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Wenjing Guan, Xin Zhao, Richard Hassell, and Judy Thies

melon necrotic spot virus in cucurbits was a significant advantage over soil fumigation with methyl bromide, which does not control this viral disease ( Cohen et al., 2007 ). Meanwhile, tomato yellow leaf curl virus , tomato spotted wilt virus , and

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Jollanda Effendy, Don R. La Bonte, and Niranjan Baisakh

thickness (1.2 mm) at 0 (control), 2, 4, 8, and 12 h after skinning and the peels were immediately frozen in liquid nitrogen and stored at –80 °C for RNA extraction. Three independent roots were used for each time point as replicates. RNA Isolation, cDNA

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John R. Stommel, Gordon J. Lightbourn, Brenda S. Winkel, and Robert J. Griesbach

area of absorption per gram of fresh weight of three replicates. Gene expression. Flowers, immature fruit, and leaves were harvested from actively growing plants, frozen in liquid nitrogen, and stored at −80 °C. Total RNA was isolated from ≈100

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Hisanao Suzue, Munetaka Hosokawa, and Susumu Yazawa

tissue ( Laimer, 2003 ). Several pathogens such as viruses and viroids can exist in the youngest leaf primordium ( Knapp et al., 1995 ; Rodio et al., 2007 ), making regeneration from very small pieces of tissue containing SAM very important for

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James J. Polashock, Rajeev Arora, Yanhui Peng, Dhananjay Naik, and Lisa J. Rowland

) of the relatively cold-hardy highbush cultivar Bluecrop and the relatively cold-sensitive rabbiteye cultivar Tifblue. RNA was extracted from floral buds of ‘Bluecrop’ and ‘Tifblue’ field plants that had accumulated 0 (collected 29 Sept. 2003), 67 (20

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Concetta Licciardello, Biagio Torrisi, Maria Allegra, Fabiola Sciacca, Giancarlo Roccuzzo, Francesco Intrigliolo, Giuseppe Reforgiato Recupero, Paola Tononi, Massimo Delledonne, and Vera Muccilli

). In addition, although the sour orange is the most widely distributed and common rootstock in the Mediterranean area and it has adapted to calcareous soils, its sensitivity to the citrus tristeza virus (CTV), one of the most serious biotic stressors

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Lili Dong, Tongrui Liu, Di Gao, Jing Li, and Jie Qian

), with the temperature of 23 ± 2 °C, the illumination of 100 µmol·m −2 ·s ‒1 , and the photoperiod of 16/8 h (light/dark). Isolation of the PhSDG8 gene. Total RNA was extracted from stems of petunia plants using EZ-10 Total RNA Mini-Preps Kit (Sangon

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Yihua Chen, Peng Jiang, Shivegowda Thammannagowda, Haiying Liang, and H. Dayton Wilde

collected in November and March. Seedling material was obtained from two-month-old plants maintained in a growth room (22 °C, 16 h light/8 h dark). Total RNA was isolated from peach and arabidopsis tissues using the RNeasy Plant Mini Kit (Qiagen, Germantown

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Jiyu Zhang, Min Wang, Zhenghai Mo, Gang Wang, and Zhongren Guo

immediately frozen in liquid nitrogen and stored at −80 °C until used. Cloning of CiAG . Total RNA was extracted from the staminate flowers of ‘Mahan’ using The Plant Total RNA Extraction Kit (BioTeke, Beijing, China). First-strand cDNA was synthesized from 1

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Hua Zhou, Fang-Yun Cheng, Jing Wu, and Chaoying He

reaction (RT-PCR) with total RNA extracted from the opened flowers. Total RNA was extracted with the RN38-EASYspin Plus (Aidlab, Beijing, China) and treated with RNase-free DNase I (Promega, Madison, WI). Total RNA purity was assessed using a