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Kelly M. Oates, Thomas G. Ranney, Darren H. Touchell, and Zenaida Viloria

(2 n = 3 x ). Male and female fertility was significantly reduced in ‘Chastity’ as assessed by pollen staining, pollen germination, and controlled crosses ( Table 2 ). Pollen staining was performed on fresh pollen from each accession. Pollen was

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Virginia Pinillos and Julián Cuevas

-pollination deficits that can be solved by regrafting some trees with suitable pollenizers ( Lavee and Datt, 1978 ) or by mechanical application of compatible pollen ( Sibbett et al., 1992 ). Some authors have proposed 30 to 40 m as the maximum distance from

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Dongmei Wei, Huimin Xu, and Ruili Li

mature pollen. ( D ) Anthers during the seven stages. ( E ) Transverse section of an anther at the sporogenous cell stage. Neutral lipids (stained black) are evident in the center of sporogenous cells, and some starch (stained red) is visible in tapetal

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Mohammad Sadat Hosseini Grouh, Kourosh Vahdati, Mahmoud Lotfi, Darab Hassani, and Nejat Pirvali Biranvand

of irradiated pollen as mentor pollen to induce self-fertilization of two self-incompatible upper amazon cacao clones Euphytica 51 219 225 Bassam, B.J. Caetano-Anolles, G. Gresshoff, P.M. 1991 Fast and sensitive silver staining of DNA in

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Thomas M. Kon, Melanie A. Schupp, Hans E. Winzeler, and James R. Schupp

strategy, the effects of TS temperature and treatment duration on pollen tube growth in vivo and visible spur leaf injury were evaluated. Materials and Methods Trials were conducted in 2014 and 2015 at the Pennsylvania State University’s Fruit Research and

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Alisha L. Ruple, John R. Clark, and M. Elena Garcia

the slide. One to two drops of aniline blue stain were placed on top of the pollen, and samples were covered and allowed to stain at least 10 min ( Kearns and Inouye, 1993 ). Samples were viewed under a light microscope and 500 pollen grains were

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Weiping Zhong, Zhoujun Zhu, Fen Ouyang, Qi Qiu, Xiaoming Fan, and Deyi Yuan

reproductive organ of angiosperms. The development of anthers, the parts of the stamen where pollen is produced, is a delicate and complex process that includes the formation of the anther wall tissue (with a protective function) and pollen (with a reproductive

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Alberto Sánchez-Estrada and Julián Cuevas

orchard. CP treatments were achieved by applying either ‘Barouni’ fresh pollen or purchased ‘Sevillano’ pollen to the stigmas of ‘Manzanillo’ flowers using a fine paintbrush. Pollen viability was ascertained before use by the fluorochromatic staining test

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Guanxing Hu, Chao Gao, Xiaoming Fan, Wenfang Gong, and Deyi Yuan

Xenia is defined as the effect of foreign pollen on the development of fruit tissue. It involves the interaction between a nuclear gene from the male gamete and either two polar nuclear genes from the endosperm or one nuclear gene from the egg

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Guirong Li, Ran Quan, Chaohui Yan, Xiaojin Hou, and Huiling Hu

deionized water. Fresh solutions were prepared for each experiment. Pollen was stained in a working solution for 30 min before observation. Counts were made at random in three fields, and pollen was photographed under an inverted fluorescence microscope [10