Plant growth regulators (PGRs) are chemicals used on a wide range of horticultural crops. These exogenous chemicals, similar to endogenous plant hormones, regulate plant development and stimulate a desired growth response, such as control of plant height. One such PGR is abscisic acid (ABA), which has been used effectively to improve fruit quality, specifically sugars and phytonutrients. The purpose of this study was to examine the effects of exogenous applications of ABA on tomato (Solanum lycopersicum) fruit quality, such as carotenoids, soluble sugars and organic acids, and ABA on tomato leaf chlorophylls and carotenoids. Furthermore, this study compared how ABA and calcium (Ca) treatments together affect fruit quality and whether there are added benefits to treating plants with both simultaneously. ABA treatments proved effective in increasing tomato fruit soluble sugars and decreasing organic acid concentrations. This study demonstrated that ABA is a viable PGR to significantly improve tomato fruit quality, specifically pertaining to carotenoids, soluble sugar, and organic acid concentrations.
T. Casey Barickman, Dean A. Kopsell, and Carl E. Sams
Enoc Barrera-Aguilar, Luis A. Valdez-Aguilar, Ana M. Castillo-González, Andrew D. Cartmill, Donita L. Cartmill, Edilberto Avitia-García, and Luis Ibarra-Jímenez
The present study was conducted to determine the critical optimum and toxic concentrations of potassium (K) using segmented analysis and its relationship with some physiological, anatomical, and nutritional responses to increasing K in hydroponically grown Lilium sp. L. cv. Arcachon. Plants were fertigated with nutrient solutions containing K (Kext) at 0, 2.5, 5.0, 7.5, 12.5, 17.5, 22.5, and 30 mmol·L−1. Maximum flower diameter occurred when, on a dry mass basis, shoot K (Kint) ranged from 504 to 892 mmol·kg−1; however, a lower Kint was required to obtain maximum biomass accumulation and shoot length (384 and 303 mmol·kg−1, respectively). Potassium increased in all plant organs as K in the nutrient solution increased. Nitrogen increased in young leaves and magnesium (Mg) decreased as Kext increased. Concentrations of Kext from 5 to 17.5 mmol·L−1 increased the size of chlorenchyma and occlusive cells; however, metaxylem vessels were unaffected. Net photosynthetic rate was higher in young leaves, whereas water potential increased in both young and mature leaves when Kext was greater than 22.5 mmol·L−1. Critical concentrations varied according to the growth parameter. Optimum Kint ranged from 303 to 384 mmol·kg−1 for vegetative parts, whereas parameters related with flower growth ranged from 427 to 504 mmol·kg−1. Concentration of 504 mmol·kg−1 Kint was associated with optimum growth for all the parameters assessed, whereas a Kint greater than 864 mmol·kg−1 was associated with a decline in growth; thus, these concentrations were considered as the critical optimum and critical toxicity levels, respectively. The optimum and toxicity critical Kint were estimated when Kext in the nutrient solutions was 5.6 and 13.6 mmol·L−1, respectively.
Youbin Zheng, Linping Wang, and Mike Dixon
Copper (electrolytically generated or from cupric sulfate) is increasingly used to control diseases and algae in the greenhouse industry. However, there is a shortage of information regarding appropriate management strategies for Cu2+ (Cu) in greenhouse hydroponic production. Three greenhouse studies were conducted to examine the growth and yield responses of sweet pepper (Capsicum annuum L., Triple 4, red) to the application of Cu in hydroponic production systems. In the first two experiments, plants were grown on rockwool and irrigated with nutrient solutions containing Cu at concentrations of 0.05, 0.55, 1.05, 1.55, and 2.05 mg·L–1. Copper treatments were started either when plants were 32 days old and continued for 4 weeks, or when plants were 11 weeks old and continued for 18 weeks, respectively. In the third experiment, roots of solution cultured pepper seedlings were exposed to Cu (1.0, 1.5, and 2.0 mg·L–1) containing nutrient solutions for 2 hours per day for 3 weeks. Higher Cu treatment initialized when plants were 32 days old significantly reduced plant leaf number, leaf area, leaf biomass, specific leaf area, stem length and shoot biomass. The calculated Cu toxicity threshold was 0.19 mg·L–1. However, when treatment initialized at plants were 11 weeks old, Cu did not have significant effects on leaf chlorophyll content, leaf area or specific leaf area. Copper started to show significant negative effects on leaf biomass and shoot biomass at 1.05 mg·L–1 or higher levels. Copper treatments did not have any significant effect on fruit number, fresh weight or dry weight. Under all the Cu levels, fresh fruit copper contents were lower than 0.95 mg·kg–1 which is below the drinking water standard of 1.3 mg·kg–1. Seedling growth was significantly reduced by exposing roots to Cu (≥1.0 mg·L–1) containing solutions even for only 2 h·d–1.
Robert C. Hochmuth and George J. Hochmuth
The evolution of plastic uses (excluding glazing) in the production of greenhouse vegetables is presented. Plastics are used in almost every aspect of crop production, including providing a barrier to the soil, lining crop production troughs, holding soil and soilless media, and providing a nutrient film channel. Irrigation systems have become very elaborate, with various plastic products used to transport water and nutrients and to provide a means of emitting nutrient solution to the crop. The greenhouse environment is managed from several plastic components, including air distribution tubes, shade materials, and energy curtains. Plastics are now common in greenhouse vegetable crop training, insect monitoring, postharvest handling, storage, and marketing.
Rhuanito S. Ferrarezi, Alan L. Wright, Brian J. Boman, Arnold W. Schumann, Fred G. Gmitter, and Jude W. Grosser
Completely enclosed screen houses can physically exclude contact between the asian citrus psyllid [ACP (Diaphorina citri)] and young, healthy citrus (Citrus sp.) trees and prevent huanglongbing (HLB) disease development. The current study investigated the use of antipsyllid screen houses on plant growth and physiological parameters of young ‘Ray Ruby’ grapefruit (Citrus ×paradisi) trees. We tested two coverings [enclosed screen house and open-air (control)] and two planting systems (in-ground and container-grown), with four replications arranged in a split-plot experimental design. Trees grown inside screen houses developed larger canopy surface area, canopy surface area water use efficiency (CWUE), leaf area index (LAI) and LAI water use efficiency (LAIWUE) relative to trees grown in open-air plots (P < 0.01). Leaf water transpiration increased and leaf vapor pressure deficit (VPD) decreased in trees grown inside screen houses compared with trees grown in the open-air plots. CWUE was negatively related to leaf VPD (P < 0.01). Monthly leaf nitrogen concentration was consistently greater in container-grown trees in the open-air compared with trees grown in-ground and inside the screen houses. However, trees grown in-ground and inside the screen houses did not experience any severe leaf N deficiencies and were the largest trees, presenting the highest canopy surface area and LAI at the end of the study. The screen houses described here provided a better growing environment for in-ground grapefruit because the protective structures accelerated young tree growth compared with open-air plantings while protecting trees from HLB infection.
Pai-Tsang Chang and William M. Randle
Onion is classified as a salt-sensitive crop, though it is found in production on saline soils around the world. While onion flavor intensity has been studied in response to various growing conditions, little is known about its response to salt stress. To understand if NaCl affects growth, flavor development, and mineral content in onion, `Granex 33' plants were grown to maturity with six different concentrations of NaCl ranging from 0 (control) to 125 mm in nutrient solutions. NaCl affected onion fresh weight and altered onion flavor intensity and quality. Plants did not survive the 125 mm NaCl treatments and are not included in the results. As bulb Na+ and Cl- content increased in response to increasing NaCl concentrations, leaf and bulb fresh weight of mature plants decreased. Total bulb S content also decreased with increasing NaCl solution concentrations, while bulb SO4 2- content increasing linearly, indicating that less S was entering the S metabolic stream. Though bulb soluble solids content was not influenced by NaCl concentrations, pungency increased, but only at the highest NaCl concentration. Total flavor precursors and methyl cysteine sulfoxide content increased in response to NaCl, but only at the 100 mm treatment. 1-Propenyl cysteine sulfoxide was generally unresponsive to the salt treatment. Propyl cysteine sulfoxide content decreased then increased in responses to increasing NaCl levels, but was found as a minor flavor precursor. Peptide intermediates measured in the pathway leading to 1-propenyl cysteine sulfoxide and propyl cysteine sulfoxide decreased linearly with increasing NaCl exposure. While NaCl affected onion flavor in this study, severe reductions in growth would prevent onion production under similar saline conditions. For practical purposes, the effects of NaCl on flavor are, therefore, minimal.
B.K. Duffy and G. Défago
Host nutritional variables were evaluated for their effects on the severity of crown and root rot of tomato caused by Fusarium oxysporum f.sp. radicis-lycopersici. Tomato (Lycopersicon esculentum Mill.) seedlings (cv. Bonnie Best) were grown in a pathogen-infested, soilless rockwool system in the greenhouse and were fertilized with a nutrient solution that was amended with macro- and microelements at various rates. Disease was evaluated after 2 weeks using an index of 0 to 4, and plant fresh weight was measured. Regression analysis indicated that disease severity was significantly increased by ammonium-nitrogen [NH4Cl, (NH4)6Mo7O24, and (NH4)2SO4], NaH2PO4·H2O, Fe-EDDHA, MnSO4, MoO3, and ZnSO4·7H2O. Disease severity was reduced by nitrate-nitrogen [Ca(NO3)2·4H2O] and CuSO4·H2O. Low rates of NH4NO3 (39 to 79 mg·L-1 N) reduced disease, but rates above 100 mg·L-1 N increased it. Disease was not affected by MgSO4·7H2O. In all cases, plant growth was inversely related to disease severity. Mineral fertilizers had no effect on nutrient solution pH. This information sheds new light on environmental factors that influence plant-pathogen interactions, and may be applied to develop a management strategy for Fusarium crown and root rot based on host nutrition.
Changhoo Chun, Machiko Tominaga, and Toyoki Kozai
We recently showed that spinach (Spinacia oleracea L.) transplants produced under a short photoperiod and low air temperature were characterized by a delay of bolting and short flower-stalk length at harvest (Chun et al., 2000a). The present study was conducted to determine whether these changes are caused by the short photoperiod itself or by the lower integrated photosynthetic photon flux (IPPF). Shoot and root dry weights of transplants increased significantly with increasing IPPF, but were not affected by a change in the photoperiod. However, the floral development indices of transplants were significantly greater under a 16-than under a 10- or 13-hours/day photoperiod, but were not affected by a change in IPPF. The percentage of bolted plants 3 days after transplanting (DAT) increased significantly with increasing photoperiod (from 0% at 10 hours/day to more than 85% at 16 hours/day). Flower-stalk length increased with increasing photoperiod (e.g., at 14 DAT, from 15 mm at the shorter photoperiods to 80 mm at 16 hours/day), but was not affected by a change in IPPF. These results show that the delay of bolting that occurs when the photoperiod is reduced during transplant production is due to the delay of floral development and not to retarded vegetative growth as a result of reduced IPPF.
Wlodzimierz Bres and Leslie A. Weston
`Buttercrunch', `Grand Rapids', and `Summer Bibb' lettuce (Lactuca sativa L.) seedlings were grown with the nutrient film technique (NIT). The influence of two K concentrations (150 and 225 mg·liter-1) and four solution pH levels (5.0, 5.5, 6.0, and 6.5) on lettuce tipburn was investigated in four experiments. Additionally, the influence of pH on foliar nutrient concentration was examined. Even though tipburn was observed in `Buttercrunch' and `Summer Bibb' lettuce, neither K nor pH level consistently affected tipburn incidence. No tipburn was observed in `Grand Rapids'. Solution pH generally did not affect concentration of total N and NO3-N in lettuce tissue. Increasing the pH increased K concentration and resulted in increased proportions of K compared to Mg or Ca. Although the influence of solution pH on P, Ca, and Mg concentration was significant, nutrient accumulation differences were not reflected in lettuce fresh-weight differences. The influence of K solution concentration and pH on lettuce yield was not significant. Tipburn incidence in NIT-produced lettuce appears to be primarily affected by environmental conditions maintained during greenhouse growth.
Dean A. Kopsell, Kimberly J. Whitlock, Carl E. Sams, and David E. Kopsell
Purslane (Portulaca oleracea) is a succulent weedy annual in much of the United States. In other parts of the world, purslane is grown as a specialty crop, valued for its nutritional quality. As a leafy crop, purslane contributes carotenoid phytochemicals in the typical Mediterranean diet. Nitrogen (N) influences plant growth and alters pigment composition and accumulation. However, little is known about the impact N fertility may have on pigment concentrations in purslane shoot tissues. The objective of this study was to evaluate the influence of N fertility levels on biomass and concentrations of nutritionally important carotenoid and chlorophyll pigments in purslane. Green Leaf and Golden Leaf purslane cultivars were grown in nutrient solution culture under N concentrations of 13, 26, 52, or 105 mg·L−1. Plants were harvested at 45 days after planting (DAP), and measured for concentrations of shoot pigments using high-performance liquid chromatography (HPLC) methodology. There was no influence of N treatment concentration on purslane shoot tissue fresh weight (FW) accumulation. Nitrogen treatment significantly influenced shoot tissue β-carotene (BC), lutein (LUT), neoxanthin (NEO), total carotenoids, chlorophyll a, chlorophyll b, total chlorophyll, and the chlorophyll a to b ratio in purslane shoot tissues. Concentrations of LUT, NEO, violaxanthin (VIO), chlorophyll b, total xanthophyll cycle pigments, and the chlorophyll a to b ratio differed between the purslane cultivars. Increases in N concentrations acted to increase concentrations of nutritionally important shoot tissue carotenoid pigments in only the Green Leaf purslane cultivar. Therefore, N fertility management and cultivar selection should be considered when producing purslane as a nutritious specialty vegetable crop.