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Cindy B.S. Tong, Hsueh-Yuan Chang, James J. Luby, David Bedford, Benham E.L. Lockhart, Roy G. Kiambi, and Dimitre Mollov

et al., 2011 ), and Pepino mosaic virus ( Moerkens et al., 2015 ). ACLSV, Apple stem grooving virus (ASGV), and ASPV are widely distributed and commonly occurring in apples. All three are single-stranded RNA viruses, typically latent (symptomless

Open access

H. Brent Pemberton, Kevin Ong, Mark Windham, Jennifer Olson, and David H. Byrne

Rose rosette disease is incited by a negative-sense RNA virus (genus Emaravirus ), which is vectored by a wind-dispersed eriophyid mite ( P. fructiphilus ) ( Di Bello et al., 2015a ; Laney et al., 2011 ). Symptoms on roses include witches broom

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K.S. Ling, C.A. Clark, C. Kokkinos, J. R. Bohac, S.S. Hurtt, R. L. Jarret, and A. G. Gillaspie

Sweet potato virus disease (SPVD) is the most devastating virus disease on sweetpotato [Ipomoea batatas (L.) Lam] world wide, especially in East Africa. However, weather it is present in the U.S. is unknown. SPVD is caused by co-infection of sweetpotato feathery mottle virus (SPFMV) and sweetpotato chlorotic stunt virus (SPCSV). Presence of two other potyviruses, sweetpotato virus G (SPVG) and Ipomoea vein mosaic virus (IVMV) has also been confirmed in the U.S. Sweet potato leaf curl virus (SPLCV), a whitefly (Bemisia tabaci) transmitted Begomovirus, also has the potential to spread to commercial sweetpotato fields and poses a great threat to the sweetpotato industry. The U.S. collection of sweetpotato germplasm contains about 700 genotypes or breeding lines introduced from over 20 different countries. Newly introduced sweetpotato germplasm from foreign sources are routinely screened for major viruses with serology and graft-transmission onto indicator plants (Ipomoea setosa). However, a large portion of this collection including heirloom cultivars or old breeding materials has not been systemically screened for these major sweetpotato viruses. In this study, a total of 69 so-called heirloom sweetpotato PI accessions were evaluated for their virus status. We used Real-time PCR to detect five sweetpotato viruses, including four RNA viruses (SPCSV, SPFMV, SPVG, and IVMV) and one DNA virus (SPLCV). A multiplex Real-time RT-PCR system was developed to detect three RNA viruses (SPFMV, SPVG, and IVMV). Preliminary data indicated that about 15% of these heirloom sweetpotato germplasm carried at least one of these viruses tested. Details on virus infection status will be presented.

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Binoy Babu, Gary Knox, Mathews L. Paret, and Francisco M. Ochoa-Corona

single-stranded negative-sense RNA virus ( Laney et al., 2011 ). The virus is transmitted by the eriophyid mite species Phyllocoptes fructiphilus ( Amrine et al., 1988 ; Laney et al., 2011 ) and by grafting ( Amrine et al., 1988 ). The mites do not fly

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Sahar Eid, Keri L. Druffel, Dayle E. Saar, and Hanu R. Pappu

Register of Dahlia Names, 1969 et seq. ). There are more than a dozen viruses that infect dahlia ( Albouy, 1995 ). All of these viruses are RNA viruses except one DNA virus, Dahlia mosaic caulimovirus (DMV). DMV is widely prevalent in cultivated dahlias

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Karen R. Harris, Kai-Shu Ling, W. Patrick Wechter, and Amnon Levi

confers resistance to an uncapped and non-polyadenylated RNA virus in melon Plant J. 48 452 462 Prins, M. Laimer, M. Noris, E. Schubert, J. Wasseneggar, M. Tepfer, M. 2008 Strategies

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Michael J. Havey and Yul-Kyun Ahn

(accessions JZ773807 to JZ774940). Six hundred and twenty (26%) of the random reads were highly similar to garlic virus D, an RNA virus that commonly infects garlic ( Wylie et al., 2014 ) and known to occur in the northwestern United States ( Gieck et al

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Cristina Zambrana-Echevarría, Lorriane De Jesús-Kim, Rocio Márquez-Karry, Dimuth Siritunga, and David Jenkins

selection and recombination are factors that also influence the genetic variability in viral populations ( Fernández-Rodríguez et al., 2008 ) and their role in shaping PR-PRSV’s population cannot be discarded. Furthermore, RNA viruses are most susceptible to

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Xiaohong Wang, Bishun Ye, Xiangpeng Kang, Ting Zhou, and Tongfei Lai

viral genome explicitly ( Senthil-Kumar and Mysore, 2011 ). PVX, a single-strand RNA virus, is a member of the potexvirus group ( Lico et al., 2006 ). The PVX-mediated VIGS has been used successfully for gene function analysis in tomato fruit ( Chen et

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Zongrang Liu, Ralph Scorza, Jean-Michel Hily, Simon W. Scott, and Delano James

silencing mechanism ( Baulcombe, 1996b ). During the silencing process, the dsRNAs (formed from aberrant transcripts in transgenic plants, RNA transcripts that produce hairpins through inverted repeats, or RNA virus replicate intermediates) are degraded into