°C for 20 min. Histology. Embryogenic tissues were collected during embryonic development of somatic embryos and were fixed in FAA solution (formalin:acetic acid:absolute ethanol:distilled water of 5:5:45:45, v/v/v/v) for 24 h at room temperature for
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Yuyu Wang, Faju Chen, Yubing Wang, Xiaoling Li, and Hongwei Liang
Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut
. Histological study. Samples were fixed in Formalin acetic acid alcohol (FAA; formaline, acetic acid, and 70% ethanol as 5:5:90), dehydrated with Deshidratante histológico (Biopur SRL, Rosario, Argentina), embedded in paraffin wax (Paraplast Plus ® ; Sigma
Jin Cui, Juanxu Liu, Min Deng, Jianjun Chen, and Richard J. Henny
explants per petri dish in Expts. 1 and 2, but four nodal explants per dish in Expt. 3. Expt. 3 was repeated three times. Histological observation. Samples collected from different culture periods of nodal explants were taken weekly and fixed in FAA
such as roses. Luvisi et al. (p. 1037) report a new method of microchip insertion into rose canes of less than 10 mm diameter, such as those typically found in nurseries. In order to evaluate the effects of methods of microchip insertion, histological
James W. Olmstead, Amy F. Iezzoni, and Matthew D. Whiting
histology measurements between genotypes, all except ‘NY 54’, ‘Bing’, and ‘Regina’ at MSU-CHES were mature (>20 years) trees grafted on P. avium seedling rootstock and trained to an open center. ‘NY 54’, ‘Bing’, and ‘Regina’ at MSU-CHES were younger (3
Hazel Y. Wetzstein, Weiguang Yi, Justin A. Porter, and Nadav Ravid
other pomegranate products. The morphological and histological characterization of pomegranate flowers has been recently described ( Wetzstein et al., 2011a ). Both hermaphroditic (bisexual) and functionally male flowers are on the same plant, a
Ningguang Dong, Qingmin Wang, Junpei Zhang, and Dong Pei
cotyledon explants showed the same time course of morphological and histological changes as previously described ( Ermel et al., 2000 ; Gutmann et al., 1996 ; Jay-Allemand et al., 1991 ). The distribution of IAA in the walnut cotyledon was revealed by
Yihui Cui, Peng Zhao, Hongqiang An, Nan Lv, Zifeng Zhang, Wei Pei, and Wanjun Wang
) under illumination (30–34 μmol·m −2 ·s −1 ) with 12-h photoperiod and a temperature of 25 ± 2 °C. Histological study of somatic embryo development. The embryonic calli on m m S were fixed in FAA solution (formalin: acetic acid: 70% ethanol = 1:1:18 v
Jack B. Fisher, Anders Lindström, and Thomas E. Marler
stained with toluidine blue O (for general histology), phloroglucinol:HCl (for lignified cell walls), I 2 KI (for starch), and Sudan IV (for cutinized and suberized cell walls) ( Ruzin, 1999 ). Anatomical images were captured with a digital camera on a
Dušica Ćalić, Nina Devrnja, Jelena Milojević, Igor Kostić, Dušica Janošević, Snežana Budimir, and Snežana Zdravković-Korać
) Histological section (LS) of a primary androgenic (pe) embryo with two generations of secondary somatic embryos at different stages, originating from the tissue at cotyledon. Bar = 400 μm. ( F ) Histological section (LS) of primary androgenic embryo (pe