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pH, 2) to assess the effect of medium pH range on apple subculture proliferation, rooting, and adventitious bud regeneration from leaves, and 3) to determine the necessity of pH adjustment while preparing medium for apple tissue culture. Materials and

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measured using a Metrohm 800 Dosino 862 Compact Titrosampler (Metrohm AG, Herisau, Switzerland) and an electrode standardized to pH 2.00, 4.00, 7.00, and 10.00 buffers. Titratable acidity was determined from 6 g of juice diluted with 50 mL deionized

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. Fig. 1. Relationship between substrate pH and chlorophyll meter readings (SPAD-502; Minolta, Ramsey, NJ) or shoot dry weight (SDW) of ‘Pacifica Blush’ annual vinca grown in switchgrass substrates; SPAD = −2.29 × pH 2 + 29.10 × pH − 38.09, R 2 = 0

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pathway ( Forkmann, 1991 ; Holton and Cornish, 1995 ; Mol et al., 1998 ; Wiering and deVlaming, 1984 ). Additionally, genetic differences in flower color resulting from modifications in the pH were explained by genes Ph1, Ph2 , and Ph6 ( Griesbach

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their F 1 progeny were resolved in 5 mL of a mobile phase consisting of acetonitrile–0.3% phosphoric acid adjusted to pH 2.2 with triethylamine (1:7). Analytic samples were filtered through a Whatman 13-mm × 2-μm nylon syringe filter into 2-mL screw cap

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growth stage, relative leaf elongation rate (RLER) was calculated as followed: where ln PH 2 and ln PH 2 are the natural logarithms of the PH at time one t 1 and time two t 2 , respectively. Plants were harvested from 4.5 m plots when an average of

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acidity, combined acidity, and the pH of brine samples were periodically measured in a titroprocessor (Metrohm 670; Herisau, Switzerland). Analyses of free and combined acidity were made by titrating up to pH 8.3 with 0.2 N OHNa, and down to pH 2.6 with 2

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-18e resin). Elution was performed in a stepwise gradient with a water (pH 2.6 adjusted with H 3 PO 4 )/acetonitrile (ACN) volumetric ratio of 0 min, 7% ACN; 0 to 20 min, 20% ACN; 20 to 28 min, 23% ACN; 28 to 40 min, 27%, ACN; 40 to 45 min, 29%, ACN

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chelate-associated Fe solubility is 1) a function of substrate pH; 2) chelating agent stability constants with other cations like Ca, Cu, Mn, and Zn; and 3) relative abundance of these cations ( Boxma, 1981 ; de Kreij, 1998 ; Lindsay and Norvell, 1969

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of ethanol 70% and 30 mL of HCl 0.1%, pH 2.0) for 2 min in a blender, then placed in a beaker covered with parafilm and aluminum foil, and extracted in darkness for 12 h at 4 °C. Afterward, a filtration was made, transferring the content to a 250-mL

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