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Kenneth W. Mudge and Chin-Chang Chu

In vitro asymbiotic seed germination, subculture, and outplanting of orchids is presented as a laboratory exercise suitable for students of plant propagation or tissue culture. Dendrobium antennatum (Lindley), Phalaenopsis (Blume) white hybrid, or both, are used in this exercise because they flower predictably in the greenhouse, are reliable for seed production, and germinate and grow rapidly in vitro. The exercises can be used to instruct students in the skills involved in orchid seed sterilization, sowing, and culture, as well as instruct students in the unique features of orchid reproductive biology and symbiosis. A schedule is suggested for stock plant flower pollination, capsule harvest, seed sowing, and seedling subculture so that the necessary plant material is available for students to sow, subculture, and outplant seedlings during a single laboratory session.

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Marco A. Palma, Yu-Jen Chen, Charles Hall, David Bessler, and David Leatham

Orchids are the fastest-growing group of potted flowering plants in the United States in terms of sales, ranked second behind poinsettia ( Euphorbia pulcherrima ) among all potted flowering plants. Southern and western states are the main producing

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Yihui Cui, Peng Zhao, Hongqiang An, Nan Lv, Zifeng Zhang, Wei Pei, and Wanjun Wang

Dendrobium candidum Wall ex Lindl., a monocotyledon belonging to the family Orchidaceae, is distributed in countries of South and Southeast Asia. As an endangered species, mass propagation of this orchid has been actively studied ( Shiau et al

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Wagner A. Vendrame, Aaron J. Palmateer, Ania Pinares, Kimberly A. Moore, and Lawrence E. Datnoff

ornamental plants, orchid production has increased over the past 10 years as a result of their popularity, the rapid expansion of the market, and the interest of growers and customers for new and improved hybrids. By 2004, orchids ranked second among potted

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Matthew G. Blanchard and Erik S. Runkle

Orchids are the second most valuable potted flowering plant in the United States with a reported wholesale value of $123 million in 2006, an increase of 162% in the past decade ( U.S. Department of Agriculture, 2007 ). In 2006, 14.7 million potted

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Xiangli Ma, Min Tang, Yufen Bi, and Junbo Yang

101°E. C. tortisepalum is famous for its graceful shape, varied flowers, and beautiful color, which gives it a high ornamental and economic value, and it has become one of the most sought-after orchid species in Yunnan Province ( Du Puy and Cribb

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Yin-Tung Wang

1 Associate Professor of Floriculture. Orchid plants were donated by Taiwan Sugar Corp., Taipei, Taiwan, Republic of China. Lori L. Gregg's assistance is greatly appreciated. The cost of publishing this paper was

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Chairani Siregar

Orchids are members of the Orchidaceae, the largest family among flowering plants, which includes 25,000 species from 850 genera. It is estimated that 2500 to 3000 orchid species grow in the forests of Borneo ( Irawati, 2002 ). In their natural

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C.S. Hew, L.Y. Lim, and C.M. Low

The uptake of nitrate and ammonium by a terrestial (Bromheadia finlaysonia) and an epiphytic (Dendrobium hybrid) orchid in solution culture has been studied. The rates of nitrate and ammonium were relatively linear, with higher rate of uptake for ammonium. The rates of nitrate uptake in terrestial and epiphytic orchids were 0.4 and 0.9 μmole gm fw-1 hr-1 respectively and they were considerably lower than those of most major crops. SEM studies show that the velamen of Bromheadia was 2 cells thick whereas that of Dendrobium was 8-10 cells thick. It is unlikely that the velamen is the major factor in restricting influx of nitrate or ammonium. Nitrate reductase (NR) and glutamine synthetase (GS) were present in roots and leaves of both orchids. NR was high in roots but low in leaves. The reverse was for GS. The activities of NR and GS was low but high enough to account for the rate of nitrate or ammonium uptake. It appears that the movement of ions across the transfer junction at the exodermis plays a major regulatory role in ion uptake by orchid root.

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Kaylee A. South, Paul A. Thomas, Marc W. van Iersel, Cindy Young, and Michelle L. Jones

Orchids are currently the top-selling potted flowering plant in the United States because of their long flower life and compact growth ( Banks, 2005 ; Fitch, 2004 ; USDA, 2016 ). While the popularity of many potted plants is declining, the number