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remaining pellet was again resuspended in 40 mL of HCl, pH 2.5, and incubated for 90 min at 60 °C. The extract was centrifuged and filtered to obtain the acid-soluble pectin (ASP). The remaining pellet was resuspended in 40 mL of 0.05 m NaOH and incubated

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Instruments, Wilmington, DE) 5 min at 10,000 g n at 4 °C. Supernatant (3 mL) was removed to a test tube, to which 1 mL derivatizing solution, 1,2-benzenediamine dihydrochloride, was added (final Ph, 2.20–2.45), vortexed for 5 s, filtered through a 45-μm

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20 m m potassium phosphate (pH 2.5 by phosphoric acid) at a flow rate of 1 mL/min and were detected at 210 nm. L-Proline (Sigma) dissolved in the 20 mm potassium phosphate solution was used to calibrate the standard curve. The amount of proline in

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with the producers reporting research on nutrient solution formulations (mean = 3.1 ± 1.0) and nutrient solution pH (2.8 ± 1.0) being slightly to very beneficial ( Fig. 6 ). Additionally, with the majority of producers surveyed monitoring and

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automated titrimeter and electrode standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was determined by titrating 0.1 N sodium hydroxide to 6 g of juice diluted with 50 mL of deionized, degassed water to an endpoint of pH 8.2, with

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monobasic (KH 2 PO 4 , 0.5%, w/v) at pH 2.5 with metaphosphoric acid (HPO 3 , 0.1%, w/v). The retention time of the AA peak was 2.5 min. After comparison of retention time with the AA standard, the peak was identified. The amount of total AA content in

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thawed, placed in cheesecloth, and squeezed to extract the juice from the berries. Titratable acidity and pH were measured by an 877 Titrino Plus (Metrohm AG, Herisau, Switzerland) standardized to pH 2.0, 4.0, 7.0, and 10.0 buffers. Titratable acidity was

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Detector set at 210 nm using an X-bridge C18 column (4.6 × 250 mm, 5 μm, Waters Corporation) operated at 30 °C. The elution solvent was 0.01 mol·L −1 sulfuric acid (pH 2.6) passed at a rate of 0.5 mL·min −1 . The quantity and type of organic acids were

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  =   0.8780   ×   Δ pH 3 +   1.3369   ×   Δ pH 2 +   9.9856   ×   Δ pH −   0.2569 [1] Plant growth was measured as root and shoot dry weight gain during the experiment. Root and shoot tissue from seedlings and from final replicates was oven-dried for 48 h

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of 5 m m potassium phosphate monobasic (KH 2 PO 4 ), pH 2.65, with 0.1% of formic acid (solution A) and methanol with 0.1% of formic acid (solution B). The linear gradient of the mobile phase was programmed as follows: 5% to 15% B for 1 min, followed

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