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°C for 20 min. Histology. Embryogenic tissues were collected during embryonic development of somatic embryos and were fixed in FAA solution (formalin:acetic acid:absolute ethanol:distilled water of 5:5:45:45, v/v/v/v) for 24 h at room temperature for

. Histological study. Samples were fixed in Formalin acetic acid alcohol (FAA; formaline, acetic acid, and 70% ethanol as 5:5:90), dehydrated with Deshidratante histológico (Biopur SRL, Rosario, Argentina), embedded in paraffin wax (Paraplast Plus ® ; Sigma

explants per petri dish in Expts. 1 and 2, but four nodal explants per dish in Expt. 3. Expt. 3 was repeated three times. Histological observation. Samples collected from different culture periods of nodal explants were taken weekly and fixed in FAA

such as roses. Luvisi et al. (p. 1037) report a new method of microchip insertion into rose canes of less than 10 mm diameter, such as those typically found in nurseries. In order to evaluate the effects of methods of microchip insertion, histological

histology measurements between genotypes, all except ‘NY 54’, ‘Bing’, and ‘Regina’ at MSU-CHES were mature (>20 years) trees grafted on P. avium seedling rootstock and trained to an open center. ‘NY 54’, ‘Bing’, and ‘Regina’ at MSU-CHES were younger (3

other pomegranate products. The morphological and histological characterization of pomegranate flowers has been recently described ( Wetzstein et al., 2011a ). Both hermaphroditic (bisexual) and functionally male flowers are on the same plant, a

cotyledon explants showed the same time course of morphological and histological changes as previously described ( Ermel et al., 2000 ; Gutmann et al., 1996 ; Jay-Allemand et al., 1991 ). The distribution of IAA in the walnut cotyledon was revealed by

) under illumination (30–34 μmol·m −2 ·s −1 ) with 12-h photoperiod and a temperature of 25 ± 2 °C. Histological study of somatic embryo development. The embryonic calli on m m S were fixed in FAA solution (formalin: acetic acid: 70% ethanol = 1:1:18 v

stained with toluidine blue O (for general histology), phloroglucinol:HCl (for lignified cell walls), I 2 KI (for starch), and Sudan IV (for cutinized and suberized cell walls) ( Ruzin, 1999 ). Anatomical images were captured with a digital camera on a

) Histological section (LS) of a primary androgenic (pe) embryo with two generations of secondary somatic embryos at different stages, originating from the tissue at cotyledon. Bar = 400 μm. ( F ) Histological section (LS) of primary androgenic embryo (pe