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Aaron E. Walworth and Ryan M. Warner

dehydration resulting from extracellular ice formation is a critical component of cold acclimation. Electrolyte leakage assays provide a simple method to assess cellular membrane integrity of plants exposed to abiotic stresses, including low temperature

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Chae Shin Lim, Seong Mo Kang, Jeoung Lai Cho, Kenneth C. Gross and Allan B. Woolf

ethylene production, respiration, and electrolyte leakage (EL) usually precede or occur concomitantly with appearance of visible symptoms ( Purvis, 2002 ). Peppers have been classified as nonclimacteric fruit ( Lurie et al., 1986 ). However, the pattern

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M.E. El-Mahrouk, A.R. El-Shereif, Y.H. Dewir, Y.M. Hafez, Kh. A. Abdelaal, S. El-Hendawy, H. Migdadi and R.S. Al-Obeed

water and placed in 50% glycerol before evaluation. Discoloration of stem discs resulting from NBT or DAB staining was quantified using a ChemiImager 4000 digital imaging system (Alpha Innotech Corp., San Leandro, CA). Electrolyte leakage. Measurements

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Jinyu Wang, Patrick Burgess, Stacy A. Bonos, William A. Meyer and Bingru Huang

, at the end of drought stress, whereas EL of hard fescues, sheep fescues, and slender creeping red fescues reached 19% to 32%, 21% to 23%, and 29% to 33%, respectively, at the end of drought stress. Fig. 3. Electrolyte leakage of ( A ) chewings fescue

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Eunkyung Lee, Steven A. Sargent and Donald J. Huber

trilocular, therefore two or three measurements, respectively, were made per fruit, and five individual fruits were evaluated per treatment every other day. Electrolyte leakage. To examine the effect of impact on tissue disruption, electrolyte leakage

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Aditi Satpute, Bryce Meyering and Ute Albrecht

either 3.5 °C or 7 °C. CI symptoms were rated at day 3, 6, and 9 of storage; leaf electrolyte leakage (LEL), total weight loss (TWL), and phenolic content were assessed on day 9 of postharvest storage (see respective sections below). A separate set of

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Farzad Nazari and Mahmoud Koushesh Saba

, 3, 5, 7, 9, and 12, and also Wt 0 was the weight of the same stem (g) at t = day 0 ( He et al., 2006 ). Electrolyte leakage (EL). EL of petal or stem-end was determined (at 0, 5, and 12 vase periods) using Sairam et al. (1997) method at which 0

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Xunzhong Zhang, Kehua Wang and Erik H. Ervin

23 kDa dehydrin polypeptide was associated with freezing tolerance of zoysiagrass ( Zoysia Willd.). The electrolyte leakage technique is commonly used to assess the level of cell injury caused by low temperatures and to test the relative freezing

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Ockert Greyvenstein, Terri Starman, Brent Pemberton, Genhua Niu and David Byrne

-induced loss of the semipermeability of the plasma membrane, the tonoplast, or other membranes within the cell ( Berry and Björkman, 1980 ). Cell MTS makes use of a conductivity test to measure the amount of electrolyte leakage from leaf disks. Cell membrane

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Qi Chai, Fang Jin, Emily Merewitz and Bingru Huang

membrane stability [electrolyte leakage (EL)], leaf chlorophyll content (Chl), and leaf photochemical efficiency (Fv/Fm) were measured at 0, 5, 10, 15, and 20 d of drought stress and 3 and 7 d of re-watering. During 3 and 7 d of re-watering, these