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Loren C. Stephens

culture has been a successful alternative approach for plants not prone to somaclonal variation ( Ahloowalia, 1998 ). One such mutagen, ethidium bromide (EtBr), has been used successfully as a seed soak ( Burton and Hanna, 1976 ), but there are no reports

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Paul M. Lyrene

origin and taxonomy of citrus and germplasm resources. There are chapters on haploidy, cytogenetics, and ploidy manipulation. About half of the chapters I would classify as biotechnology–molecular biology, including chapters on somaclonal variation

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Juanxu Liu, Min Deng, Richard J. Henny, Jianjun Chen, and Jiahua Xie

, micropropagation of dracaenas has been hampered by low multiplication rates and high occurrence of somaclonal variation. The numbers of shoot tips or axillary buds are often limited in dracaenas, and their use as explants produced limited numbers of shoots ( Miller

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Xiaoming Wang, Yongxin Li, Huijie Zeng, Neng Cai, Zhongquan Qiao, Xiangying Wang, and Jianjun Chen

through a regeneration process; somaclonal variation is generally not expected ( Chen and Henny, 2006 ). The established protocol has been used for propagation of ‘Tango’ for more than 2 years in our laboratory. It produces repeatable results in weigela

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Wenhao Dai and Victoria Magnusson

speed establishment of stable periclinal chimeras; plant regeneration from single undifferentiated cell lines may circumvent problems associated with the formation of chimeras; and an in vitro system itself is a source of mutations (somaclonal variations

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Alejandro Martínez Palacios, Raúl Cárdenas Navarro, Diana Beatriz Hernández Ortega, and Víctor Chávez Avila

explants and that this PGR involves the risk of somaclonal variation associated with callus development. Such results point out the necessity to explore the potential of other PGRs to induce the axillary bud development in stem explants of T. valdezianus

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Saad B. Javed, Abdulrahman A. Alatar, Mohammad Anis, and Mohamed A. El-Sheikh

plants, known as somaclonal variations ( Larkin and Scowcroft, 1981 ). The chances of somaclonal variations are increased if the cultures are exposed to stress. For micropropagation, clonal fidelity of the regenerants is an important criterion ( Roy et al

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Jennifer A. Kimball, Thomas G. Isleib, William C. Reynolds, Maria C. Zuleta, and Susana R. Milla-Lewis

Milla-Lewis, S.R. 2016b Freeze-testing in St. Augustinegrass II: Evaluation of acclimation effects Eur. J. Agron. (In review) Lee, M. Phillips, R.L. 1988 The chromosomal basis of somaclonal variation Annu. Rev. Plant Physiol. Plant Mol. Biol. 39 413 437

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José R. Bautista-Aguilar, Lourdes G. Iglesias-Andreu, Jaime Martínez-Castillo, Marco A. Ramírez-Mosqueda, and Matilde M. Ortiz-García

process, somaclonal variants may arise that cause alterations in the genetic homogeneity of the conserved germplasm ( Tyagi et al., 2010 ). In V. planifolia, there are few studies on the analysis of somaclonal variation in micropropagated plants. In this

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Yeun-Kyung Chang, Richard E. Veilleux, and Muhammad Javed Iqbal

( Tokuhara and Mii, 1993 ). Somaclonal variants with altered phenotypes have been reported to occur in a range of 0% to 14% ( Tokuhara and Mii, 1998 , 2001 ). Somaclonal variation at the molecular level has been documented in Phalaenopsis using RAPD ( Chen