In vitro propagation systems were developed for Carnegiea gigantea (Engelm.) Britt & Rose, Pachycereus pringlei (Berger) Britt & Rose and Stenocereus thurberi (Engelm.) Buxb, three North American species of columnar cacti. In vitro germinated seedlings were used as a source of explants. Multiple shoot formation from areoles was achieved for three types of explants (apical, lateral, and transverse) cultured on Murashige and Skoog (MS) basal media supplemented with 3% sucrose, 10 g·L-1 agar and various treatments with growth regulators. The highest shoot production efficiency for C. gigantea was obtained on transverse explants cultured on a medium with 2 mg·μmL-1 (8.87 μm) BA, where 5.3 shoots per explant were obtained. In P. pringlei and S. thurberi the best response was obtained using transverse explants on medium with 1 mg·L-1 (4.44 μm) BA (3.8 and 4.3 shoots per explant, respectively). Rooting of the in vitro generated shoots was achieved most efficiently on MS basal media with 3% sucrose, 10 g·L-1 agar and 1 mg·L-1 (4.9 μm) indole-3-butyric acid. Rooting frequencies were 92%, 88%, and 96% for C. gigantea, P. pringlei and S. thurberi, respectively, and the frequency of survival of the plants once transferred to soil was 86% on average. Chemical name used: benzyladenine (BA).
This study was conducted to investigate the possibility of obtaining plantlets via somatic embryogenesis and organogenesis as means of in vitro mass propagation in Allium victorialis var. platyphyllum Makino, one of the most popular wild vegetable plants in Korea. Shoots formed directly when bulb explants of A. victorialis were cultured on MS medium containing 0.2 mg·L–1 NAA and 2.0 mg·L–1 zeatin under 16 hours (light)/8 hours (dark) illumination. The use of leaf and shoot tip explants was not successful, largely due to explant senescence in the present of plant growth regulators. Embryogenic calli were obtained from the bulb explants of A. victorialis on MS medium supplemented with 0.2 mg·L–1 NAA, 0.2 mg·L–1 BAP, and 1.0 mg·L–1 picloram after 4–5 weeks of culture in the dark at 27°C. Upon transfer to shoot-induced MS medium containing 0.2 mg·L–1 NAA and 2.0 mg·L–1 zeatin, embryogenic calli gave rise to numerous somatic embryos, which subsequently developed into multiple shoots after 3 months of culture under 16 hours(light)/8 hours (dark) illumination. For root induction, regenerated shoots were transferred to MS medium added with 1.0 mg·L–1 NAA. Regenerants with well-developed roots were potted in an artificial soil mixture of vermiculite (1) and perlite (1).
Current-year branches in the uppermost whorl of unsheared balsam fir [Abies balsamea (L.) Mill.] trees were dipped 1, 2, or 3 times at weekly intervals in 0, 125, 250, 500, or 1000 ppm BA + 0.5% Buffer-X surfactant, starting every 2 weeks between 9 June and 18 Aug. 1982. BA applied once during June and early July increased lateral bud number and branch diameter in 1982, and increased lateral shoot production while decreasing lateral and whorl shoot elongation in 1983. The degree of response increased with increasing BA concentration, the greatest effect being obtained with 1000 ppm applied on 24 June when untreated branches had attained about 70% of their final length. Multiple applications were no more effective than a single application and caused phy to toxicity. The results indicate that BA application may be a useful method for increasing crown density in balsam fir Christmas trees.
A procedure was developed to regenerate plants via tissue culture from embryonic axes of mature avocado seeds. Explants were cultured in Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and naphthalene-acetic acid (NAA) or thidiazuron (TDZ) and NAA. Culture were kept in the dark for 7-10 days to reduce browning resulting from phenolic oxidation. Multiple shoots (5-8) were formed after transfer to light. Further multiplication were achieved using different combination of BA and NAA or TDZ and NAA. Shoots were cultured in MS supplemented with 2mg/l indolebutyric acid (IBA) for 2 weeks then transferred to MS supplemented with lg/l activated charcoal for root induction. Complete plants were obtained in vitro.
Numerous shooting and post-production techniques can be used to improve the quality of images used in horticultural publications. Certain lenses, lens attachments, and camera accessories are useful for enabling greater success in photographing plants. Small diffusers and reflectors allow the shooting of close-ups in the field, even when the sun is directly overhead. Shift lenses can be used to photograph trees at a closer distance without the extreme distortion of wide focal length lenses. Stitching of multiple images to produce panoramic shots can produce images with increased resolution, less distortion, and without the need for a wide-angle lens. Experiences with digital asset management management and post-production workflows are also presented.
This study examines the effect of multiple spray applications of Apogee on shoot growth and whole-canopy photosynthesis (WCPn) rate in young, bearing apple trees. Apogee increased fruit numbers and reduced shoot growth and inconsistently reduced leaf area but the reduction in photosynthetic area did not result in reduced WCPn or a detrimental effect on the fruit number:fruit size relationship. Since WCPn was not affected when leaf area was reduced by Apogee treatment, it suggests a greater photosynthetic efficiency of leaves on Apogee treated trees due to reduced shading. The use of Apogee for canopy management may produce a side-effect of increasing fruit set, which may be managed through a crop thinning program.
The dwarfing characteristics of St. Julien and Pixy rootstocks, measured by shoot growth, were evident with `AU-Amber' and `AU-Producer' plum (Prunus salicina Lindl.) scions. Dwarfing did not occur with `AU-Rubrum'. Trunk cross-sectional area (TCA) was reduced with `AU-Amber', `AU-Producer', and `AU-Rubrum' scions on St. Julien and Pixy rootstocks. After 3 years, tree survival was 94% for Lovell; 89%, Halford; 57%, Nemaguard; 75%, Nemared; 83%, St. Julien; and 47%, Pixy. Tree survivability was significantly lower on Nemaguard and Pixy rootstocks than on Lovell and Halford. Multiple regression of total shoot growth, TCA, and survivability against foliar nutrient content resulted in the following significant equations: 0.460Mg - 0.210Mn, 0.236B - 0.487Mn, and 0.359N + 0.398Ca - 0.267P - 0.360Fe for each, respectively. Growth, survivability, and foliar nutrient content are significantly affected by rootstock in plum production.
Micropropagation methods for six rockrose species (Cistus albidus L., C. clusii Dunal, C. ladanifer L., C. laurifolius L., C. psilosepalus L., and C. salvifolius L.) were established. Cultures, initiated from nodal segments of seedlings, were grown on MS medium, alone, or supplemented with 0.88 μm BAP or 0.93 μm Kin. Multiple shoot formation was obtained after the first subculture (30 days) from which new nodal segments were taken and grown on the same culture medium to maintain proliferation. Shoots obtained at the third subculture were rooted alone or supplemented with different concentrations of IBA. The plantlets of the six species, thereby obtained, were successfully acclimatized to ex vitro conditions. Chemical names used: 6-benzylaminopurine (BAP), indole-3-butyric acid (IBA), 6-furfurylaminopurine (Kin).
Guava (Psidium guajava L.), also called `apple of tropics,' is immensely nutraceutical and horticulturally important. Being a tropical plant, it cannot stand temperatures below 25° F and needs frost protection to grow in temperate regions. To adapt in cold climate, cold hardy guava cultivars are needed. Conventional ways are uneconomic in time and efforts. Still, transgenic plants developed using biotechnological approaches of tissue culture and rDNA technology, appear to have great potential. Thus, protocols for in vitro propagation of guava were developed via organogenesis and somatic embryogenesis using nodal explants from mature trees and young zygotic embryos, respectively. Nodal explants induced multiple shoots when cultured on MS medium fortified with KIN, BAP and Ad.S. Adding a (NO3)2 to medium was useful to prevent in vitro shoot tip browning of adventitious shoots. Rocker liquid culture greatly increased growth of multiple shoots compared to the agar-based medium. It appears to be a good tool for woody plant tissue culture. Induction of somatic embryos in guava was also achieved on MS medium supplemented with IAA auxin. About 80% to 90% somatic embryos germinated normally. To achieve Agro-bacterium-mediated gene transfer in guava, on-going co-cultivation of organogenic tissues of guava is to optimize protocols for freeze tolerance gene (CBF1, CBF2, CBF3) transfer. Plasmid vectors containing selectable markers (nptII gene for antibiotic selection and GUS reporter gene as scorable gene mediated selection), with CaMV 35S promoter gene has been introduced into guava tissues and the resultant plants showed antibiotic resistance. Details of the experimental procedures and up-to-date results will be discussed.
Bare-root 17.5-inch-tall (44.45-cm) `Sarah's Favorite' crapemyrtle (Lagerstroemia indica L.) liners were grown in #3 [2.75-gal (10.4-L)] black plastic containers and trained to one, three, or five trunks by one of two methods. Half of the plants were established from multiple liners with each trained to form one of the trunks. The others were established by planting a single liner in each container, pruning them back to within 2 inches (5.1 cm) from the substrate surface, and then training elongating buds or adventitious shoots to the desired number of trunks. Once plants reached a marketable size they were transplanted to a landscape for two growing seasons to determine the effects of the treatments on trunk survival or growth uniformity in the landscape. The study was replicated in time with containerized `Basham's Party Pink' crapemyrtle liners, but only grown in the field for 1 year. Growth and quality differences were minimal at the end of nursery production for either clone, thus favoring recommendation of whichever treatment would be most economical to produce the desired growth form. However, in the landscape phase, survival of `Sarah's Favorite' crapemyrtle and growth and uniformity of `Basham's Party Pink' crapemyrtle were greater for several growth measures when multiple trunks were produced by training stems of the same plant as opposed to planting multiple liners. Trunk survival was generally good for three or fewer trunks, but significant losses often occurred when the planting units had five trunks, especially when grown from multiple liners. Growth and survival differences among treatments were more pronounced with increasing trunk number and the longer the planting units were in the field (landscape).