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cotyledon explants showed the same time course of morphological and histological changes as previously described ( Ermel et al., 2000 ; Gutmann et al., 1996 ; Jay-Allemand et al., 1991 ). The distribution of IAA in the walnut cotyledon was revealed by

with six flowers; additional flowers were prepared for scanning electron microscopy (SEM). The classification and staging of flowers were based on very specific macroscopic flower characteristics. Thus, within a stage, histological development was found

.J. Morgan, E.R. 2012 Temperature impacts changes in crown buds and flowering of gentian ‘Spotlight’ Sci. Hort. 143 49 55 Saravitz, C.H. Blazich, F.A. Amerson, H.V. 1993 Histology of in vitro adventitious bud development on cotyledons and hypocotyls of fraser

, J.A. Blanchette, R.A. Zambino, P.J. David, A. 2003 Histology of white pine blister rust in needles of resistant and susceptible eastern white pine Plant Dis. 87 1026 1030 Knight, R

18 389 394 Monosmith, H.R. 1928 An investigation of the histological development and inheritance of male sterility in a clone of Allium cepa L PhD Diss., Univ. California Berkeley Ockendon, D

.B. Kraus, J.E. Romano, E.C. 2000 Hormonal and histological studies related to in vitro banana bud formation Plant Cell. Tiss. Org. Cult. 63 187 192

strawberry, Fragaria × ananassa , and related wild germplasm Euphytica 136 139 150 Meric, C. Dane, F. Olgun, G. 2003 Histological aspects of anther wall in male fertile and cytoplasmic male sterile Helianthus annuus L. (sunflower) Asian J. Plant Sci. 3 145

, 1990 ). Some histological studies of organ development have been reported for a range of species ( Stevenson, 1988 . and citations therein; Vovides et al., 1993 ). Moreover, the influence of organ age on physiological traits has been reported for one

. Amer. Soc. Hort. Sci. 143 95 100 Zhang, D. Chen, Q. Liu, Q. Liu, F. Cui, L. Shao, W. Wu, S. Xu, J. Tian, D. 2019 Histological and cytological characterization of anther and appendage development in Asian lotus ( Nelumbo nucifera Gaertn.) Intl. J. Mol

a culture room under a 12-h photoperiod provided by cool-white fluorescent lamps with a photon flux density of 50 μmol·m −2 ·s −1 and temperature of 25 ± 2 °C. Histological observation. Explants with calluses or calluses with shoots at