anthuriums for multiple traits including pest tolerance ( Kamemoto and Kuehnle, 1996 ; T. Amore, unpublished results), genetic transformation of important but highly susceptible cultivars could provide a faster solution for growers. Previously, transformation
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Maureen M.M. Fitch, Terryl C.W. Leong, Xiaoling He, Heather R.K. McCafferty, Yun J. Zhu, Paul H. Moore, Dennis Gonsalves, Herb S. Aldwinckle, and Howard J. Atkinson
Wenhao Dai, Yuanjie Su, Hongxia Wang, and Ceilo Castillo
and Arce-Johnson, 2005 ). However, transformation of Buddleia species has not been documented to date. The objective of this study was to develop a gene transformation protocol for further improvement of Buddleia species. Materials and Methods
Chunxian Chen, Qifa Zheng, Xu Xiang, Jaya R. Soneji, Shu Huang, Young A Choi, Madhugiri Nageswara Rao, and Fred G. Gmitter Jr.
Different serials of binary vectors such as pCAMBIA, pGA, pBIN, pGreen with regular insert capability, and BiBACs, pYLTACs with large insert capability have been reported for Agrobacterium -mediated transformation (AMT) of various plant species
Zhanyuan Zhang, Dermot P. Coyne, and Amitava Mitra
1 Dept. of Horticulture; to whom reprint requests should be addressed. Present address: E325, Plant Transformation Facility, George W. Beadle Center, Univ. of Nebraska-Lincoln, Lincoln, NE 68588-0665. ( hort045@unlvm.unl.edu ). 2 Dept. of
Julie A. Russell, Mihir K. Roy, and John C. Sanford
179 ORAL SESSION (Abstr. 731-735) Cell and Tissue Culture: Transformation
Wenhao Dai, Victoria Magnusson, and Chris Johnson
species ( Poupin and Arce-Johnson, 2005 ). In the genus Prunus , transformation of several species have been documented, including peach ( Hammerschlag and Smigocki, 1998 ; Pérez-Clemente et al., 2004 ; Scorza et al., 1995a ), plum ( Scorza et al., 1995
Kelly A. Zarka, Ria Greyling, Inge Gazendam, Dean Olefse, Kimberly Felcher, Gurling Bothma, Johan Brink, Hector Quemada, and David S. Douches
tumefaciens -mediated transformation ( De Buck et al., 2000 ; Oltmanns et al., 2009 ). Therefore, it is necessary to determine if a GM plant carries backbone sequences and to select those that do not. Once a transgenic event has been selected, one must
Cong Li, Lie-Bao Han, and Xunzhong Zhang
of the ERF subfamily. The tobacco plant is an important cash crop in China and other regions and has also been widely used for genetic transformation. The objectives of this study were to introduce OjERF into tobacco plants and investigate drought
Mary C. Stevens, Rui Yang, and Joshua H. Freeman
important to assess the deposition of nitrogen by EDN. One area of concern regarding soil fumigants is their impact on soil microbes, some of which are responsible for nitrogen transformation ( Ibekwe et al., 2001 ). Generally, a significant increase of
Kathryn Kamo and Bong Hee Han
the transformation of lilies. Agrobacterium -mediated transformation of Lilium longiflorum and Oriental lilies has been demonstrated ( Hoshi et al., 2004 ; Mercuri et al., 2003 ; Ogaki et al., 2008 ). Biolistic-mediated transformation has been