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Ellen T. Paparozzi, Joshua R. Widhalm, and M. Elizabeth Conley

Common swedish ivy plants when exposed to nitrogen (N) stress display typical nitrogen deficiency symptoms such as reddening of stems and petioles and yellowing of leaves. When N levels are restored, leaves of swedish ivy plants will re-green without leaf loss. An experiment was conducted to determine how proteins change when leaves were re-greened after N deficiency. Cuttings of Plectranthus australis were rooted under mist and allowed to yellow. Plants were then potted up and fertilized with one of two treatments: complete nutrients with N at 150 ppm or complete nutrients with 0.8 ppm N. The experimental design was a randomized complete-block design with six blocks. Each block had the two N treatments and six plants per treatment. After 3–4 weeks, all plants in the 150-ppm N treatment had re-greened and leaf samples for protein analysis were taken. Plants in four of the six blocks were then switched to the other treatment. After leaves had re-greened once again, leaf samples were taken and the experiment was terminated. Two-dimensional polyacrylamide gel electrophoresis was used to compare the treatments. No obvious differences in protein absence or presence were noted. However, Rubisco appeared to be differentially expressed between the two treatments. 2-D gel analysis with subsequent Western blots showed that for most of the leaf samples, the large subunit of Rubisco (56kD) was quantitatively about 1.3 times more concentrated in the N-deficient plants and possibly modified. The small subunit (12kD) was not reliably detectable. Additional protein results for repeated leaf re-greening and the role Rubsico may play in leaf re-greening will be discussed.

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James E. Altland, James C. Locke, Wendy L. Zellner, and Jennifer K. Boldt

zonal and ivy geranium ( Pelargonium × hortorum L.H. Bailey and P. peltatum (L.) L’Hér. ex Aiton, respectively) ( Andrews and Hammer, 2006 ), petunia ( Petunia × hybrida (Sweet) D. Don ex W. H. Baxter), impatiens ( Impatiens walleriana Hook

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James E. Altland and Charles Krause

clippings. Kresten Jensen et al. (2001) reported that English ivy ( Hedera helix L.) grew well in composted miscanthus ( Miscanthus ogiformis ) substrates, although dry matter accumulation was greater in peat-based substrates. Dresboll and Thorup

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Chase Jones-Baumgardt, David Llewellyn, Qinglu Ying, and Youbin Zheng

arrays (LX601C, Heliospectra AB, Gothenburg, Sweden) were used to provide six PPFD treatments: 100, 200, 300, 400, 500, and 600 μmol·m −2 ·s −1 ( Table 1 ), measured at the level of the top of the substrate. These fixtures have four separately

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Ellen T. Paparozzi, Jazbaat K. Chahal, Petre Dobrev, Elizabeth A. Claassen, Walter W. Stroup, and Radomira Vankova

. Recently, we described that P. parviflorus (swedish ivy) responds to N deficiency by yellowing of leaves and cessation of growth to such an extent that the plant appears dormant ( Korus et al., 2010 ). However, no leaves are lost. On reintroduction of N

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Chase Jones-Baumgardt, David Llewellyn, and Youbin Zheng

setup. Ten LED fixtures (LX502G; Heliospectra AB, Gothenburg, Sweden) were hung in pairs 1.4 m above the benches near each of the four corners and in the geometric center of the four-bench growing area. The Euclidian coordinates (in meters; from the