cultivars ( Li et al., 2002 ) treated with TDZ.
Understanding morphological responses during in vitro culture is critical for understanding regeneration pathways. Recent reports illustrate the importance of histological evaluations of in vitro culture
Johnson, 1976 ). It is possible that the pressure-infiltration procedure may also contribute to the injury symptoms that appear after treatment and storage. Therefore, the objective of this investigation was to histologically examine the effects of
research was supported by Federal Formula Funds, Regional Project NE103. We thank Mary Jean Welser for the histological preparations of material in this study. The cost of publishing this paper was defrayed in part by the payment of page charges. Under
this study, we histologically demonstrate the factors of seedless expression in ‘Mukaku Kishu’, ‘Southern Yellow’, and the hybrid seedlings of ‘Southern Yellow’ × pummelo.
Materials and Methods
‘Hira Kishu’, ‘Mukaku Kishu’, ‘Tanikawa Buntan
. Sta.). Histological variables (union line, callus proliferation, and deposit intensity) were used to discriminate the compatible from the incompatible combinations [adapted from Ermel et al. (1995) ].
Results and Discussion
There was an
biology, the present work aims to describe the morphology and anatomy of bisexual and functionally male flower types in pomegranate. Morphological and histological evaluations of hermaphroditic and male flowers were conducted using light microscopy (LM
The histology and morphology of developing asparagus Asparagus officinalis L.) somatic embryos arising in callus cultures were examined and contrasted with that documented for zygotic embryos. Histological sections of lateral bud-derived callus cultured for 2 weeks on embryo induction medium consisting of Murashige and Skoog salts and vitamins (MS) with 1.5 mg NAA/liter and 0.1 mg kinetin/liter indicated the formation of distinct groups of embryogenic cells. At 4 weeks, the callus was comprised of embryos in the early and late globular stages and a few bipolar embryos. Within 2 weeks on embryo development medium consisting of MS with 0.05 mg NAA/liter and 0.1 mg kinetin/liter, the globular embryos developed a bipolar shape having an expanded upper region that formed the cotyledon and a smaller region that formed the radicle. Within 4 to 6 weeks on this latter medium, each mature bipolar embryo was opaque and had a large cotyledon, a distinct shoot apex at the cotyledon-hypocotyl junction, and vascular connections between the radicle, shoot apex, and cotyledon. Many mature somatic embryos resembled the asparagus zygotic embryos in having a crescent shape, whereas others had a short but wide cotyledon. Both somatic embryo types converted to plantlets at equal rates. Chemical names used: N- (2-furanylmethyl)-1 H -purin-6-amine (kinetin); 1-naphthaleneacetic acid (NAA).
under a microscope and to identify histological differences between vegetative and reproductive primordia.
The objectives of this study were to investigate the effect of nutrient termination date and reapplication time on growth and flowering, to study
water loss, high starch accumulation, and a low soluble sugar content ( Chaplin et al., 1991 ; Chhatpar et al., 1971 ; Gupta and Jain, 2014 ; Medlicott et al., 1990 ; Mohammed and Brecht, 2002 ). Histological observation revealed that starch granules
used for cell histology. Cell histology. For each harvested fruit, four pericarp samples were fixed overnight at room temperature in a 1 acetic acid:2 formaldehyde:5 ethanol solution. Sections of 3 μm thick were stained using toluidine blue and