Diagnosis and recommendation integrated system (DRIS) leaf blade and petiole optimum nutrient ranges were developed through tissue sampling in 53 commercial strawberry (Fragaria ×ananassa) fields in the coastal valleys of central California in 2010 and 2011. All fields were in an annual production system using the day-neutral cultivar Albion. Leaf blades and petioles were sampled five times from early flowering through the fruit harvest period. Data on soil nutrient availability and grower fertilization practices were also collected. DRIS analysis was used to develop nutrient optimum ranges based on nutrient concentrations observed in nutritionally balanced, high-yield fields. Blade nitrogen (N), phosphorus (P), and potassium (K) concentrations declined from the vegetative stage until the main harvest period, and stabilized thereafter. Blade calcium (Ca), boron (B), and iron (Fe) increased over time while magnesium (Mg), sulfur (S), manganese (Mn), zinc (Zn), and copper (Cu) decreased. The blade N optimum range was lower than previously published sufficiency ranges during the fruit harvest period, and the Zn optimum range was lower throughout the season. Other nutrients were in general agreement with previously established sufficiency ranges with the exception of Ca, Mn, and Fe, which were higher. Petiole nitrate-nitrogen (NO3-N) was highly variable among high-yield fields, was not correlated with soil NO3-N at any growth stage, and was therefore of limited value as an indicator of crop N status. Comparison of soil nutrient availability with grower fertilization practices suggested that significant improvement in fertilizer management was possible.