A standardized screening procedure for tolerance to bicarbonate-induced Fe chlorosis using a commercial fertilizer mix (Plantex) as the nutrient source, high solution pH (8.5) and 1.5 m m bicarbonate to simulate a calcareous soil situation was used with a 1 vermiculite:1 perlite (v/v) support media, small pots and topping (pinching back the tops of shoots). The tolerance level of peach [Prunus persica (L.) Batsch] rootstock could be assessed by leaf visual-chlorosis ratings and Spad-502 chlorophyll readings instead of extractable leaf-chlorophyll concentration or plant Fe concentration. Although most of the tolerant genotypes had almond [P. amygdalus (Mill.) D.A. Webb] in their parentage, a few peaches (`Swat', NJ672281007) showed high to moderate levels of tolerance.
Yan Shi and D. H. Byrne
John M. Ruter and Dewayne L. Ingram
Plants of `Rotundifolia' holly (Ilex crenata Thunb.) were grown for 3 weeks with root zones at 30,34,38, or 42C for 6 hours daily to evaluate the effects of supraoptimal root-zone temperatures on various photosynthetic processes. After 3 weeks, photosynthesis of plants grown with root zones at 38 or 42C was below that of plants grown at 30 or 34C. Chlorophyll and carotenoid levels decreased while leaf soluble protein levels increased as root-zone temperature increased. Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) activity per unit protein and per unit chlorophyll responded quadratically, while RuBisCO activity per unit fresh weight increased linearly in response to increasing root-zone temperature. Results of this study suggest that `Rotundifolia' holly was capable of altering metabolism or redistributing available assimilates to maintain CO2 assimilation rates in response to increasing root-zone temperatures.
John E. Erwin and Gerald Pierson
Lypcopersicum esculentum cv `Money Maker' seeds were germinated at constant 20C. Three days after germination seedlings were randomly divided into 3 groups and placed into 3 growth chambers maintained at 23/17, 20/20, or 17/23C (day/night temperature) (DT/NT). Irradiance and photoperiod were maintained at 250 μmol s-1 m-2 and 12 hrs, respectively. At the 2 leaf stage, plants in each chamber were divided into 3 groups of 3 plants each to receive a growth regulator treatment. Growth regulator treatments consisted of spray applications of either ancymidol (52ppm), GA3 (12ppm), or water applied every 3 days for 21 days. Measurements were taken on internode length and chlorophyll content after 21 days. Internode length increased as the difference (DIF) between DT and NT increased (DT-NT). Exogenous applications of GA3 overcame inhibition of stem elongation resulting from a -DIF environment. Application of ancymidol did not significantly decrease stem elongation in a -DIF environment. Temperature regime had a significant impact on chlorophyll content per mg dry weight. In contrast, growth regulator applications had a significant impact on chlorophyll content cm-2. There was no significant impact of either temperature regime or growth regulator treatment on the chlorophyll a/b ratio.
Jun Song, Lihua Fan, Charles F. Forney, and Michael A. Jordan
Ethanol production and chlorophyll fluorescence were measured as signals of freezing and heat stress in apple fruit. `Cortland' and `Jonagold' apples were held at –8.5 °C for 0, 6, 12 or 24 h (freezing treatments), or at 46 °C for 0, 4, 8 or 12 h (heat treatments). Following treatments, fruit were stored at 0 °C and evaluated after 0, 1, 2, or 3 months. Following storage, fruit samples were kept for 12 h at 20 °C and then analyzed for ethanol production, chlorophyll fluorescence, and visible injury. Severity of flesh browning increased with increasing treatment time for both freezing and heat treatments. Freezing for 24 h and heating for 12 h caused severe flesh browning in both cultivars. Severity of heat-induced browning increased during storage. Increases in ethanol production were apparent 12 h following treatments and reflected the degree of stress-induced fruit injury. After 2 months of storage, ethanol concentrations peaked and were as much as 400-fold greater than that of controls. These stress treatments also reduced ethylene production and chlorophyll fluorescence. The degree of increase in stress-induced ethanol production and decrease in chlorophyll fluorescence correlated with stress-induced injury and could be used to predict the severity of injury that develops during storage. Other volatile production and their relationship to fruit stress will also be discussed.
Jun Song, Lihua Fan, Charles F. Forney, and Michael A. Jordan
Volatile emissions and chlorophyll fluorescence were investigated as potential signals of heat injury for apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] fruit. `McIntosh', `Cortland', `Jonagold', and `Northern Spy' apples were exposed to 46 °C for 0, 4, 8, or 12 hours (heat treatments). Following treatments, fruit were kept at 20 °C and evaluated after 1, 2, 4, or 7 days. Heat treatments induced volatile production including ethanol and ethyl acetate. The 8 and 12 hours heat treatments increased ethanol and ethyl acetate production in all four cultivars by as much as 170- and 11-fold, respectively, 1 day after treatments. Heat treatments also reduced ethylene production and chlorophyll fluorescence. Heat for 12 hours caused serious flesh browning. Among the cultivars investigated, `Northern Spy' and `McIntosh' were most susceptible to heat stress based on the degree of flesh browning. Correlation coefficients of heat stress induced ethanol emission and chlorophyll fluorescence with flesh browning were 0.82 and -0.66, respectively. The nondestructive measurements of ethanol emission and chlorophyll fluorescence have potential to identify stressed fruit with reduced quality or compromised storage life.
Mack Thetford, Stuart L. Warren', Frank A. Blazich, and Judith F. Thomas
Uniconazole was applied as a foliar spray at 0, 90, 130, 170, or 210 ppm to rooted stem cuttings of `Spectabilis' forsythia (Forsythia xintermedia Zab.) potted in calcined clay. Uniconazole resulted in higher total leaf chlorophyll (chlorophyll + chlorophyll,) concentration and a decreased ratio of chlorophyll a: b. Stomata1 density of the most recently matured leaves increased linearly with increasing uniconazole concentration 40, 60, and 100 days after treatment (DAT). The number of stomata per leaf (stomata1 index) increased linearly with increasing concentration of uniconazole throughout the initial 100 DAT. Uniconazole suppressed stomata1 length at all sampling dates and the level of suppression increased with increasing concentration of uniconazole from 20 to 100 DAT. Stomata1 width was suppressed by uniconazole at 40 DAT. Leaves developed after uniconazole application had higher levels of net photosynthesis when measured 55, 77, and 365 DAT. Stomata1 conductance for uniconazole-treated plants was higher compared to nontreated control (0 mg·liter-1) plants when measured 49, 55, 77, and 365 DAT. Initiation of secondary xylem for stem tissues of uniconazole-treated plants was suppressed and expansion of xylem vessel length and width was less. Secondary phloem tissues of stems from uniconazole-treated plants contained larger numbers of phloem fibers having smaller cross sectional areas than phloem fibers of controls. Chemical name used: (E)-1-(p-Chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-01 (uniconazole).
Renae Moran, Jennifer DeEll, and Cindy B.S. Tong
et al., 2005 ). Ground color changes and chlorophyll breakdown occur in the peel during ripening ( Knee, 1972 ), but this can be subjective in the case of ground color and difficult to measure on large numbers of fruit in the case of chlorophyll. The
Y.C. Li, A.K. Alva, D.V. Calvert, and M. Zhang
Rapid and accurate diagnosis of plant nutrient deficiency is critical for growers to use fertilizer economically and to minimize environmental concern. The objective of this study was to evaluate the use of leaf chlorophyll meter (SPAD-502) to predict nitrogen status and yield response of grapefruit trees. The study includes two irrigation treatments, three fertilizer sources, and four fertilizer rates. No significant differences in SPAD readings and leaf N were found between two irrigation treatments. Correlations between SPAD readings and leaf N were higher for the spring flush (r = 0.61) than that for summer flush (r = 0.49). There were poor relations between SPAD meter readings and the extractable chlorophyll content in the spring flush on a dry-weight basis (r = 0.25). Higher correlations between SPAD meter readings and N rates or fruit yields than between leaf N concentrations and N rates or fruit yield.
Sean M. Westerveld, Alan W. McKeown, Mary Ruth McDonald, and Cynthia D. Scott-Dupree
Nutrient management legislation has prompted an evaluation of alternative nitrogen (N) management techniques. SPAD (Soil Plant Analysis Development) chlorophyll and Cardy nitrate (NO3 -) meters were evaluated for their potential as tissue nitrogen tests in cabbage (Brassica oleracea var. capitata), onions (Allium cepa), and carrots (Daucus carota subsp. sativus). Cabbage, carrots, and onions were grown on both organic and mineral soils in Ontario, Canada in 2000 and 2001. Nitrogen was applied at five rates to cabbage and carrots and three rates to onions ranging from 0 to 200% of current provincial recommended N rates. In an additional treatment, 50% of the recommended rate was applied preplant and sidedress N applications of 40 kg·ha-1 (35.7 lb/acre) were applied when SPAD chlorophyll meter readings fell below 95 (2000) and 97% (2001) of the highest N rate treatment. Yields were generally unaffected by N rate, except in cabbage in 2000, suggesting adequate N was present in most treatments. SPAD chlorophyll meter readings were highly variable among soil types, cultivars, and years. Chlorophyll readings reached a plateau in adequately fertilized crops in many instances. Cardy readings were less variable among soil types, cultivars, and years. The relationship between N rate and sap NO3-N concentration was generally linear. The results suggest that the use of a well-fertilized reference plot is most appropriate for the SPAD meter on these vegetable crops, while the use of region-specific critical NO3-N concentrations was most appropriate for the Cardy NO3-meter. Both meters would be cost advantageous when over 500 samples are tested. The meters were generally easy to use, except for the SPAD meter on carrots. The meters have potential for N management of vegetable crops under Ontario growing conditions.
Charles F. Forney, Michael A. Jordan, Kumudini U.K.G. Nicholas, and Jennifer R. DeEll
Use of volatile emissions and chlorophyll fluorescence as indicators of freezing injury were investigated for apple fruit (Malus ×domestica Borkh.). `Northern Spy' and `Delicious' apples were kept at -8.5 °C for 0, 6, or 24 h, and then at 20 °C. After 1, 2, 5, and 7 d at 20 °C, fruit were analyzed for firmness, skin and flesh browning, soluble solid content, titratable acidity, ethanol, ethyl acetate, ethylene, respiration rate, and chlorophyll fluorescence. Freezing caused skin and flesh browning and a loss of fruit firmness, which was greater in `Northern Spy' than in `Delicious'. In `Northern Spy' fruit subjected to the freezing treatments, ethanol and ethyl acetate concentrations were as much as 37- and 300-fold greater, respectively, than in control fruit. `Delicious' fruit showed similar patterns of ethanol and ethyl acetate increase, but of lower magnitude, as a result of freezing. Higher fruit respiratory quotients were associated with increased ethanol and ethyl acetate concentrations. Ethylene production and chlorophyll fluorescence of fruit were reduced by freezing.