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Aoxue Wang, Fanjuan Meng, Xiangyang Xu, Yong Wang, and Jingfu Li

intensive and time-consuming. The development of molecular markers provides the possibility of rapid screening of resistant germplasm for breeding. Moreover, molecular markers tightly linked to resistant genes also provide the basis for isolating and cloning

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V. Meglic and R.T. Chetelat

57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management

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Charles J. Simon and Richard C. Johnson

57 WORKSHOP 8 Use of Molecular Markers in Germplasm Management

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R.A. Teutonico, T.C. Osborn, and J.P. Palta

30 POSTER SESSION 4 (Abstr. 460-484) Breeding/Genetics/Molecular Markers

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J.I. Hormaza, L. Dollo, and V.S. Polito

115 ORAL SESSION (Abstr. 227-233) Tree Fruits and Nuts: Genetics/heeding/Molecular Markers

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Tong Geon Lee, Reza Shekasteband, Naama Menda, Lukas A. Mueller, and Samuel F. Hutton

this trait could be very helpful for introducing and selecting the jointless trait in tomato germplasm, especially for fresh-market backgrounds which are predominantly jointed. The objective of this project was to develop molecular markers linked to the

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Salih Kafkas, Yıldız Doğan, Ali Sabır, Ali Turan, and Hasbi Seker

; Erdogan and Mehlenbacher, 1997 ; Yao and Mehlenbacher, 2000 ; Koksal, 2002 ). In recent years, researchers around the globe have investigated genetic relationships among hazelnut cultivars using several types of molecular markers, including randomly

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Nader R. Abdelsalam, Rehab M. Awad, Hayssam M. Ali, Mohamed Z.M. Salem, Kamal F. Abdellatif, and Mohamed S. Elshikh

., 2013 ). Although they are affected by environmental conditions, morphology and agronomic characteristics are helpful tools for the survival of plant species diversity. A wide range of molecular markers are used to estimate genetic polymorphism, and

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Peggy Ozias-Akins, Edward L. Lubbers, and Wayne W. Hannna

Apomixis is asexual reproduction through seed. Apomixis in the genus Pennisetum is of the gametophytic (aposporous) type. Genes for apomixis have been transferred from a wild apomictic species (P. squamulatum) to pearl millet (P. glaucum) by conventional breeding to produce an obligately apomictic backcross 3 (BC3) plant (Dujardin and Hanna, 1989, J. Genet. Breed. 43:145). Molecular markers based on restriction fragment length polymorphisms and random amplified polymorphic DNAs were identified in BC3 that were shared only with the apomictic parent. Segregation of these informative markers in a BC4 population indicated that three linkage groups from P. squamulatum were present in BC3 and that minimal recombination between these alien chromosomes and those of the recurrent parent occurred. Transmission of only one of the linkage groups was required for transfer of apomixis. Recombination is essential for genetic mapping, thus we are beginning to map the informative molecular markers in an F, interspecific cross between pearl millet and P. squamulatum, a population that segregates for apomictic and sexual reproduction.

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Ryutaro Tao, Tsuyoshi Habu, Hisayo Yamane, Akira Sugiura, and Kazuya Iwamoto

Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no pollinizer is required. Self-incompatibility is gametophytic, as in other Prunus species. We searched for molecular markers to identify self-compatible cultivars based on the information about S-ribonucleases (S-RNases) of other Prunus species. Total DNA isolated from five self-incompatible and six self-compatible cultivars were PCR-amplified by oligonucleotide primers designed from conserved regions of Prunus S-RNases. Self-compatible cultivars exhibited a common band of ≈1.5 kbp. Self-compatible cultivars also showed a common band of ≈12.1 kbp when genomic DNA digested with HindIII was probed with the cDNA encoding S 2-RNase of sweet cherry (Prunus avium L.). These results suggest that self-compatible cultivars of Japanese apricot have a common S-RNase allele that can be used as a molecular marker for self-compatibility.