the injected samples at a certain wavelength (470 nm) in the visible spectrum. N content was determined according to Jackson (1958 ). Measurement of MDA contents. Lipid peroxidation was determined by measuring the total amount of malondialdehyde (MDA
activity and lipid peroxidation. Fully expanded leaf blades were excised and then wrapped in foil paper, immersed in liquid nitrogen, and stored at −20 °C until measurement was determined. Determination of enzyme activity was carried out using the method
stress and other abiotic stresses are associated with the production of reactive oxygen species (ROS), such as superoxide anion, hydrogen peroxide (H 2 O 2 ), and hydroxyl radical, that cause lipid peroxidation in plasma, mitochondria, peroxisome, and
associated to NADPH oxidation at 25 °C, was determined at 340 nm (ε = 6.22 m m −1 ·cm −1 ). The assay buffer was 100 m m Tris-HCl (pH 7.8), 2 m m EDTA, 0.5 m m glutathione in the oxidized form (GSSG). Estimation of lipid peroxidation. Five grams of
chickling-pea ( Lathyrus sativus ) seedlings as measured by the degree of lipid peroxidation ( Xiong et al., 2006 ). Glycine betaine has also been reported to scavenge for ROS ( Smirnoff and Cumbes, 1989 ) and protect plants from salinity-induced oxidative
Chen, 2007 ). Senescence of plant tissues is generally accompanied by respiration increases; breakdown of carbohydrates, proteins, lipids, and nucleic acids ( Singh et al., 2000 ); higher production of reactive oxygen species (ROS) ( Del Rio et al
Cauliflowers (Brassica oleracea) were irradiated at 0, 2, and 4 kGy and stored 8 days at 13°C. Development of yellow color and browning of the in florescence, increase in membrane electrolyte leakage and reduction of protein recovery in microsomal membranes were observed over the storage period. Changes in membrane free fatty acids, lipid phosphorus content, peroxydation level, and fatty acid composition of polar lipids also occurred. These results indicate an important modification of cellular membranes. The direct effect of gamma rays on membrane lipids via free radical production and subsequent destabilization of the lipid bilayer during storage could be responsable for earlier onset of senescence.
gain a better understanding of the regulation of lipid metabolism. In this study, the FA content and composition in developing seeds were analyzed and a time-series analysis of transcriptomic data was performed to reveal DEGs in six phases of A
Cucurbitaceae seeds ( Ramakrishna and Amritphale, 2005 ; Yim and Bradford, 1998 ). This envelope is reported to consist of a single cell layer of endosperm whose outer surface is covered by lipid and callose layers ( Yim and Bradford, 1998 ). The PE envelope of
), hydroxyl radical (OH*), and hydrogen peroxide (H 2 O 2 ), which can attack lipids, proteins, carbohydrates, and nucleic acids ( Smirnoff, 1993 ). To minimize and eliminate oxidative damage, plants have evolved an antioxidant defense system comprising