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Mahonia have been successfully achieved both in vitro and ex vitro using a variety of auxins. Mackay et al. (1996) achieved nearly 100% rooting of M. trifoliata in vitro using 1.0 μM naphthaleneacetic acid on cultures younger than 6 months. However

abnormal. Statistical analysis. Ex vitro results were analyzed using MINITAB® release 14 statistical package (Minitab Inc., State College, PA). One-way analysis of variance was conducted to test Fisher's significance level at 5%. Percentage germination data

inconsistent. Furthermore, attempts to promote vegetative and root growth during ex vitro acclimatization have achieved limited success. Thillerot et al. (2006) reported very slow growth of P. cynaroides explants with no root growth after 2 months in ex

relative to some woody plants with episodic growth, such as species of Fagus and Juglans ( McCown and McCown, 1987 ). The initial ex vitro rooting treatment using a 10% auxin solution yielded somewhat low mean (± se ) rooting percentages of 30 ± 5

shoot formation. ( A ) Flowering plant. ( B ) Multiple shoot formation from protocorms in Murashige and Skoog medium + 13.3 μM N 6 -benzyladenine. ( C ) In vitro-rooted shoots ready for ex vitro culture. ( D ) Plants obtained from in vitro culture after

Paphiopedilum micropropagation from ex vitro–derived explants has been relatively limited. Its difficulty has been caused by contamination of ex vitro–derived explants and the poor development of explants ( Huang, 1988 ; Stewart and Button, 1975 ). There have

Soil, Deli, Fuzhou, China; 1:1, v/v), and established in the greenhouse for simultaneous ex vitro rooting. The rooting percentage was calculated as follows: Table 4. Effect of 1-naphthaleneacetic acid on root induction in micropropagated plantlets of H

BA 1 mg·L −1 and NAA 0.1 mg·L −1 ; ( E ) in vitro rooted shoots ready for ex vitro culture; ( F ) plantlets of O. tigrinum after successful establishment in soil for 2 months. Bar = 1 cm. PLBs = protocorm-like bodies; NAA = α-naphthaleneacetic acid

preparation, use of feeder layers, complex media formulations, and numerous subcultures. The aim of the present study was to establish a simple and efficient protocol to obtain transgenic tomato plants of cv. Micro-Tom, including an optimized ex vitro

production. The losses during acclimatization accounted for 20% to 50% of the plantlets ex vitro. Photoautotrophic micropropagation (PA), using a sugar-free medium and leafy explant, in which plantlets use CO 2 in the air as the sole carbon source, has