to measure chlorophyll content in citrus leaves of ten varieties on three rootstocks. Leaf mineral analysis was then determined on these leaves for N, P, K, Mg, Mn, Zn, Fe, Cu and Ca. Correlation r values were generally low and not significant for most nutrient elements but were highest for Fe and Ca. The relationship of leaf nutrient levels and chlorophyll meter readings are not understood. The usefulness of the SPAD chlorophyll meter for determining mineral content in citrus leaves is not yet known.

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Valentina Schmitzer, Maja Mikulic-Petkovsek, Franci Stampar, and Vlasta Cunja

, carotenoids, chlorophylls, and flavonols are the major contributors to astonishing color diversity of flowers, and their content often changes during flower senescence ( Schmitzer et al., 2010 ; Slatnar et al., 2013 ; Sood and Nagar, 2003 ). Moreover, petal

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E.J. Ryder, Z.H. Kim, and W. Waycott

Three mutant traits for chlorophyll deficiency in lettuce (Lactuca sativa L.), bleached bud, calico-2, and pale green, are inherited as single recessive alleles. Bleached bud is epistatic to another recessive allele, dappled. Calico-2 is epistatic to dappled. Pale green is hypostatic to chlorophyll deficient-3. The Vanguard cd mutant is the same as chlorophyll deficient-3. The light green mutant 8744-1 is the same as light green. Independent inheritance is shown for bleached bud and dappled, calico-2 and dappled, and pale green and chlorophyll deficient-3, respectively.

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David A. Felicetti and Larry E. Schrader

chlorophyll and carotenoid analysis, and two disks were used for phenolic analysis. Thus, each degree (class) of sunburn had 10 repetitions, with each repetition composed of two peel disks from the same apple. In 2006, 20 apples of sunburn degrees 0 to 4 were

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Fan Zhang, Zi Wei, Peter Jeranyama, Carolyn DeMoranville, and Harvey J.M. Hou

, including spectrometry, high-performance liquid chromatography (HPLC), and chlorophyll fluorescence kinetics to examine cranberry leaves with yellow vine syndrome and normal cranberry leaves to provide novel insight into the underlying mechanism causing the

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Kui Lin, Zhi Huang, and Yong Xu

techniques of plant physiological biochemical experiment, p. 190–192. Higher Educ. Press, Beijing, China (in Chinese) Wang, H. Gu, M. Cui, J.X. Shi, K. Zhou, Y.H. Yu, J.Q. 2009 Effects of light quality on CO 2 assimilation, chlorophyll-fluorescence quenching

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W.C. Lin, J.W. Hall, and A. Klieber

A video-imaging technique, using commercial software to process images obtained at 550 nm, was established to estimate chlorophyll content of cucumber fruit disks. The chlorophyll content of excised disks was extracted, determined, and regressed on the video-image grey level. They were linearly related. The change in grey level of the whole visible image accurately indicated the change of green color during fruit development on the vine and the loss of green color after 1 week of storage at 13C. The relationship of the chlorophyll content on grey level was quadratic for three imaging methods: 1) average grey level of the five disks; 2) average grey level of the whole cucumber image; and 3) average grey level of central one-third of the whole cucumber image. Chlorophyll content was most highly correlated to the grey level of the disks themselves (residual SD = 6.74 μg·cm-2), but this sampling technique was destructive. Both one-third of the fruit image (SD = 9.25 μg·cm-2) and the whole image (SD = 9.36 μg·cm-2) provided satisfactory precision. For simplicity, whole-fruit imaging is suitable for estimating fruit chlorophyll content and for quantifying fruit green color intensity. Potential use of this technique in product sorting and shelf life prediction of long English cucumbers is discussed.