Abstract
A protocol for in vitro propagation was developed with two dry bean (Phaseolus vulgaris L.) cultivars. Shoot cultures were initiated by placing seedling shoot tips (1.0 to 1.5 cm) on Murashige and Skoog (MS) medium in which the effects of kinetin and BA alone or in combination with IAA or NAA were examined with regard to shoot multiplication and root or basal callus formation. The combination of BA (3.0 mg liter–1) and NAA (0.1 mg·liter–1) was most effective in shoot multiplication. At high concentrations of BA or kinetin (>10 mg·liter–1), shoot production and internode elongation decreased markedly and rosette-like cultures with multiple buds developed. Shoots were rooted on basal MS medium. Ramets grew to maturity in the greenhouse or field and produced fertile flowers, pods, and seeds. Chemical names used: N-(2-furanylmethyl)-1H-purin-6-amine (kinetin); N-(phenylmethyl)-1H-purin-6-amine (BA); 1H-indole-3-acetic acid (IAA); 1-naphthaleneacetic acid (NAA).
In 1995, BAS-125W applied at 125 to 500 mg/liter 23 days after full bloom (AFB) to `Starkrimson Delicious'/MM 106 and MM111 reduced average shoot weight and length of the longest shoots in the top and scaffold limbs by 50% at the highest rate. The number of nodes on the lower 40 cm of each shoot was increased by 1.8 times by the growth retardant. The number of pruning cuts, pruning time, and pruning weight per tree was reduce by 30%, 20%, and 29%. Fruit diameter, color, soluble solids, starch, fruit weight, and fruit number per tree were not altered by BAS-125 W. Growth suppression appeared to be greater on trees with heavier crop loads. In 1996, BAS-125W applied at 250 mg/liter 8 days after full bloom was more effective than when applied 19 days AFB to `Starkrimson Delicious'/MM 106 and MM111. Multiple applications of two, three, and four sprays to the same trees at 3-week intervals further reduced shoot growth with each application. Four applications reduced shoot weight by 72%, shoot length by 60%, and basal shoot diameter by 25%, and the number of pruning cuts, pruning time, and pruning weight per tree was reduce by 75%, 55%, and 80%, respectively. Thinning activity of NAA, Sevin, or Accel was not affected by tank mixed sprays with BAS-125W when applied to Gala/M.27 trees 20 days AFB. Tank mixing BAS-125W with combinations of Vydate + Accel or Carbaryl + Accel + Oil did not alter fruit thinning of Fuji/M.27 (at 10 mm fruit diameter). In one experiment, BAS-125 may have potentiated thinning by ethephon and NAA 10 days AFB in another experiment. BAS-125 W sprays at petal fall + 1 and 2 weeks later significantly suppressed % infection by fireblight, Erwinia amylovora, in inoculated shoots. In addition, BAS-125W reduced canker length in the first-year growth in shoots inoculated 2 weeks after treatment.
Turfgrass is grown under extremely variable light intensities. This presents difficult management problems, and methods are needed to improve turf performance under variable shade conditions. Two experiments were conducted to determine the influence of trinexapac-ethyl (TE) on turf performance and physiological responses of `Diamond' zoysiagrass [Zoysia matrella (L.) Merr.] under several light intensities. In a polyethylene-roofed greenhouse, `Diamond' was sodded in 12 wooden boxes (1.2 × 1.2 × 0.16 m) (Expt. 1) and 18 fiber containers (55 × 38 × 12 cm) (Expt. 2). Treatments applied to boxes or containers included three levels of shade (40%, 75%, and 88%) with and without multiple TE applications at 48 g·ha-1 of active ingredient. Without TE treatment, vertical shoot growth increased linearly with increasing shade levels. Excessive shoot growth under 75% and 88% shade exacerbated energy depletion, as evidenced by the 45% and 67% lower rhizome mass and the 37% and 65% lower total nonstructural carbohydrate content (TNC), respectively, compared with turf under 40% shade. Trinexapac-ethyl reduced excessive vertical shoot growth and increased rhizome mass and TNC. Mean turf quality was increased by 0.7 and 1.4 units for turf receiving multiple TE applications under 75% and 88% shade, respectively. Trinexapac-ethyl did not increase turf quality or TNC under 40% shade. Canopy photosynthetic rate (Pn) was not affected 4 weeks after the initial TE treatment under any shade level. However, 34 weeks after the initial TE treatment a 50% higher Pn was observed for turf treated with TE under 88% shade, possibly because of higher tiller density. Repeated TE application increased turf quality and provided more favorable physiological responses (such as TNC and Pn) under 75% and 88% shade, where conditions favored vertical shoot growth. However, little or no improvement in turf quality was observed under 40% shade, where conditions favored slow vertical shoot growth. Chemical name used: 4-(cyclopropyl-α-hydroxy-methylene)-3,5-dioxo-cyclohexanecarboxylic acid ethyl ester (trinexapac-ethyl).
Fifty-four out of 67 species of bamboo tested were successfully propagated in vitro. For nearly every species, multiple shoots were produced from axillary buds on stem node segments cultured on Murashige and Skoog medium containing BA. In a very few species plants could be regenerated adventitiously from callus. This method of propagation was not very efficient or reliable. Rooting occurred in media containing NAA at 2.7 to 5.4 μM. Several species could be stored in vitro on half-strength medium at room temperature > 15 months without transfer. Chemical names used: N6-benzylamino purine (BA); napthyleneacetic acid (NAA).
Abstract
Morphological changes during development of cultured citrus explants (Citrus sinensis (L.) Osbeck cv. Shamouti) were observed with a scanning electron microscope (SEM). Prophylls of resting buds, covered with epidermal hairs, were closely appressed until the growth of a new shoot; they then expanded. The addition of 10-5 m 6-benzylaminopurine to the medium resulted in the formation of several adventitious buds, surrounded by multiple prophylls, in the axil of the petiole. Abscission of the petiole from the explant involved formation of a separation zone with no evidence of new dividing cells, or active cell division and formation of callus tissue in the abscission zone.
A mixture of C8/C10 fatty acid methyl esters (FAME) when applied directly and exclusively to leaf axils of greenhouse-grown tomato (Lycopersicon esculentum Mill.) significantly inhibited side shoot development. Plants grown in a single cluster production system in winter produced 8.9 side shoots/plant, whereas those treated with C8/C10 FAME 45 days after sowing, produced only 0.7 side shoots/plant. Total pruning weight of the side shoots was reduced from 40.2 g/plant to 1.3 g/plant. Fruit yield increased 14% with C8/C10 FAME treatment and there was an increase in the harvest index from 0.63 to 0.70. For a spring crop, in which average daily irradiance was more than twice that in winter, overall yield increased 70% when compared to the winter crop. As in winter, side shoot number and side shoot weight/plant were significantly reduced by C8/C10 FAME, but there was no difference in crop yield between C8/C10 FAME and untreated plants. In both winter and spring, untreated plants required hand pruning three times during the production period, whereas C8/C10 FAME-treated plants were pruned only once at the time of application. A C8/C10 free fatty acid (FA) mixture was also applied to one and two-cluster plants with similar results. In the multiple cluster system, application of the C8/C10 FA mixture instead of side shoot pruning reduced plant height and increased yield from 6.4 to 7.4 kg/plant. C8/C10 FA or C8/C10 FAME treatment could be a useful labor saving strategy in greenhouse tomato production and may increase crop yield under conditions in which assimilates may be limited by environmental factors, or as a result of a high level of competition from other fruits or shoots.
In vitro propagation systems were developed for Carnegiea gigantea (Engelm.) Britt & Rose, Pachycereus pringlei (Berger) Britt & Rose and Stenocereus thurberi (Engelm.) Buxb, three North American species of columnar cacti. In vitro germinated seedlings were used as a source of explants. Multiple shoot formation from areoles was achieved for three types of explants (apical, lateral, and transverse) cultured on Murashige and Skoog (MS) basal media supplemented with 3% sucrose, 10 g·L-1 agar and various treatments with growth regulators. The highest shoot production efficiency for C. gigantea was obtained on transverse explants cultured on a medium with 2 mg·μmL-1 (8.87 μm) BA, where 5.3 shoots per explant were obtained. In P. pringlei and S. thurberi the best response was obtained using transverse explants on medium with 1 mg·L-1 (4.44 μm) BA (3.8 and 4.3 shoots per explant, respectively). Rooting of the in vitro generated shoots was achieved most efficiently on MS basal media with 3% sucrose, 10 g·L-1 agar and 1 mg·L-1 (4.9 μm) indole-3-butyric acid. Rooting frequencies were 92%, 88%, and 96% for C. gigantea, P. pringlei and S. thurberi, respectively, and the frequency of survival of the plants once transferred to soil was 86% on average. Chemical name used: benzyladenine (BA).
This study was conducted to investigate the possibility of obtaining plantlets via somatic embryogenesis and organogenesis as means of in vitro mass propagation in Allium victorialis var. platyphyllum Makino, one of the most popular wild vegetable plants in Korea. Shoots formed directly when bulb explants of A. victorialis were cultured on MS medium containing 0.2 mg·L–1 NAA and 2.0 mg·L–1 zeatin under 16 hours (light)/8 hours (dark) illumination. The use of leaf and shoot tip explants was not successful, largely due to explant senescence in the present of plant growth regulators. Embryogenic calli were obtained from the bulb explants of A. victorialis on MS medium supplemented with 0.2 mg·L–1 NAA, 0.2 mg·L–1 BAP, and 1.0 mg·L–1 picloram after 4–5 weeks of culture in the dark at 27°C. Upon transfer to shoot-induced MS medium containing 0.2 mg·L–1 NAA and 2.0 mg·L–1 zeatin, embryogenic calli gave rise to numerous somatic embryos, which subsequently developed into multiple shoots after 3 months of culture under 16 hours(light)/8 hours (dark) illumination. For root induction, regenerated shoots were transferred to MS medium added with 1.0 mg·L–1 NAA. Regenerants with well-developed roots were potted in an artificial soil mixture of vermiculite (1) and perlite (1).
Zygotic embryo explants of grape cultivar AXR#1 were isolated from maw-e seeds and cultured on medium supplemented with naphthoxy acetic acid beta-(NOA) and benzylaminopurine (BA). Embryo explants dedifferentiated to form embryogenic callus. Globular stage embryos were visible in 9-10 months. On transfer 10 a growth regulator free medium supplemented with charcoal these globular embryos underwent further stages of embryo development. In a period of 30-40 days embryogenic tissues turned into clumps of somatic embryos displaying different stages of development Cotyledonary stage embryos were separated and transferred to basal medium. These embryos developed into complete plants. Cold and desiccation treatment of somatic embryos significantly enhanced the rate of plant conversion. Hypocotyl segments of elongated somatic embryos were good source explant for induction of shoot organogenesis. The hypocotyl-length and the proximity to-shoot-apex were found to influence the rate of shoot induction from hypotyl segments. Multiple shoot complexes which formed on hypocotyl segments were separated and individual shoots were grown on a root induction medium resulting in complete plant development. The possibility of both embryogenic and organogenic modes of plant regeneration make somatic embryos a highly versatile explant source for experiments on genetic manipulation.
Abstract
Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] plantlets, micropropagated from axillary buds of nodal segments from invigorated stems and adventitious buds from young needles of two 60-year-old trees, have been established in soil. Stem tips with half-pruned-back needles from the lower crown, when placed on DCR medium, sprouted buds in 90% of explants. Shoots from bud sprouts were plagiotropic when collected from explants of shoots with a horizontal and downward growth habit. By contrast, upright growth of micropropagated shoots was observed in all explants from branches with an upright habit. After four subcultures (3- to 4-week intervals on 0.5 mg·liter−1 BA in DCR), sprouted shoots of nodal segments from all expiant sources produced four to six multiple buds. With excised needles of sprouted buds, numerous (> 10) adventitious buds formed after 11 to 12 weeks on needle surfaces after the fifth subculture on DCR with 0.5 mg·liter BA. All adventitious and axillary buds elongated on 0.5 DCR lacking plant growth regulators. Rooting of the elongated shoots was induced in 20% of the explants with 0.2 mg·liter−1 each of NAA and IBA. In all instances, buds programmed for the development of female reproductive cones continued their phase-specific development even under conditions conducive to rejuvenation of vegetative tissues. Chemical names used: N-(phenylmethy)-1H-purin-6-amine (BA); 1-naphthaleneacetic acid (NAA); 1H-indole-3-butyric acid (IBA).