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described by Nour et al. (2010) . Chromatographic separation was performed with an HPLC system (1260 Series; Agilent Technologies, Santa Clara, CA), using 50 m m potassium dihydrogen orthophosphate buffer, pH 2.8, as a mobile phase (flow rate of 0.7 mL

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followed the procedure of Anderson et al. (1992) . Mung bean leaves (1 g fresh weight) were homogenized in 3 mL of ice-cold acidic extraction buffer [6% (w/v) meta-phosphoric acid (pH 2.8), containing 1 m m EDTA], using a Polytron homogenizer (model PT

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maintained at 30 °C using a thermostat column compartment. All separations were achieved using gradient mobile phase of 1) reverse osmosis water adjusted to pH 2.5 with trifluoroacetic acid and 2) acetonitrile. The gradient held the following percentages: 15

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aqueous solution were used as the mobile phase, the flow rate was 0.4 mL⋅min −1 , the detection wavelength was 255 nm, and the injection volume was 10 μL. A 1-g sample was ground with 10 mL of isopropanol hydrochloric acid buffer (pH = 2.2; isopropanol

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, pH 2.4, flow rate: 1.0 mL·min –1 , column temperature: 30 °C, detection wavelength 210 nm. Each sample was injected three times, and the corresponding organic acid or sugar content was calculated using the obtained standard curve. The average value

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, which can have secondary effects on potassium (K) uptake in plants. The objectives of this review are to 1) describe the impact of DL rate on substrate pH, 2) discuss the primary and secondary effects of DL on nutrient availability, and 3) summarize the

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cool to room temperature, and the solution was filtered through wet filter paper and collected in a 50-mL volumetric flask. The residue was mixed with 20 mL of citrate buffer (pH 2.2) and the amino acid profile was determined using an automatic amino

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a BEH C18 column (Aquity, Waters Co). Each well was injected in triplicate with 5 μL of sample volume. The mobile phases consisted of 5 m m potassium phosphate monobasic (KH 2 PO 4 ), pH 2.65, with 0.1% of formic acid (mobile phase A) and methanol

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pH using a pH meter (Model 215; Denver Instrument, Bohemia, NY). We extracted P, K, Ca, Mg, Na, and S using the Mehlich III extractant (a dilute acid-fluoride-EDTA solution of pH 2.5 that consists of 0.2 N CH 3 -COOH, 0.25 N NH 4 NO 3 , 0.015 N NH 4 F

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temperature ( WSU AgWeatherNet, 2019 ). During the growing season, the average RH is 80%, while total precipitation is 39 mm per month. The soil is Skagit silt loam, a fine-silty mixed nonacid mesic Typic Fluvaquents, with 6.5 pH, 2.7% organic matter, and is

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