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Soil, Deli, Fuzhou, China; 1:1, v/v), and established in the greenhouse for simultaneous ex vitro rooting. The rooting percentage was calculated as follows: Table 4. Effect of 1-naphthaleneacetic acid on root induction in micropropagated plantlets of H

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preparation, use of feeder layers, complex media formulations, and numerous subcultures. The aim of the present study was to establish a simple and efficient protocol to obtain transgenic tomato plants of cv. Micro-Tom, including an optimized ex vitro

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production. The losses during acclimatization accounted for 20% to 50% of the plantlets ex vitro. Photoautotrophic micropropagation (PA), using a sugar-free medium and leafy explant, in which plantlets use CO 2 in the air as the sole carbon source, has

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.75 ± 0.03, solidified with 7.5 g·L −1 agar, and maintained under standard culture conditions for 1 week. Rooted microshoots were then transferred ex vitro into 72-cell trays and maintained under mist in a completely randomized design. After 4 weeks

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, this article describes a complete micropropagation system involving disinfection, in vitro multiplication, rooting, and hardening followed by ex vitro acclimatization procedures used to attain that objective, thus producing thousands of Aloe vera

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rooting percentage in a previous study ( Martini and Papafotiou, 2009 ). For acclimatization, plantlets from adult plants or sprouts of burned plants with well-developed roots were transferred ex vitro to 500-mL plastic containers on a mixture of peat

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Photosynthesis and growth of in vitro-cultured strawberry plantlets (Fragaria × ananassa Duch. cv Kent) were investigated during a 4-week in vitro culture in a rooting medium and a 4-week ex vitro period. The leaves formed in vitro on a medium containing sucrose developed a positive photosynthetic capacity. At transplanting to the ex vitro environment, their photosynthetic rate was 12.76 μmol CO2/m2 per second, which was as high as that of leaves generated and grown in the greenhouse. During the ex vitro period, photosynthetic rates of in vitro-generated leaves decreased and dark respiration rates increased. However, in vitro leaves were photosynthetically active throughout the 4 weeks ex vitro. In the first 2 weeks of the ex vitro period, in vitro-generated leaves had an important contribution to the overall plantlets' photosynthetic capacity.

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 al. 2018 ). Critical steps in blackberry micropropagation are the acclimatization of plants on ex vitro transplantation and plant growth ( Dewir et al. 2022 ). The mycorrhization of in vitro-propagated plants using arbuscular mycorrhizal fungi (AMF) is

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IV, factors that affect ex vitro establishment and acclimation were examined. The information presented serves as a foundation for the successful micropropagation of hemp. Materials and Methods Plant material The hemp cultivar TJ’s CBD was

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and multiplied in vitro were rooted ex vitro. Microshoots were dipped in indole-3-butyric acid rooting hormone (Hormidin 1; OHP Inc., Mainland, PA) and rooted in clear plastic salad trays containing a mix of 1 peat: 1 vermiculite. A completely

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