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-pure water. Sample and standard preparation. To extract organic acids, we precision-weighed 5.0 g of V. uliginosum fruit from each sample and added 5 mL of mobile phase (0.01 mol·L −1 K 2 HPO 4 , pH 2.5) before grinding the sample into a homogenate. We

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)] in tap water (pH 2.8, EC 0.49 dS·m −1 ); 3) 10 g·L −1 a propriety mixture of sugar, acidifier and a biocide [FLO (Floralife ® Crystal Clear packets; Floralife, Walterboro, SC)] in tap water (pH 3.1, EC 0.50 dS·m −1 ); 4) deionized water (pH 3.8, EC

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models, Eqs. [3] through [7] , were obtained by fixing three factors as the zero levels, whereas the other three factors were set as an explanatory variable (as shown in Figs. 2 and 3 ): y PH = 2 . 696 + 0.295 w + 0 . 216 w 2 [3] y CD = 8 . 414 + 0

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chromatography (HPLC) separation of tartaric, malic, and oxalic acids was achieved using a Prevail Organic Acid column 250 × 4.6 mm, 5-μm (Alltech Associates, Deerfield, IL); the mobile phase was 25 m m KH 2 PO 4 adjusted to pH 2 with phosphoric acid at a flow

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.L. Methods of soil analysis. Part 2 2nd Ed Agron. Monogr. 9. ASA and SSSA Madison, WI Landschoot, P. 2007 Managing soil pH in turf Grounds Maintenance, Penton Media, Inc 21 Nov. 2007 < http://grounds-mag.com/mag/grounds_maintenance_managing_soil_ph

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Waters 600E pump (Waters Corp., Milford, MA). Phenolic acids were eluted using a mobile phase consisting of 1% (by volume) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. The column temperature was set to 30 °C and an

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interlinked columns Supelco LC-18 (250 × 4 mm); 30 °C; mobile phase: 1% phosphoric buffer (pH = 2.5), flow rate: 0.8 mL·min −1 . Anthocyanins were determined by pH differential method ( Wrolstadt, 1976 ) with Cary 300E spectrophotometer (Varian, Australia

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were eluted using a mobile phase consisting 1% (v/v) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. Isocratic elution was performed with a flow rate of 0.75 mL·min −1 . Peaks were detected at 320 nm using a Waters 2487 dual

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. Grimsley, N. 1998 Genetic mapping of Ph-2 , a single locus controlling partial resistant to Phytophthora infestans in tomato Mol. Plant Microbe Interact. 11 259 269 10.1094/MPMI.1998.11.4.259 Morrison, L. Skaggs

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each harvest date. Soluble solids content was calculated using a refractometer. Titratable acidity and pH were measured by Metrohm 800 Dosino 862 compact titrosampler and electrode standardized to pH 2.00, 4.00, 7.00, and 10.00 buffers (Metrohm AG

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